プロフィール詳細

プロジェクトを作成

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Dr. Dinesh L.に依頼

India

Research Scientist | 16+ yrs in Plant Tissue Culture, Molecular Biology, Microbial Enzymes & Protein Purification

プロフィール概要

With over 16 years of experience in plant biotechnology, microbiology, enzyme production, and R&D leadership, I bring a strong foundation in scientific innovation, regulatory compliance, and process optimization. My expertise spans: Bioprocess development for enzyme and peptide production, including strain improvement, media optimization, and scale-up. Protein purification techniques: affinity, ion-exchange, and size-exclusion chromatography. Molecular biology and microbial screening for bioactive compound discovery. Plant tissue culture and regeneration systems. Design of Experiments (DoE) for process optimization and data-driven decision-making. Quality Management Systems (ISO xxxxxxxxx) and regulatory documentation for DBT and DSIR. Scientific writing and documentation, including technical reports, regulatory submissions (DBT/DSIR), SOPs, patents, and research publications—ensuring clarity, compliance, and knowledge dissemination. Proficient in analytical and documentation tools essential for regulated environments.

専門分野

• ☆ Microbiology
• ☆ Agricultural Microbiology
• ☆ Biotechnology & Bioengineering
• ☆ Industrial Biotechnology
• ☆ Agricultural Biotechnology
• ☆ Leadership

サービス

• Writing
• Consulting
• Product Development

Writing Non-Medical Regulatory Writing, Technical Writing, Copywriting, Creative Writing

Consulting Scientific and Technical Consulting

Product Development Recipe Development, Product Evaluation, Product Validation

職務経験

Head

Dr. D.Y. Patil Vidyapeeth, Pune

5月 2012 - 現在

R&D Head

Rise N Shine Biotech PVT. LTD

5月 2012 - 1月 2025

学歴

Doctor of Philosophy (Ph.D)

Dr. D.Y. Patil University - India

4月 2017 - 1月 2025

M.Sc.

Kerala Agriculture University, Thrissur

8月 2006 - 4月 2009

B.Sc.

Marathwada Agricultural University - India

8月 2000 - 6月 2004

認定資格

• Three-Day Workshop on Research Data Analysis & Interpretation using SPSS

  A2Z EduLearningHub

  3月 2022 - 現在

出版物

JOURNAL ARTICLE

Efficient regeneration of Stevia rebaudiana Bertoni transformants through hairy root culture technique @article{Efficient regeneration of Stevia rebaudiana Bertoni transformants through hairy root culture technique_2025, volume={98}, url={https://ojs.openagrar.de/index.php/JABFQ/article/view/17814}, DOI={10.5073/JABFQ.2025.098.003}, abstractNote={ Stevia rebaudiana is a small herb from the Asteraceae family. The plant produces tetracyclic diterpenes, steviol glycosides (SGs) that makes it Stevia sweet. Stevia can act as a natural sweetener as it does not interfere with insulin metabolism in humans when consumed. Amid the prevailing paradigm of health-focused living, Stevia can be produced and used worldwide by individuals and plant breeders. Yet, it is frequently challenging to cultivate stevia conventionally due to low germinating rate and viability of its seeds. Hence, raising plants through tissue culture becomes a viable option. The present study aims to regenerate transformed Stevia lines through Rhizobium rhizogenes mediated hairy root culture for investigating the possibility of increasing SGs. This study also focuses on increasing Stevia plant lines through an efficient and cost-effective micropropagation technique. For micropropagation, MS media supplied with 1 mg L-1 BAP in combination with 0.2 mg L -1 NAA gave the best result for shooting with nodal segments as explants. MS medium supplemented with IBA at a concentration of 1 mg L-1 was most effective in promoting the highest number of roots, while 2 mg L-1 IBA was optimal for achieving the longest root length. The highest number and frequency of hairy roots were observed with co-culture period of 48 h. Growth of transformed plantlets was observed best with 1 mg L-1 BAP + 0.3 mg L-1 NAA after 8 weeks of micro-shoots placed on solid MS media. Stevia plants raised through these biotechnological approaches will be a boon to plant breeders and researchers as these are efficient and easy methods to raise Stevia plants. The growing demand for natural sweeteners will be met at lower cost to pharmacology industries and individuals suffering from various life style diseases such as diabetes, hypertension and obesity. }, journal={Journal of Applied Botany and Food Quality}, year={2025}, month={Mar.}, pages={22–28} } . Journal of Applied Botany and Food Quality.