プロフィール詳細

プロジェクトを作成

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Dr. Eamon B.に依頼

United States

Life Sciences Consultant | Oxford, Stanford-trained | Protein engineering, ML, CAR-T, CRISPR, lentivirus, GPCRs, HTS

プロフィール概要

My background is in the structural biology of membrane proteins, specifically G-protein coupled receptors (GPCRs). During my DPhil (PhD), I solved the first structure of a GPCR with a large, functional extracellular domain using X-ray crystallography. This protein is called "Smoothened" and it is a drug target relevant to various types of cancer. Smoothened is a part of the Hedgehog signalling pathway, which is essential for proper embryonic development in all animals. I have substantial experience in protein production, purification, biophysical characterization and crystallization, with a particular focus on difficult membrane-bound targets and drugs that they interact with. I also have experience in methods development and assay development using purified protein components. My writing and editing skills are excellent. I have experience writing and editing scientific manuscripts for publication in top scientific journals, including Nature, Cell and eLife.

専門分野

• ☆ Separation & Purification
• ☆ Crystallography
• ☆ X-Ray Crystallography
• ☆ Developmental Biology
• ☆ Biochemical Assays
• ☆ Structural Biology
• ☆ Cell Signaling Pathways
• ☆ Biophysics
• ☆ Scientific Proofreading & Editing

サービス

• Consulting
• Product Development

Consulting Scientific and Technical Consulting

職務経験

Postdoctoral Research Fellow

Stanford University

8月 2018 - 現在

Postdoctoral Research Scientist

University of Oxford

10月 2017 - 7月 2018

学歴

(NDM Experimental Medicine; University College)

University of Oxford

- 現在

DPhil (Clinical Medicine)

University of Oxford

10月 2013 - 現在

Undergraduate Honours (Structural Biology)

Walter and Eliza Hall Institute of Medical Research

2月 2012 - 10月 2012

Bachelor of Arts/Bachelor of Science

University of Melbourne

2月 2007 - 11月 2011

認定資格

• 認定資格の詳細は未入力です。

出版物

JOURNAL ARTICLE

Raleigh DR, Sever N, Choksi PK, Sigg MA, Hines KM, Thompson BM, Elnatan D, Jaishankar P, Bisignano P, Garcia-Gonzalo FR, et al.(2018). Cilia-Associated Oxysterols Activate Smoothened . Molecular cell.

Eamon FX Byrne, Giovanni Luchetti, Rajat Rohatgi, Christian Siebold(2018). Multiple ligand binding sites regulate the Hedgehog signal transducer Smoothened in vertebrates . Current Opinion in Cell Biology. 51. Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 81--88. Elsevier {BV}

Cyrus Tan, Eamon F. X. Byrne, Casey Ah-Cann, Melissa J. Call, Matthew E. Call(2018). A serine in the first transmembrane domain of the human E3 ubiquitin ligase {MARCH}9 is critical for down-regulation of its protein substrates . Journal of Biological Chemistry. 294. (7). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 2470--2485. American Society for Biochemistry {\&} Molecular Biology ({ASBMB})

Luchetti G, Sircar R, Kong JH, Nachtergaele S, Sagner A, Byrne EF, Covey DF, Siebold C, Rohatgi R, eLife, 2016, vol. 5, 2016 .

Eamon F. X. Byrne, Ria Sircar, Paul S. Miller, George Hedger, Giovanni Luchetti, Sigrid Nachtergaele, Mark D. Tully, Laurel Mydock-McGrane, Douglas F. Covey, Robert P. Rambo, et al.(2016). Structural basis of Smoothened regulation by its extracellular domains . Nature. Springer Nature

Sharma, P., Kaywan-Lutfi, M., Krshnan, L., Byrne, E. F. X., Call, M. J., Call, M. E. Production of Disulfide-stabilized Transmembrane Peptide Complexes for Structural Studies. J. Vis. Exp. (73), e50141, doi:10.3791/50141 (2013) .