プロフィール詳細

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Dr. ATUL S.に依頼

India

Freelance PhD Holder, having 10+ years wet lab experience. Specialization in immunology and Gene cloning

プロフィール概要

I am a PhD Degree holder Botany. My degree was awarded in year 2022. My PhD topic was Immunomodulatory role of O.sanctum Linn (Black tulsi) on smoke induced chronic obstructive pulmonary disorder. Awarded ICMR SRF year 2017 (File No.: xxxxxxxxxx/BMS/TRM) for the project entitled “Immunomodulatory Role of Ocimum sanctum Linn Black Tulsi on smoke induced Chronic obstructive pulmonary disorder (COPD)” Duration September 2017 to August 2021 RESEARCH INTEREST: o Ethnobotany o Medicinal plants and their Phytochemical analysis o Immunology of chronic airway Inflammation. o Immunomodulation. o Role of Oxidants and Antioxidants in Stress Biology o Signalling pathways in chronic inflammatory disease RESEARCH EXPERIENCE: Institute of Medical Sciences, Banaras Hindu University India UP Varanasi 221005 PhD Scholar  Design and optimize the smoke chamber for active smoking in Mice.  Experimental design to generate the COPD in Mice.  Extract preparation with the help of Soxhlet apparatus  GCMS analysis and comparison of data with Wiley online library to study the presence of various phytochemicals in the leaf extract.  Histopathology: To study the various pathological architectural changes in the lungs microtomy was performed o Tissue Fixation, Block formation and tissue cutting with the help of Microtome o Different type ofstaining  Hematoxylin and Eosin for general pathological changes  Masson trichome staining for extracellular matrix detection as collagen  Periodic Acid Schiff staining for mucus and goblet cells  Sirus red staining for collagen deposition  Cellular Techniques o Differential cell count, Total cell count, and cell percentage by cytospin o Retrieving Bronchoalveolar lavage fluid (BALF) from Lung washing o Cytospun of BALF for inflammatory cell count by Giemsa stain o Determination of cell viability by Trypan blue dye exclusion test  Immunological Techniques o ELISA for detecting cytokines (IL6, IL8, IL10, IL17A, IFNγ, TNFα in BALF. o Immune cells phenotyping/profiling  Molecular techniques o Zymography o Gel electrophoresis o Western blotting for enzymatic expression of Map kinase (Erk, JNK,p38) o Flow cytometry for cell Phenotyping and Apoptotic study o PCR and Real Time PCR (qPCR)  Biochemical techniques o Protein estimation by Lowery method o Assay of various antioxidants enzymes as SOD, Catalase, LPO, MPO, EPO, NE by spectrophotometer o Level of TAC, TOS, ROS, MDA, by ELISA plate reader o Estimation of Nitrate for Nitric oxide by Griess Reagent. o In-vivo cytotoxic effect of extract on liver and kidney by estimating AST ALT and creatinine level  Bioinformatics Tools o ADMET analysis by SwissADME and ADMETlab 2.0 o Molecular docking (Protein ligand and Protein-Protein) by Autodoc o Network pharmacology Analysis by analysing target gene and disease gene through databases and Construction of compound-target network by cytoscape o Gene Ontology (GO) gene enrichment analysis and Kyoto encyclopaedia of genes and genomes (KEGG) pathway annotation by Cytoscape and STRING  Animal Handling o Maintaining colony of inbred strain (Balb/c mice) o Intra peritoneal, Intra nasal, Intra venous administration of drug o Blood extraction by retro orbital plexus  Scientific writing  Good laboratory practice

専門分野

• ★ Oxidative Stress & Mitochondrial Function
• ★ Antioxidants
• ★ Molecular Biology
• ☆ Botany
• ☆ Microbiology
• ☆ Mycology/Fungi
• ☆ Biostatistics
• ☆ Biochemistry
• ☆ Immunoassay
• ☆ Enzyme Activity Assays
• ☆ PCR
• ☆ Cell Biology
• ☆ Cell Signaling Pathways
• ☆ Histology
• ☆ Pathogenesis
• ☆ Clinical Microbiology
• ☆ Immunohistochemistry
• ☆ R
• ☆ Drug Development
• ☆ Microbiology Testing
• ☆ Inflammation

サービス

• Writing
• Research
• Consulting
• Data & AI
• Product Development

Writing Copywriting, Creative Writing, General Proofreading & Editing, Translation

Research Scientific and Technical Research, Systematic Literature Review

Consulting Scientific and Technical Consulting

Data & AI Statistical Analysis

Product Development Formulation, Concept Development

職務経験

RJ-PDF

Banaras Hindu University

1月 2024 - 現在

学歴

Phd (Biochemistry IMS )

Banaras Hindu University

9月 2017 - 現在

PhD Botany

Banaras Hindu University

9月 2017 - 3月 2022

認定資格

• 認定資格の詳細は未入力です。

出版物

JOURNAL ARTICLE

Atul Srivastava, Vandana Yadav, Vinita Pandey, Pratikkumar Gaglani, Soni, Subhashini (2025). Network pharmacology and molecular docking insights into Sirtuin-2 inhibitors (AK-7 and AGK-2): A synergistic strategy for COPD treatment . Human Gene.

Atul Srivastava, Vandana Yadav, Vinita Pandey, Pratikkumar Gaglani, Soni, Subhashini (2024). Inhibiting SIRT-2 by AK-7 restrains airway inflammation and oxidative damage promoting lung resurgence through NF-kB and MAP kinase signaling pathway . Frontiers in Immunology.

Atul Srivastava, Vinita Pandey, Vandana Yadav, Pratikkumar Gaglani, Rashmi Singh, Subhashini (2024). Blocking μ-opioid receptor by naltrexone exaggerates oxidative stress and airway inflammation via the MAPkinase pathway in a murine model of asthma . Free Radical Biology and Medicine.

Atul Srivastava, Vandana Yadav, Vinita Pandey, Sangita Singh, Subhashini (2023). Pharmacological inhibition of SIRT-2 by AK-7 modulates redox status and apoptosis via regulating Nrf2 in an experimental model of chronic obstructive pulmonary disease: an invivo and insilico study . Free Radical Research.

Atul Srivastava, Vinita Pandey, Vandana Yadav, Rashmi Singh, Subhashini (2023). β-Endorphin (an endogenous opioid) inhibits inflammation, oxidative stress and apoptosis via Nrf-2 in asthmatic murine model . Scientific Reports.

Atul Srivastava, Subhashini, Vinita Pandey, Vandana Yadav, Sangita Singh, Ragini Srivastava (2023). Potential of hydroethanolic leaf extract of Ocimum sanctum in ameliorating redox status and lung injury in COPD: an in vivo and in silico study . Scientific Reports.

Atul Srivastava, Subhashini Subhashini S, Anand Kumar Keshari, Ragini Srivastava (2021). Phytochemical and GC-MS Analysis of Hydro Ethanolic Leaf Extract of Ocimum sanctum (L.) . Pharmacognosy Research.

Atul Srivastava (2021). Antioxidant and Antibacterial Property of Biosynthesised Silver Nanoparticles .

Atul Srivastava, Anand Keshari, Sandip Chowdhury, Ragini Srivastava (2021). Green Synthesis of Silver Nanoparticles using Catharanthus roseus: Its Antioxidant and Antibacterial Properties . Nanomedicine Research Journal.

Atul Srivastava, Anand Keshari, Akhilesh Verma, Ragini Srivastava (2016). Free Radicals Scavenging and Protein Protective Property of Ocimum sanctum (L) . British Journal of Pharmaceutical Research.

Atul Srivastava, Anand Keshari, Akhilesh Verma, Ragini Srivastava (2016). Free Radicals Scavenging and Protein Protective Property of Ocimum sanctum (L) . British Journal of Pharmaceutical Research.

Atul Srivastava Ocimum inhibits airway inflammation in cigarette smoke induced COPD.

BOOK CHAPTER

Atul Srivastava, Mrinalini Kumari, Shyam Babu Sah, Subhashini (2022). Biological Control of Agricultural Insect Pests . Insecticides - Impact and Benefits of Its Use for Humanity.

Atul Srivastava, Mrinalini Kumari, Dinesh Prasad Gond, Subhashini (2020). Basic overview of human physiology . Smart Healthcare for Disease Diagnosis and Prevention.