プロフィール詳細

プロジェクトを作成

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Dr. Amol S.に依頼

United States

Protein engineer with 15+ years of academic and industry experience

プロフィール概要

• Experienced leader with extensive expertise in protein engineering, antibody engineering, biosensors, and peptide discovery. Proven ability to build and lead high-performing teams by integrating directed evolution, computational modeling, automation & machine learning into biologics discovery. Passionate about developing novel biologics and collaborating across disciplines to drive innovation. • Biomolecules Discovered/Engineered: phytase, xylanase, masked-antibody, biosensors for neuromodulators and CNS drugs, cyclic peptide. Products/Processes Launched Xylanase as Frontia® Prime by Novozymes, Denmark (valued at USD $19.5B) Phytase as Natuphos® E by BASF, Germany (valued at USD $510M) Bioprocess for commercial manufacturing two APIs at Pfizer, USA (>$15M savings/year)

専門分野

• ☆ Biocatalysis
• ☆ Molecular Modeling
• ☆ Molecular Docking
• ☆ Molecular Biology
• ☆ Molecular Evolution
• ☆ PCR
• ☆ Molecular Cloning
• ☆ Biotechnology & Bioengineering
• ☆ Recombinant Protein Expression
• ☆ Data Analysis
• ☆ Computer Simulation/Computational Modeling
• ☆ Biomolecular Engineering
• ☆ Protein/Enzyme Engineering

サービス

• Research
• Consulting

Research Feasibility Study, Technology Scouting, Fact Checking, Scientific and Technical Research, Systematic Literature Review

Consulting Scientific and Technical Consulting

職務経験

Manager/Team Lead

Pfizer

11月 2022 - 現在

Senior Scientist

Howard Hughes Medical Institute

6月 2021 - 現在

Senior Scientist

California Institute of Technology

12月 2019 - 6月 2021

Senior Scientist

Novozymes South Asia Pvt Ltd

6月 2018 - 10月 2019

Senior postdoctoral fellow

California Institute of Technology

2月 2014 - 5月 2018

Postdoctoral fellow

University of California Santa Barbara

2月 2012 - 2月 2014

学歴

PhD (Biochemical Engineering)

Jacobs University Bremen gGmbH

11月 2007 - 12月 2010

M.S. (Pharm) (Biotechnology)

National Institute of Pharmaceutical Education and Research

6月 2005 - 6月 2007

認定資格

• 認定資格の詳細は未入力です。

出版物

JOURNAL ARTICLE

Unger EK, Keller JP, Altermatt M, Liang R, Matsui A, Dong C, Hon OJ, Yao Z, Sun J, Banala S, et al.(2020). Directed Evolution of a Selective and Sensitive Serotonin Sensor via Machine Learning . Cell.

Bera K, Kamajaya A, Shivange AV, Muthusamy AK, Nichols AL, Borden PM, Grant S, Jeon J, Lin E, Bishara I, et al.(2019). Biosensors Show the Pharmacokinetics of S-Ketamine in the Endoplasmic Reticulum . Frontiers in cellular neuroscience.

Kurdekar V, Giridharan S, Subbarao J, Nijaguna MB, Periasamy J, Boggaram S, Shivange AV, Sadasivam G, Padigaru M, Potluri V, et al.(2019). Structure-Guided Synthesis and Evaluation of Small-Molecule Inhibitors Targeting Protein-Protein Interactions of BRCA1 tBRCT Domain . ChemMedChem.

Shivange AV, Borden PM, Muthusamy AK, Nichols AL, Bera K, Bao H, Bishara I, Jeon J, Mulcahy MJ, Cohen B, et al.(2019). Determining the pharmacokinetics of nicotinic drugs in the endoplasmic reticulum using biosensors . The Journal of general physiology.

Shivange AV, Hoeffken HW, Haefner S, Schwaneberg U(2016). Protein consensus-based surface engineering (ProCoS): a computer-assisted method for directed protein evolution . BioTechniques.

Shivange, A.V., Roccatano, D., Schwaneberg, U.(2016). Iterative key-residues interrogation of a phytase with thermostability increasing substitutions identified in directed evolution . Applied Microbiology and Biotechnology. 100. (1). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 227-242.

Shivange, A.V., Hoeffken, H.W., Haefner, S., Schwaneberg, U.(2016). Protein consensus-based surface engineering (Procos): A computer-assisted method for directed protein evolution . BioTechniques. 61. (6). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 305-314.

Shivange AV, Roccatano D, Schwaneberg U(2015). Iterative key-residues interrogation of a phytase with thermostability increasing substitutions identified in directed evolution . Applied microbiology and biotechnology.

Shivange AV, Daugherty PS(2015). De novo discovery of bioactive cyclic peptides using bacterial display and flow cytometry . Methods in molecular biology (Clifton, N.J.).

Belsare KD, Ruff AJ, Martinez R, Shivange AV, Mundhada H, Holtmann D, Schrader J, Schwaneberg U(2014). P-LinK: A method for generating multicomponent cytochrome P450 fusions with variable linker length . BioTechniques.

Belsare, K.D., Joëlle Ruff, A., Martinez, R., Shivange, A.V., Mundhada, H., Holtmann, D., Schrader, J., Schwaneberg, U.(2014). P-LinK: A method for generating multicomponent cytochrome P450 fusions with variable linker length . BioTechniques. 57. (1). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 13-20.

Shivange, A.V., Dennig, A., Schwaneberg, U.(2014). Multi-site saturation by OmniChange yields a pH- and thermally improved phytase . Journal of Biotechnology. 170. (1). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 68-72.

Dudek, H.M., Fink, M.J., Shivange, A.V., Dennig, A., Mihovilovic, M.D., Schwaneberg, U., Fraaije, M.W.(2014). Extending the substrate scope of a Baeyer-Villiger monooxygenase by multiple-site mutagenesis . Applied Microbiology and Biotechnology. 98. (9). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 4009-4020.

Shivange AV, Dennig A, Schwaneberg U(2013). Multi-site saturation by OmniChange yields a pH- and thermally improved phytase . Journal of biotechnology.

Dudek HM, Fink MJ, Shivange AV, Dennig A, Mihovilovic MD, Schwaneberg U, Fraaije MW(2013). Extending the substrate scope of a Baeyer-Villiger monooxygenase by multiple-site mutagenesis . Applied microbiology and biotechnology.

Jakoblinnert A, van den Wittenboer A, Shivange AV, Bocola M, Heffele L, Ansorge-Schumacher M, Schwaneberg U(2013). Design of an activity and stability improved carbonyl reductase from Candida parapsilosis . Journal of biotechnology.

Jakoblinnert A, Wachtmeister J, Schukur L, Shivange AV, Bocola M, Ansorge-Schumacher MB, Schwaneberg U(2013). Reengineered carbonyl reductase for reducing methyl-substituted cyclohexanones . Protein engineering, design & selection : PEDS.

Jakoblinnert, A., Wachtmeister, J., Schukur, L., Shivange, A.V., Bocola, M., Ansorge-Schumacher, M.B., Schwaneberg, U.(2013). Reengineered carbonyl reductase for reducing methyl-substituted cyclohexanones . Protein Engineering, Design and Selection. 26. (4). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 291-298.

Jakoblinnert, A., van den Wittenboer, A., Shivange, A.V., Bocola, M., Heffele, L., Ansorge-Schumacher, M., Schwaneberg, U.(2013). Design of an activity and stability improved carbonyl reductase from Candida parapsilosis . Journal of Biotechnology. 165. (1). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 52-62.

Ruff, A.J., Marienhagen, J., Verma, R., Roccatano, D., Genieser, H.-G., Niemann, P., Shivange, A.V., Schwaneberg, U.(2012). DRTP and dPTP a complementary nucleotide couple for the Sequence Saturation Mutagenesis (SeSaM) method . Journal of Molecular Catalysis B: Enzymatic. 84. Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 40-47.

Shivange, A.V., Serwe, A., Dennig, A., Roccatano, D., Haefner, S., Schwaneberg, U.(2012). Directed evolution of a highly active Yersinia mollaretii phytase . Applied Microbiology and Biotechnology. 95. (2). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 405-418.

Shivange AV, Serwe A, Dennig A, Roccatano D, Haefner S, Schwaneberg U(2011). Directed evolution of a highly active Yersinia mollaretii phytase . Applied microbiology and biotechnology.

Dennig A, Shivange AV, Marienhagen J, Schwaneberg U(2011). OmniChange: the sequence independent method for simultaneous site-saturation of five codons . PloS one.

Dennig, A., Shivange, A.V., Marienhagen, J., Schwaneberg, U.(2011). Omnichange: The sequence independent method for simultaneous site-saturation of five codons . PLoS ONE. 6. (10).

Shivange AV, Schwaneberg U, Roccatano D(2010). Conformational dynamics of active site loop in Escherichia coli phytase . Biopolymers.

Shivange, A.V., Schwaneberg, U., Roccatano, D.(2010). Conformational dynamics of active site loop in Escherichia coli phytase . Biopolymers. 93. (11). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 994-1002.

Kaur, S., Shivange, A.V., Roy, N.(2010). Structural analysis of trypanosomal sirtuin: An insight for selective drug design . Molecular Diversity. 14. (1). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 169-178.

Kaur S, Shivange AV, Roy N(2009). Structural analysis of trypanosomal sirtuin: an insight for selective drug design . Molecular diversity.

Shivange AV, Marienhagen J, Mundhada H, Schenk A, Schwaneberg U(2009). Advances in generating functional diversity for directed protein evolution . Current opinion in chemical biology.

Shivange, A.V., Marienhagen, J., Mundhada, H., Schenk, A., Schwaneberg, U.(2009). Advances in generating functional diversity for directed protein evolution . Current Opinion in Chemical Biology. 13. (1). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 19-25.

Prusty D, Mehra P, Srivastava S, Shivange AV, Gupta A, Roy N, Dhar SK(2008). Nicotinamide inhibits Plasmodium falciparum Sir2 activity in vitro and parasite growth . FEMS microbiology letters.

Prusty, D., Mehra, P., Srivastava, S., Shivange, A.V., Gupta, A., Roy, N., Dhar, S.K.(2008). Nicotinamide inhibits Plasmodium falciparum Sir2 activity in vitro and parasite growth . FEMS Microbiology Letters. 282. (2). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 266-272.

Kadam RU, Shivange AV, Roy N(2007). Escherichia coli versus Pseudomonas aeruginosa deacetylase LpxC inhibitors selectivity: surface and cavity-depth-based analysis . Journal of chemical information and modeling.

Kadam, R.U., Shivange, A.V., Roy, N.(2007). Escherichia coli versus Pseudomonas aeruginosa deacetylase LpxC inhibitors selectivity: Surface and cavity-depth-based analysis . Journal of Chemical Information and Modeling. 47. (3). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 1215-1224.

PREPRINT

Philip M. Borden, Peng Zhang, Amol V. Shivange, Jonathan S. Marvin, Joseph Cichon, Chuntao Dan, Kaspar Podgorski, Antonio Figueiredo, Ondrej Novak, Masashi Tanimoto, et al. (2020). A fast genetically encoded fluorescent sensor for faithfulin vivoacetylcholine detection in mice, fish, worms and flies .

Kurdekar V, Giridharan S, Subbarao J, Nijaguna MB, Periasamy J, Boggaram S, Bharatham K, Potluri V, Shivange AV, Sadasivam G, et al.(2019). Structural features underlying the activity of benzimidazole derivatives that target phosphopeptide recognition by the tandem BRCT domain of the BRCA1 protein .

OTHER

A.V. Shivange, P.M. Borden(2019). Crystal Structure of iNicSnFR 1.0 . Worldwide Protein Data Bank

Amol V. Shivange, Ulrich Schwaneberg(2017). Recent Advances in Directed Phytase Evolution and Rational Phytase Engineering . Directed Enzyme Evolution: Advances and Applications. Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 145--172. Springer International Publishing

BOOK CHAPTER

Shivange, A.V., Schwaneberg, U.(2017). Recent advances in directed phytase evolution and rational phytase engineering . Directed Enzyme Evolution: Advances and Applications. Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 145-172.

BOOK

Shivange, A.V., Daugherty, P.S.(2015). De novo discovery of bioactive cyclic peptides using bacterial display and flow cytometry . Methods in Molecular Biology. 1248. Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 139-153.