プロフィール詳細

プロジェクトを作成

★★★★★

☆☆☆☆☆

★★★★★

☆☆☆☆☆

Dr. Flavia A.に依頼

United States

Biomedical Scientist | Preclinical Research | Skeletal Biology | Experimental Design | Scientific Consultant

プロフィール概要

Ph.D. Biomedical Scientist with extensive experience in skeletal biology, biomineralization, ectopic calcification, molecular and cellular biology, and translational research. I specialize in experimental design, animal models, histology, immunohistochemistry, molecular biology techniques, microscopy, data analysis, and scientific writing. I provide consulting services for study design, data interpretation, manuscript and grant review, scientific editing, and preclinical research support for academic and industry clients.

専門分野

• ☆ Animal Science
• ☆ Biochemistry
• ☆ Enzyme Activity Assays
• ☆ Molecular Biology
• ☆ Omics
• ☆ Proteomics
• ☆ PCR
• ☆ Gene Expression Analysis
• ☆ Cell Biology
• ☆ Cell-Based Assays
• ☆ Cell Signaling Pathways
• ☆ Stem Cell Research
• ☆ Histology
• ☆ Cell Culture
• ☆ Primary Cells
• ☆ Cell Banking
• ☆ Drug Discovery
• ☆ Drug Development
• ☆ Preclinical Research
• ☆ Gene Therapy
• ☆ Medical Sciences

サービス

• Writing
• Research
• Consulting
• Data & AI

Writing Technical Writing, General Proofreading & Editing

Research Feasibility Study, Fact Checking, Scientific and Technical Research

Consulting Healthcare Consulting, Scientific and Technical Consulting

Data & AI Statistical Analysis, Image Analysis, Data Visualization, Data Insights

職務経験

Staff Scientist

Sanford Burnham Prebys Medical Discovery Institute

8月 2023 - 現在

Postdoctoral Associate

Sanford Burnham Prebys Medical Discovery Institute

11月 2021 - 7月 2023

Research Fellow

Harvard School of Dental Medicine

5月 2021 - 10月 2021

Postdoctoral Associate

Sanford Burnham Prebys Medical Discovery Institute

4月 2018 - 4月 2021

学歴

Ph.D. in Science (Department of Biological Sciences)

Universidade de São Paulo

12月 2013 - 1月 2018

Research Scholar (Department of Pharmacology)

UNIVERSITY OF CAMBRIDGE

2月 2016 - 8月 2016

Research Scholar (Department of Biotechnological and Applied Clinical Sciences)

University of L'Aquila

4月 2014 - 4月 2015

M.Sc. in Science (Department of Biological Sciences)

Universidade de São Paulo

8月 2011 - 11月 2013

認定資格

• Working with Mice in Research Settings

  Sanford Burnham Prebys Medical Discovery Institute

  1月 2025 - 現在

• Responsible Conduct of Research

  Sanford Burnham Prebys Medical Discovery Institute

  1月 2026 - 12月 2026

出版物

JOURNAL ARTICLE

Mariana Liessa Rovis Sanches, Cintia Kazuko Tokuhara, Flávia Amadeu de Oliveira, Talita Ventura, Adriano de Souza Pessoa, Flávia Godoy Iano, Mariana Rodrigues Santesso, Emanuelle Pangoni de Carvalho, Larissa Tercília Grizzo Thomassian, Marília Rabelo Buzalaf, et al. (2026). Crosstalk between adipocytes and osteoblasts in regulating bone differentiation . Molecular and Cellular Endocrinology.

Cintia Tokuhara, Flavia Amadeu de Oliveira, Sonoko Narisawa, Elis J Lira dos Santos, Brian L Foster, Jose Luis Millan (2026). Effects of comorbid chronic kidney disease on late-onset hypophosphatasia mice under treatment with asfotase alfa . JBMR Plus.

Sonoko Narisawa, Flavia Amadeu de Oliveira, Cintia Kazuko Tokuhara, Elis J Lira dos Santos, Elena Fonfria, Jennifer Batson, Zhiliang Cheng, Ann Houston, Brian L Foster, José Luis Millán (2026). ENPP1 inhibition as a therapeutic approach for later-onset hypophosphatasia . Journal of Bone and Mineral Research.

Aonjittra Phanrungsuwan, Bella Donnelly, Jinju Kim, Jack Sweet, Flavia Amadeu de Oliveira, Do-Gyoon Kim, Eva S Liu, José Luis Millán, Marc D McKee, Brian L Foster (2025). Genetic reduction or deletion of osteopontin in osteomalacic Hyp mice fails to ameliorate dentoalveolar defects . JBMR Plus.

Aonjittra Phanrungsuwan, Bella Donnelly, Fatma F Mohamed, Kedith Sawangsri, Karli Belgya, Tamara N Kolli, Jinju Kim, Do-Gyoon Kim, Flavia Amadeu de Oliveira, Eva S Liu, et al. (2025). Proof-of-principle for enhanced dentoalveolar mineralization using exogenous tissue-nonspecific alkaline phosphatase in the Hyp mouse model of X-linked hypophosphatemia . JBMR Plus.

F.F. Mohamed, A. Phanrungsuwan, F. Amadeu de Oliveira, J.L. Millán, B.L. Foster (2025). Dentoalveolar defects and impaired alveolar bone healing in a neural crest directed conditional knockout mouse model of hypophosphatasia . Bone.

Flavia Amadeu de Oliveira, Cintia Kazuko Tokuhara, Fatma F Mohamed, Sonoko Narisawa, Elis J Lira dos Santos, Natalie L Andras, Mohammad Shadid, Koichi Miyake, Brian Foster, Jose Luis Millan (2025). Preclinical evaluation of the efficacy and safety of adeno-associated virus 8-tissue-nonspecific alkaline phosphatase-D10 in Alpl-/- and AlplPrx1/Prx1 mouse models for the treatment of early and late-onset hypophosphatasia . Journal of Bone and Mineral Research.

Kelly Karina Inacio, Adriano de Souza Pessoa, Cintia Kazuko Tokuhara, Ana Lígia Pagnan, Mariana Liessa Rovis Sanches, Vanessa Svizzero Fakhoury, Gabriela Silva Neubern de Oliveira, Flavia Amadeu de Oliveira, Valdecir Farias Ximenes, Rodrigo Cardoso de Oliveira (2024). Menadione and protocatechuic acid: A drug combination with antitumor effects in murine osteosarcoma cells . Archives of Biochemistry and Biophysics.

Fatma F. Mohamed, Flavia Amadeu de Oliveira, Yuka Kinoshita, Riti R. Yalamanchili, Leena A. Eltilib, Natalie L. Andras, Sonoko Narisawa, Takashi Tani, Emily Y. Chu, José Luis Millán, et al. (2023). Dentoalveolar Alterations in an Adenine‐Induced Chronic Kidney Disease Mouse Model . Journal of Bone and Mineral Research.

Flávia Amadeu de Oliveira, Cintia K. Tokuhara, Vimal Veeriah, João Paulo Domezi, Mariana R. Santesso, Tania M. Cestari, Talita M.O. Ventura, Adriana A. Matos, Thiago Dionísio, Marcel R. Ferreira, et al. (2023). The Multifarious Functions of Leukotrienes in Bone Metabolism . Journal of Bone and Mineral Research.

Flavia Amadeu de Oliveira, Fatma F. Mohamed, Yuka Kinoshita, Sonoko Narisawa, Colin Farquharson, Koichi Miyake, Brian L Foster, Jose Luis Millan (2023). Gene Therapy Using Recombinant AAV Type 8 Vector Encoding TNAP‐D10 Improves the Skeletal Phenotypes in Murine Models of Osteomalacia . JBMR Plus.

Alberto Pasquarelli and Luiz Henrique Silva Andrilli and Mayt{\^{e}} Bolean and Claudio Reis Ferreira and Marcos Ant{\^{o}}nio Eufr{\'{a}}sio Cruz and Flavia Amadeu de Oliveira and Ana Paula Ramos and Jos{\'{e}} Luis Mill{\'{a}}n and Massimo Bottini and Pietro Ciancaglini(2022). Ultrasensitive Diamond Microelectrode Application in the Detection of Ca2+ Transport by AnnexinA5-Containing Nanostructured Liposomes . Biosensors. 12. (7). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 525. {MDPI} {AG}

Fatma F. Mohamed and Michael B. Chavez and Flavia Amadeu de Oliveira and Sonoko Narisawa and Colin Farquharson and Jos{\'{e}} Luis Mill{\'{a}}n and Brian L. Foster(2022). Perspective on Dentoalveolar Manifestations Resulting From PHOSPHO1 Loss-of-Function: A Form of Pseudohypophosphatasia? . Frontiers in Dental Medicine. 3. Frontiers Media {SA}

Mariana R. Santesso and Fl{\'{a}}via A. Oliveira and Cintia K. Tokuhara and Gabriela S. N. Oliveira and Fl{\'{a}}via M. Levy and L{\'{\i}}gia S. Antonio and Mar{\'{\i}}lia A. R. Buzalaf and Rodrigo C. Oliveira(2021). Fluoride effects on cell viability and ENaC expression in kidney epithelial cells . Toxicology Mechanisms and Methods. 31. (8). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 566--571. Informa {UK} Limited

Yuka Kinoshita and Fatma F Mohamed and Flavia Amadeu de Oliveira and Sonoko Narisawa and Koichi Miyake and Brian L Foster and Jos{\'{e}} Luis Mill{\'{a}}n(2021). Gene Therapy Using Adeno‐Associated Virus Serotype 8 Encoding TNAP‐D 10 Improves the Skeletal and Dentoalveolar Phenotypes in Alpl −/− Mice . Journal of Bone and Mineral Research. 36. (9). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 1835--1849. Wiley

Gabriela Silva Neubern de Oliveira, Cintia Kazuko Tokuhara, Flávia Amadeu de Oliveira, Márcia Sirlene Zardin Graeff, Zohaib Nisar Khan, Luiz Leonardo Saldanha, Anne Lígia Dokkedal, Rodrigo Cardoso de Oliveira (2021). Effects of Qualea grandiflora Extract on the Expression of MMP-14 and HIF-1α in Cultured Fibroblasts and Preosteoblasts . Brazilian Archives of Biology and Technology.

(2020). Surface roughness of titanium disks influences the adhesion, proliferation and differentiation of osteogenic properties derived from human . International Journal of Implant Dentistry.

(2020). Phosphatidylserine controls calcium phosphate nucleation and growth on lipid monolayers: A physicochemical understanding of matrix vesicle-driven biomineralization . Journal of Structural Biology.

(2020). Visualization of Mineral‐Targeted Alkaline Phosphatase Binding to Sites of Calcification In Vivo . Journal of Bone and Mineral Research.

Ana Carolina Cestari Bighetti, Tania Mary Cestari, Paula Sanches Santos, Ricardo Vinicius Nunes Arantes, Suelen Paini, Gerson Francisco Assis, Bruna Carolina Costa, Flávia Amadeu de Oliveira, Cintia Kazuko Tokuhara, Rodrigo Cardoso de Oliveira, et al. (2020). In vitro and in vivo assessment of CaP materials for bone regenerative therapy. The role of multinucleated giant cells/osteoclasts in bone regeneration . Journal of Biomedical Materials Research Part B: Applied Biomaterials.

(2020). Wettability and pre-osteoblastic behavior evaluations of a dense bovine hydroxyapatite ceramics . Journal of Oral Science.

(2019). An extract from Myracrodruon urundeuva inhibits matrix mineralization in human osteoblasts .

(2019). HOXA cluster gene expression during osteoblast differentiation involves epigenetic control .

(2019). In vitro and in vivo assessment of CaP materials for bone regenerative therapy. The role of multinucleated giant cells/osteoclasts in bone regeneration .

(2019). TiF4 and NaF varnishes induce low levels of apoptosis in murine and human fibroblasts through mitochondrial Bcl-2 family and death receptor signalling .

(2018). Isolation and characterization of progenitor cells from surgically created early healing alveolar defects in humans: A preliminary study .

(2018). The effect of fluoride on the structure, function, and proteome of intestinal epithelia .

(2017). The cytotoxic effect of TiF4 and NaF on fibroblasts is influenced by the experimental model, fluoride concentration and exposure time .

(2017). Low level laser therapy modulates viability, alkaline phosphatase and matrix metalloproteinase-2 activities of osteoblasts .

(2016). “Aroeira” (Myracrodruon urundeuva) methanol extract: the relationship between chemical compounds and cellular effects .

(2016). Low intensity lasers differently induce primary human osteoblast proliferation and differentiation .

(2014). Laser and light-emitting diode effects on pre-osteoblast growth and differentiation .

(2014). Anacardic Acid from Brazilian Cashew Nut Trees Reduces Dentine Erosion .

(2013). Laser Phototherapy at High Energy Densities Do Not Stimulate Pre-Osteoblast Growth and Differentiation .

PREPRINT

Luiz Henrique da Silva Andrilli, Cintia Kazuko Tokuhara, Larwsk Hayann Silva, Angie Puelles, Colin Farquharson, Ana Paula Ramos, Jose Luis Millán, Flavia Amadeu de Oliveira, Pietro Ciancaglini (2026). PHOSPHO1 deficiency delays osteogenic differentiation of osteoblasts, impairing mineralization and altering the protein composition of matrix vesicles .