プロフィール詳細

プロジェクトを作成

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Dr. Gustavo F.に依頼

Ireland

Biotechnology Research Consultant | Marine Bioactives, Microbial Analysis, Sustainable Product Development & Scientific

プロフィール概要

I am a biotechnology researcher and microbiologist with expertise in marine biotechnology, bioactive compounds, environmental biotechnology, sustainable bioprocessing, and applied R&D. I hold a PhD in Microbiology, Master's degrees in Biotechnology and Environmental Engineering, and undergraduate degrees in Biochemical Engineering and Environmental Management. Currently, I am a Postdoctoral Researcher at Technological University of the Shannon (Ireland), where I develop sustainable extraction technologies and investigate bioactive compounds from Irish seaweeds for health, cosmetic, and biotechnology applications. I have extensive experience collaborating with industry partners on innovation projects involving product development, microbial characterization, sequencing, analytical testing, environmental monitoring, and process optimization. My technical expertise includes microbiology, molecular biology, immunology, fermentation, bioactive compound extraction, 16S rRNA sequencing, LC-MS analysis, experimental design, data analysis, scientific writing, and project management. I have contributed to numerous peer-reviewed publications and have supervised PhD, Master's, and undergraduate research projects. I am available for consulting projects involving biotechnology, microbiology, environmental science, literature reviews, scientific writing, grant applications, experimental design, data analysis, product validation, and research commercialization. I help clients transform scientific challenges into practical, evidence-based solutions that support innovation and business growth.

専門分野

• ☆ Marine Chemistry
• ☆ Inorganic Chemistry
• ☆ Molecular Engineering
• ☆ Electrochemistry
• ☆ Agricultural Chemistry
• ☆ Flow Chemistry
• ☆ Green Chemistry
• ☆ Microbiology
• ☆ Microbiome Research
• ☆ Phycology/Algae
• ☆ Mycology/Fungi
• ☆ Industrial Microbiology
• ☆ Microbiological Assays
• ☆ Agricultural Microbiology
• ☆ Biostatistics
• ☆ Biochemistry
• ☆ Immunoassay
• ☆ Biochemical Assays
• ☆ Oxidative Stress & Mitochondrial Function
• ☆ Antioxidants
• ☆ Enzyme Activity Assays
• ☆ Molecular Biology
• ☆ Biomarkers
• ☆ Genetics
• ☆ PCR
• ☆ Next Generation Sequencing (NGS)
• ☆ DNA Sequencing
• ☆ Gene Expression Analysis
• ☆ Genome Sequencing
• ☆ Molecular Ecology
• ☆ Biotechnology & Bioengineering
• ☆ Microscopy
• ☆ Light Microscopy
• ☆ Fluorescence Microscopy
• ☆ Environmental Science
• ☆ Environmental Health
• ☆ Food Engineering
• ☆ Nutrition Science
• ☆ Food Microbiology
• ☆ Food Safety
• ☆ Dairy Science
• ☆ Food Chemistry
• ☆ Nutritional Analysis
• ☆ Clinical Microbiology
• ☆ Applied Chemistry
• ☆ Biomarker Discovery
• ☆ Agricultural Biotechnology
• ☆ Tissue Engineering
• ☆ Drug Development
• ☆ Biosafety Testing
• ☆ Microbiology Testing
• ☆ Bioanalytical Testing
• ☆ Drug Stability Testing
• ☆ In vitro Preclinical Toxciology
• ☆ Product Evaluation
• ☆ Reverse Engineering
• ☆ Hypothesis
• ☆ Peer Review
• ☆ Research Methodology
• ☆ Feasibility Studies
• ☆ Technology Scouting
• ☆ Fact Checking
• ☆ Literature Search
• ☆ Gap Analysis
• ☆ Technical & Scientific Writing
• ☆ Journal Selection
• ☆ Manuscript & Journal Article Writing
• ☆ Instructional Materials
• ☆ Release Notes
• ☆ Experimental Protocols
• ☆ SOP & Protocol Writing
• ☆ Case Studies
• ☆ Science Communication
• ☆ Literature Review
• ☆ Traditional/Narrative Literature Review
• ☆ Systematic Literature Review
• ☆ Cosmetic & Skincare Formulation
• ☆ Herbal Products
• ☆ Nutraceuticals
• ☆ Essential Oils

サービス

• Writing
• Research
• Consulting
• Data & AI
• Product Development

Writing Non-Medical Regulatory Writing, Technical Writing, General Proofreading & Editing

Research Market Research, Meta-Research, Feasibility Study, Fact Checking, Gap Analysis, Gray Literature Search, Scientific and Technical Research, Systematic Literature Review

Consulting Business Strategy Consulting, Go-to-Market Strategy Consulting, Scientific and Technical Consulting, Regulatory Consulting, Manufacturing Consulting

Data & AI Predictive Modeling, Statistical Analysis, Image Processing, Image Analysis, Data Visualization, Data Mining, Data Cleaning, Data Insights

Product Development Formulation, Recipe Development, Stability/Shelf Life Testing, Product Validation, Manufacturing, Quality Assurance & Control (QA/QC), Concept Development, Device Fabrication

職務経験

Researcher

TUS

- 現在

Assistant Lecturer

Technological University of the Shannon

2月 2024 - 現在

Research Scientist

Technological University of the Shannon: Midwest

10月 2024 - 1月 2025

Doctoral researcher/Research Assistant

CABR

4月 2022 - 8月 2024

QUALITY ASSURANCE QUALITY CONTROL

BOM PRATO REFEIÇÕES

9月 2019 - 12月 2019

Intern

EMBRAPA

10月 2016 - 12月 2016

学歴

Post graduation in Engineering and Environmental Science

Instituto Federal Sul-rio-grandense

7月 2017 - 8月 2020

Post graduation in Biotechnology (Biotechnology)

Universidade Federal de Pelotas

1月 2017 - 2月 2019

Bachelor in Biochemical Engineering

Universidade Federal do Rio Grande

3月 2010 - 1月 2017

Technologist in Environmental Management

Instituto Federal Sul-rio-grandense

6月 2009 - 8月 2014

認定資格

• Attune Cytpix Flow Cytometer

  Thermofisher

  9月 2025 - 現在

• Organ-on-chip

  Emulate

  12月 2024 - 現在

• PCR and qPCR

  Thermofisher

  6月 2024 - 現在

出版物

JOURNAL ARTICLE

Mariana J. Silva, Gustavo W. Fehrenbach, Robert Pogue, Patrick Murray, Emanuele Rezoagli, John G. Laffey, Emma J. Murphy (2026). Sepsis modelling: current approaches and organ-on-chip perspectives . eBioMedicine.

Sepsis modelling: current approaches and organ-on-chip perspectives @article { author = {Mariana J. Silva and Gustavo W. Fehrenbach and Robert Pogue and Patrick Murray and Emanuele Rezoagli and John G. Laffey and Emma J. Murphy}, title = {Sepsis modelling: current approaches and organ-on-chip perspectives}, journal = {eBioMedicine}, year = {2026}, month = {mar}, url = {http://dx.doi.org/10.1016/j.ebiom.2026.106120}, doi = {10.1016/j.ebiom.2026.106120} } . eBioMedicine.

Controlled-release hybrid hydrogels delivering synergistic N-acetyl cysteine, ibuprofen, and progesterone combinations for enhanced peripheral nerve regeneration @article{444448dab970481288abe51f0910ca94, title = "Controlled-release hybrid hydrogels delivering synergistic N-acetyl cysteine, ibuprofen, and progesterone combinations for enhanced peripheral nerve regeneration", abstract = "Peripheral nerve injuries (PNI) remain a major clinical challenge, particularly for defects exceeding 3 cm. Autografts are the current gold standard for small gaps, but their use is limited by donor site morbidity, infection risk, and poor outcomes in larger defects. Synthetic grafts and allografts have achieved only modest clinical success, underscoring the need for therapeutic systems that actively support regeneration. This study investigated three FDA-approved drugs − N-acetyl cysteine (NAC), ibuprofen (Ibu), and progesterone (Prog) − for their synergistic potential to enhance Schwann cell (SC) and PC-12 cell proliferation as a strategy for peripheral nerve repair. High-throughput screening using Alamar Blue assays assessed viability and proliferation at 24, 48, and 72 h across dual- and triple-drug combinations. The optimised formulation of 75 µM NAC, 16.25 µM Ibu, and 30 µM Prog significantly increased proliferation compared with untreated controls without inducing cytotoxicity. A validated high-performance liquid chromatography (HPLC) method was developed to quantify simultaneous release of all three drugs from polyethylene glycol dimethacrylate–dipentaerythritol hexa(3-mercaptopropionate) (PEGDMA–DiPETMP) hydrogels. Controlled, sequential release was achieved, with complete elution of NAC within 24 h, Ibu within 72 h, and Prog over 21 days. These timelines correspond to the early antioxidative, anti-inflammatory, and neurotrophic phases of peripheral nerve repair. Although proliferation assays were conducted using directly applied drug combinations, the established release behaviour provides a mechanistic rationale for future validation of hydrogel-mediated delivery. The synergistic combination of NAC, Ibu, and Prog delivered from PEGDMA–DiPETMP hybrid hydrogels provides a reproducible and temporally coordinated release platform that promotes cellular proliferation and supports peripheral nerve regeneration.", keywords = "Controlled drug release, High-performance liquid chromatography (HPLC), Hybrid hydrogels, PC12 cells, Peripheral nerve repair, Schwann cells, Synergistic drug combinations", author = "Gavin Burke and Farnoosh Ebrahimi and Gustavo Fehrenbach and Ciara Buckley and Emma Murphy and Declan Devine and Ian Major", note = "Publisher Copyright: {\textcopyright} 2025 Elsevier B.V.", year = "2026", month = jan, day = "5", doi = "10.1016/j.ijpharm.2025.126423", language = "English", volume = "687", journal = "International Journal of Pharmaceutics", issn = "0378-5173", publisher = "Elsevier B.V.", } . International Journal of Pharmaceutics.

Gustavo Fehrenbach, Gavin Burke, Farnoosh Ebrahimi, Ciara Buckley, Emma Murphy, Declan Devine, Ian Major (2026). Controlled-release hybrid hydrogels delivering synergistic N-acetyl cysteine, ibuprofen, and progesterone combinations for enhanced peripheral nerve regeneration . International Journal of Pharmaceutics.

Controlled-release hybrid hydrogels delivering synergistic N-acetyl cysteine, ibuprofen, and progesterone combinations for enhanced peripheral nerve regeneration @article { author = {Gavin Burke and Farnoosh Ebrahimi and Gustavo Fehrenbach and Ciara Buckley and Emma Murphy and Declan Devine and Ian Major}, title = {Controlled-release hybrid hydrogels delivering synergistic N-acetyl cysteine, ibuprofen, and progesterone combinations for enhanced peripheral nerve regeneration}, journal = {International Journal of Pharmaceutics}, year = {2026}, month = {jan}, url = {https://doi.org/10.1016/j.ijpharm.2025.126423}, doi = {10.1016/j.ijpharm.2025.126423} } . International Journal of Pharmaceutics.

Fabrication of continuous iron wire reinforced poly-L-lactic acid (PLLA) filaments for producing biodegradable stents via a braiding process @article{c7fba05442364492bd11e844d9d964f9, title = "Fabrication of continuous iron wire reinforced poly-L-lactic acid (PLLA) filaments for producing biodegradable stents via a braiding process", abstract = "Poly-L-lactic acid (PLLA) is widely used in medical applications due to its excellent biodegradability and biocompatibility. However, its limited mechanical strength poses challenges for applications like biodegradable stents, which require both strength and flexibility. In this study, continuous iron wire reinforced PLLA (CIWR-PLLA) filaments were fabricated using crosshead extrusion to enhance the mechanical properties of PLLA. The tensile strength and Young's modulus of CIWR-PLLA filaments were significantly improved to 69.3 ± 1.12 MPa and 3.4 ± 0.17 GPa, respectively, compared to 36.7 ± 1.21 MPa and 2.6 ± 0.03 GPa for pure PLLA. Stents braided from CIWR-PLLA filaments exhibited nearly double the radial compression force compared to those made from pure PLLA, demonstrating superior mechanical performance. Cytotoxicity assessments using Caco-2 cells confirmed that both PLLA and CIWR-PLLA are non-toxic and biocompatible. The immunological study demonstrates that CIWR-PLLA exhibits excellent biocompatibility, eliciting minimal macrophage activation and only mild epithelial cytokine response, confirming its suitability for implantable medical applications. Accelerated degradation studies conducted at 50°C revealed that CIWR-PLLA maintained structural integrity longer than PLLA due to the reinforcing effect of the iron wire. Significant morphological changes and mass reduction were observed from week 6 onward, indicating this as a critical degradation point, equivalent to approximately six months at 37°C. These results suggest that CIWR-PLLA filaments offer improved mechanical performance, biocompatibility, and controlled degradation, making them promising candidates for next-generation biodegradable stent application.", keywords = "Bioresorbable scaffolds, Braiding, Continuous iron wire reinforced filament, Poly-L-lactic acid", author = "Yuanyuan Chen and Handai Liu and Murphy, \{Emma J.\} and Fehrenbach, \{Gustavo Waltzer\} and \{De Lima\}, \{Tielidy Angelina De Morais\} and Dhanyamol George and Niall Burke and Aiden Flannagan", note = "Publisher Copyright: {\textcopyright} 2025 The Author(s)", year = "2025", month = dec, doi = "10.1016/j.rineng.2025.107458", language = "English", volume = "28", journal = "Results in Engineering", issn = "2590-1230", publisher = "Elsevier B.V.", } . Results in Engineering.

Yuanyuan Chen, Handai Liu, Emma J. Murphy, Gustavo Waltzer Fehrenbach, Tielidy Angelina De Morais De Lima, Dhanyamol George, Niall Burke, Aiden Flanagan (2025). Fabrication of continuous iron wire reinforced poly-L-lactic acid (PLLA) filaments for producing biodegradable stents via a braiding process . Results in Engineering.

Fabrication of continuous iron wire reinforced poly-L-lactic acid (PLLA) filaments for producing biodegradable stents via a braiding process @article { author = {Yuanyuan Chen and Handai Liu and Emma J. Murphy and Gustavo Waltzer Fehrenbach and Tielidy Angelina De Morais De Lima and Dhanyamol George and Niall Burke and Aiden Flanagan}, title = {Fabrication of continuous iron wire reinforced poly-L-lactic acid (PLLA) filaments for producing biodegradable stents via a braiding process}, journal = {Results in Engineering}, year = {2025}, month = {dec}, url = {https://doi.org/10.1016/j.rineng.2025.107458}, doi = {10.1016/j.rineng.2025.107458} } . Results in Engineering.

Gustavo Waltzer Fehrenbach, João Rui Tanoeiro, Robert Pogue, Frank Carter, Marco F.L. Lemos, Patrick Murray, Declan Devine, Emanuele Rezoagli, Ian Major, Emma J. Murphy (2025). Beta-glucan-enriched diets improve immune function, antioxidant activity, and survivability in challenged oysters . Food Hydrocolloids for Health.

Beta-glucan-enriched diets improve immune function, antioxidant activity, and survivability in challenged oysters @article{4641a1a7924e450e89968a5e7a0f0e6d, title = "Beta-glucan-enriched diets improve immune function, antioxidant activity, and survivability in challenged oysters", abstract = "Beta-glucans are bioactive polysaccharides with immunomodulatory and hydrocolloid properties. This study explores the potential of beta-glucans extracted from mushroom waste to enhance oyster (Crassostrea gigas) immunity and quality, providing a sustainable alternative to conventional aquaculture interventions. Beta-glucan supplementation led to a 100 \% survival rate in treated groups compared to 70 \% in the bacteria-only group (p = 0.024). Flow cytometry revealed a significant reduction in viable hemocytes in the beta-glucan group post-infection, suggesting modulation of inflammation. Protein content in gills, mantle, and digestive tissues increased by up to 2-fold in beta-glucan-fed oysters versus controls. Antioxidant activity, assessed by FRAP assay, was 25–40 \% higher in the mantle and digestive tract if beta-glucan groups. Bacteriostatic assays demonstrated a marked reduction in Vibrio sp. Counts in tissues, particularly in the digestive tract, with bacterial load similar to uninfected controls. These findings highlight the dual functionality of beta-glucans as both immunomodulators and hydrocolloids, with implications for improving aquaculture sustainability and food safety. Further research should explore feeding behaviour, gut transit time, and nutrient absorption to optimize beta-glucan application in commercial shellfish production.", keywords = "Aquaculture, Beta-glucan, Ecological sustainability, Environmental contaminants, Food safety, Immune response, Sustainable practices", author = "Fehrenbach, \{Gustavo Waltzer\} and Tanoeiro, \{Jo{\~a}o Rui\} and Robert Pogue and Frank Carter and Lemos, \{Marco F.L.\} and Patrick Murray and Declan Devine and Emanuele Rezoagli and Ian Major and Murphy, \{Emma J.\}", note = "Publisher Copyright: {\textcopyright} 2025 The Authors", year = "2025", month = dec, doi = "10.1016/j.fhfh.2025.100227", language = "English", volume = "8", journal = "Food Hydrocolloids for Health", issn = "2667-0259", publisher = "Elsevier B.V.", } . Food Hydrocolloids for Health.

Antibacterial Potential of Essential Oils Against E. coli and Salmonella spp. in Minimally Processed Foods @article{7d15121340304ef28a70ac804298b817, title = "Antibacterial Potential of Essential Oils Against E. coli and Salmonella spp. in Minimally Processed Foods", abstract = "Minimally processed foods (MPFs), often considered ready-to-eat, do not undergo cooking and therefore require proper handling and preparation to ensure safety. If not handled correctly, these foods can serve as a pathway for diseases caused by pathogenic bacteria, including Escherichia coli and Salmonella spp. The antibacterial activity of essential oils (EOs) has been increasingly studied as a tool for controlling microorganisms in the food sector. Therefore, we aimed to verify the contamination of MPF by E. coli and Salmonella and to test the sensitivity of these strains to Copaifera langsdorffii, Schinus terebinthifolius, Citrus reticulata, Eucalyptus citriodora, Elettaria cardamomum, Ocimum basilicum, and Eugenia caryophyllus EOs using the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) methods. From 25 MPF samples, one E. coli strain and one Salmonella spp. were isolated. C. langsdorffii and C. reticulata EOs did not show antibacterial activity, while S. terebinthifolius and E. citriodora inhibited the growth of both strains. The E. cardamomum, O. basilicum, and E. caryophyllus EOs presented inhibitory and bactericidal responses at concentrations 0.78, 0.39, and 0.19\% (v/v), respectively, compared to the two isolated strains. The present study reinforces the antibacterial potential of EOs and suggests their application in the MPF production chain.", keywords = "E. coli, essential oil, foodborne disease, MIC, ready-to-eat, Salmonella", author = "Aline Sitowski and Ribeiro, \{Gladis Aver\} and Murphy, \{Emma J.\} and Fehrenbach, \{Gustavo Waltzer\}", note = "Publisher Copyright: {\textcopyright} 2025 by the authors.", year = "2025", month = jun, doi = "10.3390/bacteria4020020", language = "English", volume = "4", journal = "Bacteria", issn = "2674-1334", publisher = "Multidisciplinary Digital Publishing Institute (MDPI)", number = "2", } . Bacteria.

Anti-Inflammatory and Regenerative Properties of Herbal Extracts: Wound Management in Equine Models @article { author = {Gustavo Fehrenbach and Katie Shiels and Mariana Juca Silva and Jessica Walshe and Lena Madden and Ian Major and Niall Burke and Tim Yeomans and Emanuele Rezoagli and Emma Murphy}, title = {Anti-Inflammatory and Regenerative Properties of Herbal Extracts: Wound Management in Equine Models}, journal = {Drug Target Insights}, year = {2025}, month = {may}, url = {https://doi.org/10.33393/dti.2025.3499}, doi = {10.33393/dti.2025.3499} } . Drug Target Insights.

Gustavo Fehrenbach, Katie Shiels, Mariana Juca Silva, Jessica Walshe, Lena Madden, Ian Major, Niall Burke, Tim Yeomans, Emanuele Rezoagli, Emma Murphy (2025). Anti-Inflammatory and Regenerative Properties of Herbal Extracts: Wound Management in Equine Models . Drug Target Insights.

Monitoring water contamination through shellfish @article{894ffc4e885e4c30911da5ea5e869015, title = "Monitoring water contamination through shellfish: A systematic review of biomarkers, species selection, and host response", abstract = "Shellfish are ubiquitous inhabitants of water environments and an important food source. Their presence in habitats affected by human activities and bioaccumulation potential support their use as indicators of contamination. Using these organisms as bioindicators may provide a simple, cost-effective alternative for monitoring contamination risk without requiring complex sample preparation and analytical procedures. However, there is no consensus on biomarkers, species selection, or standard methodologies. This systematic review explores the significance of shellfish as bioindicators, the methodologies employed, and the interactions between contaminants and host response. Research strategy and selection criteria followed PRISMA and SPICE guidelines. Peer-reviewed papers were obtained from Scopus, Pubmed and Google Scholar using the keywords: shellfish, bioindicator, environmental, contaminant, and host response. Out of 3050 results, 20 studies were selected after a rigorous, unbiased process. These studies spanned 14 countries, assessing 26 shellfish species, 24 contaminants, and 35 effects. A wide range of biomarkers was identified, reflecting changes in water condition due to contaminants or adverse environmental parameters. Results indicate that shellfish are highly sensitive to minor environmental changes, making them reliable bioindicators of water quality and potential risks of consumption from contaminated sites. This study highlights the need for standardized protocols to enable consistent shellfish-based monitoring of water conditions worldwide.", keywords = "Bacteria, Environmental contaminants, Heavy metals, Host response, Microplastics, Pesticides pharmaceuticals", author = "Fehrenbach, \{Gustavo Waltzer\} and Emma Murphy and Tanoeiro, \{Jo{\~a}o Rui\} and Robert Pogue and Ian Major", note = "Publisher Copyright: {\textcopyright} 2025 The Authors", year = "2025", month = apr, day = "15", doi = "10.1016/j.ecoenv.2025.118120", language = "English", volume = "295", journal = "Ecotoxicology and Environmental Safety", issn = "0147-6513", publisher = "Academic Press", } . Ecotoxicology and Environmental Safety.

Aline Sitowski, Gladis Aver Ribeiro, Emma J. Murphy, Gustavo Waltzer Fehrenbach (2025). Antibacterial Potential of Essential Oils Against E. coli and Salmonella spp. in Minimally Processed Foods . Bacteria.

Antibacterial Potential of Essential Oils Against E. coli and Salmonella spp. in Minimally Processed Foods @article { author = {Aline Sitowski and Gladis Aver Ribeiro and Emma J. Murphy and Gustavo Waltzer Fehrenbach}, title = {Antibacterial Potential of Essential Oils Against E. coli and Salmonella spp. in Minimally Processed Foods}, journal = {Bacteria}, year = {2025}, month = {apr}, url = {https://doi.org/10.3390/bacteria4020020}, doi = {10.3390/bacteria4020020} } . Bacteria.

Aline Sitowski, Gladis Aver Ribeiro, Emma J. Murphy, Gustavo Waltzer Fehrenbach (2025). Antibacterial Potential of Essential Oils Against <i>E. coli</i> and <i>Salmonella</i> spp. in Minimally Processed Foods . Bacteria.

Gustavo Waltzer Fehrenbach, Emma Murphy, Jo&#227;o Rui Tanoeiro, Robert Pogue, Ian Major (2025). Monitoring water contamination through shellfish: A systematic review of biomarkers, species selection, and host response . Ecotoxicology and Environmental Safety.

Livestock Slurry and Sustainable Pasture Management @article{701efa9d04584fcfbad256d425ad3eaa, title = "Livestock Slurry and Sustainable Pasture Management: Microbial Roles, Environmental Impacts, and Regulatory Perspectives in Ireland and Europe", abstract = "Pastures serve as the primary source of grass and forage plants for grazing livestock, requiring adequate nutrient input to sustain growth and soil fertility. Slurry from the livestock industry is widely utilized as a sustainable and cost-effective alternative to chemical fertilizers. Microorganisms within the slurry–pasture system are essential for breaking down organic matter, facilitating nutrient cycling, and improving soil health. However, mismanagement or inefficient microbial decomposition can lead to significant issues, such as nutrient leaching into water bodies, causing eutrophication, antimicrobial resistance, and reduced nutrient availability in pastures, which, in turn, may negatively impact livestock productivity. Thus, this paper investigates the composition and benefits of livestock slurry in pasture management, highlights microbial roles in nutrient cycling, and evaluates regulatory frameworks in Ireland and Europe. Additionally, it examines the environmental risks associated with improper slurry application, providing insights to support sustainable management practices.", keywords = "antimicrobial resistance, carbon emissions, microorganisms, nutrient leaching, slurry fertilization", author = "Silva, \{Mariana Juca\} and Ismin Zainol and Tanoeiro, \{Jo{\~a}o Rui\} and Aline Sitowski and Ian Major and Murphy, \{Emma J.\} and Fehrenbach, \{Gustavo Waltzer\}", note = "Publisher Copyright: {\textcopyright} 2025 by the authors.", year = "2025", month = apr, doi = "10.3390/microorganisms13040788", language = "English", volume = "13", journal = "Microorganisms", issn = "2076-2607", publisher = "Multidisciplinary Digital Publishing Institute (MDPI)", number = "4", } . Microorganisms.

Livestock Slurry and Sustainable Pasture Management: Microbial Roles, Environmental Impacts, and Regulatory Perspectives in Ireland and Europe @article { author = {Mariana Juca Silva and Ismin Zainol and João Rui Tanoeiro and Aline Sitowski and Ian Major and Emma J. Murphy and Gustavo Waltzer Fehrenbach}, title = {Livestock Slurry and Sustainable Pasture Management: Microbial Roles, Environmental Impacts, and Regulatory Perspectives in Ireland and Europe}, journal = {Microorganisms}, year = {2025}, month = {mar}, url = {https://doi.org/10.3390/microorganisms13040788}, doi = {10.3390/microorganisms13040788} } . Microorganisms.

Mariana Juca Silva, Ismin Zainol, Jo&#227;o Rui Tanoeiro, Aline Sitowski, Ian Major, Emma J. Murphy, Gustavo Waltzer Fehrenbach (2025). Livestock Slurry and Sustainable Pasture Management: Microbial Roles, Environmental Impacts, and Regulatory Perspectives in Ireland and Europe . Microorganisms.

Mariana Juca Silva, Ismin Zainol, Jo&#227;o Rui Tanoeiro, Aline Sitowski, Ian Major, Emma J. Murphy, Gustavo Waltzer Fehrenbach (2025). Livestock Slurry and Sustainable Pasture Management: Microbial Roles, Environmental Impacts, and Regulatory Perspectives in Ireland and Europe . Microorganisms.

Exploring the role of polysaccharides from macroalgae in shaping gut microbiome and promoting anti-obesity outcomes @article{0fc926ebc2c1492586218948f5b9702a, title = "Exploring the role of polysaccharides from macroalgae in shaping gut microbiome and promoting anti-obesity outcomes", abstract = "Obesity rates have risen significantly globally, yet effective and safe drug treatments remain elusive. As researchers explore alternative solutions to address this growing epidemic, macroalgae have gained attention due to their unique polysaccharides. Recent studies have highlighted the complex interactions between dietary polysaccharides and gut flora, suggesting that these carbohydrates can profoundly influence the microbial community structure and function. By focusing on the mechanisms through which these polysaccharides influence metabolic processes, this paper explores how they contribute to anti-obesity effects, such as reducing inflammation, enhancing satiety, and improving lipid metabolism. The review synthesizes findings from both in vivo and in vitro studies to present a comprehensive overview of how macroalgal polysaccharides facilitate the growth of beneficial gut bacteria, which in turn produce metabolites like short-chain fatty acids (SCFAs). These metabolites play crucial roles in energy homeostasis, immune modulation, and overall host health. However, to fully understand how these elements contribute to maintaining gut balance and overall health, more research through animal studies and human clinical trials is needed. Through this review, we aim to provide insights that could lead to more targeted and effective use of macroalgae as a functional food component in obesity management and prevention strategies.", keywords = "Gut microbiome, Immune modulation, Intestinal health, Macroalgae, Obesity, Polysaccharides", author = "\{Amiri Goushki\}, Mehdi and Zahra Kharat and Murphy, \{Emma J.\} and Declan Devine and Noel Gately and \{Fuenmayor Antunez\}, Evert and Gustavo Fehrenbach", note = "Publisher Copyright: {\textcopyright} 2024", year = "2025", month = mar, doi = "10.1016/j.foodhyd.2024.110758", language = "English", volume = "160", journal = "Food Hydrocolloids", issn = "0268-005X", publisher = "Elsevier B.V.", } . Food Hydrocolloids.

Mehdi Amiri Goushki, Zahra Kharat, Gustavo Waltzer Fehrenbach, Emma J. Murphy, Declan Devine, Noel Gately, Evert Fuemayor (2025). Exploring the role of polysaccharides from macroalgae in shaping gut microbiome and promoting anti-obesity outcomes . Food Hydrocolloids.

Anti-Inflammatory and Regenerative Properties of Herbal Extracts @article{0391de06493b47e3acea0ffe1a5f0559, title = "Anti-Inflammatory and Regenerative Properties of Herbal Extracts: Wound Management in Equine Models", abstract = "Introduction: Wound management presents significant challenges, requiring effective treatments. Herbal extracts have been traditionally used to support healing due to their anti-inflammatory, antimicrobial, and cellregenerative properties. Methods: This study aimed to evaluate the therapeutic efficacy of Pau D{\textquoteright}Arco (Tabebuia), Yarrow (Achillea millefolium), Gotu Kola (Centella asiatica), Figwort (Scrophularia nodosa), and Broadleaf (Plantago major) extracts, both individually and combined, on wound healing in vitro and in vivo in equine models. In vitro tests using human macrophages and keratinocyte cell lines to assess cellular responses such as cytokine secretion and phagocytic activity under simulated inflammatory conditions. Additionally, pilot case studies on equines with open wounds provided practical insights into the extracts{\textquoteright} healing capabilities. Results: MTT assay was used to assess cytotoxicity. The extracts did not significantly affect the viability of HaCaT or THP-1 cells. The herbal extracts reduced IL-8 levels and increased phagocytic activity in macrophages, indicating an ability to modulate inflammatory responses. In vivo, the extracts were well tolerated and associated with supported healing in equines. These effects were suggested to be attributed to the synergistic actions of the herbal components. Conclusion: These findings suggest that the herbal extracts may be useful for supporting wound healing. Their natural anti-inflammatory and healing properties could provide an additional option alongside traditional wound management approaches.", keywords = "Anti-inflammatory agents, Herbal extracts, Natural compounds, Synergistic effects, Tissue regetion, Wound healing", author = "Fehrenbach, \{Gustavo W.\} and Katie Sheils and Silva, \{Mariana J.\} and Jessica Walshe and Lena Madden and Ian Major and Niall Burke and Tim Yeomans and Emanuele Rezoagli and Murphy, \{Emma J.\}", note = "Publisher Copyright: {\textcopyright} 2025 The Authors.", year = "2025", month = jan, day = "1", doi = "10.33393/dti.2025.3499", language = "English", volume = "19", pages = "41--48", journal = "Drug Target Insights", issn = "1177-3928", publisher = "AboutScience Srl", number = "1", } . Drug Target Insights.

Sitowski, A., Ribeiro, G.A., Murphy, E.J., Fehrenbach, G.W.(2025). Antibacterial Potential of Essential Oils Against E. coli and Salmonella spp. in Minimally Processed Foods . Bacteria. 4. (2).

Silva, M.J., Zainol, I., Tanoeiro, J.R., Sitowski, A., Major, I., Murphy, E.J., Fehrenbach, G.W.(2025). Livestock Slurry and Sustainable Pasture Management: Microbial Roles, Environmental Impacts, and Regulatory Perspectives in Ireland and Europe . Microorganisms. 13. (4).

Fehrenbach, G.W., Sheils, K., Silva, M.J., Walshe, J., Madden, L., Major, I., Burke, N., Yeomans, T., Rezoagli, E., Murphy, E.J.(2025). Anti-Inflammatory and Regenerative Properties of Herbal Extracts: Wound Management in Equine Models . Drug Target Insights. 19. (1). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 41-48.

Fehrenbach, G.W., Tanoeiro, J.R., Pogue, R., Carter, F., Lemos, M.F.L., Murray, P., Devine, D., Rezoagli, E., Major, I., Murphy, E.J.(2025). Beta-glucan-enriched diets improve immune function, antioxidant activity, and survivability in challenged oysters . Food Hydrocolloids for Health. 8.

Goushki, M.A., Kharat, Z., Fehrenbach, G.W., Murphy, E.J., Devine, D., Gately, N., Fuemayor, E.(2025). Exploring the role of polysaccharides from macroalgae in shaping gut microbiome and promoting anti-obesity outcomes . Food Hydrocolloids. 160.

Fehrenbach, G.W., Murphy, E., Tanoeiro, J.R., Pogue, R., Major, I.(2025). Monitoring water contamination through shellfish: A systematic review of biomarkers, species selection, and host response . Ecotoxicology and Environmental Safety. 295.

Exploring the role of polysaccharides from macroalgae in shaping gut microbiome and promoting anti-obesity outcomes @article { author = {Goushki, M.A. and Kharat, Z. and Fehrenbach, G.W. and Murphy, E.J. and Devine, D. and Gately, N. and Fuemayor, E.}, title = {Exploring the role of polysaccharides from macroalgae in shaping gut microbiome and promoting anti-obesity outcomes}, journal = {Food Hydrocolloids}, volume = {160}, year = {2025}, url = {https://www.scopus.com/inward/record.url?eid=2-s2.0-85206978878&partnerID=MN8TOARS}, doi = {10.1016/j.foodhyd.2024.110758} } . Food Hydrocolloids.

Beta-glucan-enriched diets improve immune function, antioxidant activity, and survivability in challenged oysters @article { author = {Fehrenbach, G.W. and Tanoeiro, J.R. and Pogue, R. and Carter, F. and Lemos, M.F.L. and Murray, P. and Devine, D. and Rezoagli, E. and Major, I. and Murphy, E.J.}, title = {Beta-glucan-enriched diets improve immune function, antioxidant activity, and survivability in challenged oysters}, journal = {Food Hydrocolloids for Health}, volume = {8}, year = {2025}, url = {https://www.scopus.com/inward/record.url?eid=2-s2.0-105008531214&partnerID=MN8TOARS}, doi = {10.1016/j.fhfh.2025.100227} } . Food Hydrocolloids for Health.

Monitoring water contamination through shellfish: A systematic review of biomarkers, species selection, and host response @article { author = {Fehrenbach, G.W. and Murphy, E. and Tanoeiro, J.R. and Pogue, R. and Major, I.}, title = {Monitoring water contamination through shellfish: A systematic review of biomarkers, species selection, and host response}, journal = {Ecotoxicology and Environmental Safety}, volume = {295}, year = {2025}, url = {https://www.scopus.com/inward/record.url?eid=2-s2.0-105001869483&partnerID=MN8TOARS}, doi = {10.1016/j.ecoenv.2025.118120} } . Ecotoxicology and Environmental Safety.

Tielidy A. de M. de Lima, Gabriel Goetten de Lima, Pierre Rouxel, Gilberto Silva Nunes Bezerra, Gustavo W. Fehrenbach, Washington L.E. Magalh&#227;es, Michael J.D. Nugent (2024). Extraction and characterization of microfibrillated cellulose (MFC) from Rhododendron ponticum isolated using cryocrush pre-treatment and its potential for mycelium cultivation . International Journal of Biological Macromolecules.

Extraction and characterization of microfibrillated cellulose (MFC) from Rhododendron ponticum isolated using cryocrush pre-treatment and its potential for mycelium cultivation @article{db48452e92734ffd8bc60be29a806281, title = "Extraction and characterization of microfibrillated cellulose (MFC) from Rhododendron ponticum isolated using cryocrush pre-treatment and its potential for mycelium cultivation", abstract = "Rhododendron ponticum (R. ponticum), a rapidly spreading invasive species in Ireland, was investigated for its potential use in creating sustainable bioproducts. This study explored the utilization of R. ponticum biomass as a source of microfibrillated cellulose (MFC) for fungal cultivation. The production of MFC was evaluated employing a novel cryocrushing treatment combined with a twin-screw extruder (TSE). The results demonstrated a significant increase in film strength, up to 332.3 MPa, with increasing TSE steps compared to 72.5 MPa in untreated samples. X-ray diffraction (XRD) analysis revealed a decrease in crystallinity from 68.93 \% to 59.2 \%, following cryocrushing and TSE treatment. Additionally, MFC subjected to the highest TSE treatment (12 steps) was successfully used as a substrate for cultivating Agaricus blazei mushrooms using 0.2 wt\%, 0.5 wt\%, and 1 wt\% MFC over a period of 7 days. Fourier-transform infrared spectroscopy (FTIR) confirmed the presence of chitin/chitin glucan within the fungal fibers. This research highlights the potential for transforming the invasive R. ponticum into valuable biocomposite materials. These MFC-fungus composites hold promise for various applications, including sustainable packaging, biodegradable plastics, and eco-friendly textiles.", keywords = "Cryocrush, Fungal cultivation, Natural fibers", author = "Tielidy, \{Tielidy A.\} and \{de Lima\}, \{Gabriel Goetten\} and Pierre Rouxel and Bezerra, \{Gilberto Silva Nunes\} and Fehrenbach, \{Gustavo W.\} and Magalh{\~a}es, \{Washington L.E.\} and Nugent, \{Michael J.D.\}", note = "Publisher Copyright: {\textcopyright} 2024", year = "2024", month = nov, doi = "10.1016/j.ijbiomac.2024.135284", language = "English", volume = "279", journal = "International Journal of Biological Macromolecules", issn = "0141-8130", publisher = "Elsevier B.V.", } . International Journal of Biological Macromolecules.

Gustavo Fehrenbach, Keran Zhou, Bianca Simonassi-Paiva, Guangming Yan, Alexandre Portela, Robert Pogue, Zhi Cao, Margaret Brennan Fournet, Declan M. Devine (2024). Investigating the Promising P28 Peptide-Loaded Chitosan/Ceramic Bone Scaffolds for Bone Regeneration . Molecules.

Investigating the Promising P28 Peptide-Loaded Chitosan/Ceramic Bone Scaffolds for Bone Regeneration @article { author = {Keran Zhou and Bianca Simonassi-Paiva and Gustavo Fehrenbach and Guangming Yan and Alexandre Portela and Robert Pogue and Zhi Cao and Margaret Brennan Fournet and Declan M. Devine}, title = {Investigating the Promising P28 Peptide-Loaded Chitosan/Ceramic Bone Scaffolds for Bone Regeneration}, journal = {Molecules}, year = {2024}, month = {sep}, url = {https://doi.org/10.3390/molecules29174208}, doi = {10.3390/molecules29174208} } . Molecules.

Gustavo Fehrenbach, Keran Zhou, Bianca Simonassi-Paiva, Guangming Yan, Alexandre Portela, Robert Pogue, Zhi Cao, Margaret Brennan Fournet, Declan M. Devine (2024). Investigating the Promising P28 Peptide-Loaded Chitosan/Ceramic Bone Scaffolds for Bone Regeneration . Molecules.

Evaluation of Dunaliella salina Growth in Different Salinities for Potential Application in Saline Water Treatment and Biomass Production @article{6d7e0b794ac64430b776805597124ebd, title = "Evaluation of Dunaliella salina Growth in Different Salinities for Potential Application in Saline Water Treatment and Biomass Production", abstract = "This study investigated the adaptability of Dunaliella salina to different salinity levels, with an emphasis on growth, pigment concentration, and desalination potential. It was found that among the 21 salinity levels, Salinity 75 produced consistently favorable results in cell count (13.08 × 10 3 ± 1.41 × 10 3 cells/mL), dry biomass (2.46 ± 0.06 g/L), pigment content (chlorophyll a = 97,500,000 ± 100,000 pg/L, chlorophyll b = 123,600,000 ± 300,000 pg/L), and desalination (9.32 ± 0.47 reduction). Therefore, Salinity 75 was selected for the final trial (scale-up), which revealed unanticipatedly high cell counts (58.96 × 10 3 ± 535.22 cells/mL), with the dry biomass weight being statistically different (higher) than expected (4.21 ± 0.02 g/L) (p < 0.0001), most likely due to the high cell count and energy reserve storage for high-salinity adaption in the form of bio-compounds. Pigment growth continued (chlorophyll a = 95,400,000 ± 2,200,000 pg/L, chlorophyll b = 128,100,000 ± 5,100,000 pg/L), indicating pigment production under salt stress. Notably, desalination did not occur in this stage, possibly due to the necessity for a bigger initial inoculate, prolonged exposure or bioaccumulation becoming the prevailing mechanism over desalination. Nevertheless, the trial highlights D. salina{\textquoteright}s strong adaptation to various salinity levels. This suggests a promising future in halophyte research, particularly in understanding the mechanisms that prevent salt accumulation in cells and how to overcome this barrier. Additionally, these results suggest that microalgae could be a viable resource in saline-rich environments unsuitable for conventional agriculture, promoting industrial adaptation to adverse conditions.", keywords = "Dunaliella salina, cell count, desalination, dry biomass, pigment, upscaling", author = "J.R. Tanoeiro and G.W. Fehrenbach and P. Murray and R. Pedrosa and Y. Chen", note = "Publisher Copyright: {\textcopyright} 2024 by the authors.", year = "2024", month = sep, doi = "10.3390/aquacj4030007", language = "English", volume = "4", pages = "92--103", journal = "Aquaculture Journal", issn = "2673-9496", publisher = "Multidisciplinary Digital Publishing Institute (MDPI)", number = "3", } . Aquaculture Journal.

Investigating the Promising P28 Peptide-Loaded Chitosan/Ceramic Bone Scaffolds for Bone Regeneration @article{4e9ed12a96d047c3a2dc74db2fe420cd, title = "Investigating the Promising P28 Peptide-Loaded Chitosan/Ceramic Bone Scaffolds for Bone Regeneration", abstract = "Bone has the ability to heal itself; however, bone defects fail to heal once the damage exceeds a critical size. Bone regeneration remains a significant clinical challenge, with autograft considered the ideal bone graft material due to its sufficient porosity, osteogenic cells, and biological growth factors. However, limitations to bone grafting, such as limited bone stock and high resorption rates, have led to a great deal of research into developing bone graft substitutes. The P28 peptide is a small molecule bioactive biomimetic alternative to mimic the bone morphogenetic protein 2 (BMP-2). In this study, we investigated the potential of P28-loaded hybrid scaffolds to mimic the natural bone structure for enhancing the bone regeneration process. We hypothesized that the peptide-loaded scaffolds and nude scaffolds both have the potential to promote bone healing, and the bone healing process is accelerated by the release of the peptide. To verify our hypothesis, C2C12 cells were evaluated for the presence of calcium deposits by histological stain at 7 and 14 days in cultures with hybrid scaffolds. Total RNA was isolated from C2C12 cells cultured with hybrid scaffolds for 7 and 14 days to assess osteoblast differentiation. The project findings demonstrated that the hybrid scaffold could enhance osteoblast differentiation and significantly improve the therapeutic effects of the scaffold in bone regeneration.", keywords = "bone regeneration, bone scaffold, bone tissue engineering, osteoblast differentiation", author = "Keran Zhou and \{Simonassi Raso de Paiva\}, Bianca and Guangming Yan and Alexandre Portela and Robert Pogue and Zhi Cao and Devine, \{Declan M.\} and \{Brennan Fournet\}, Margaret and Gustavo Fehrenbach", note = "Publisher Copyright: {\textcopyright} 2024 by the authors.", year = "2024", month = sep, doi = "10.3390/molecules29174208", language = "English", volume = "29", journal = "Molecules", issn = "1420-3049", publisher = "Multidisciplinary Digital Publishing Institute (MDPI)", number = "17", } . Molecules.

Jo&#227;o Rui Tanoeiro, Gustavo W. Fehrenbach, Patrick Murray, Rui Pedrosa, Yuanyuan Chen (2024). Evaluation of Dunaliella salina Growth in Different Salinities for Potential Application in Saline Water Treatment and Biomass Production . Aquaculture Journal.

Ismin Zainol Abidin, Emma J. Murphy, Gustavo W. Fehrenbach, Noel Gately, Ian Major (2024). Chitosan-(poly)acrylic acid polyelectrolyte complexes: Enhanced mucoadhesion and sustained drug release in vaginal tablets . Carbohydrate Polymer Technologies and Applications.

Chitosan-(poly)acrylic acid polyelectrolyte complexes @article{a5bbb9903b74468d84f960ae3ce94e0f, title = "Chitosan-(poly)acrylic acid polyelectrolyte complexes: Enhanced mucoadhesion and sustained drug release in vaginal tablets", abstract = "Polyelectrolyte complexes (PECs) have emerged as promising vehicles for medical applications, attributable to their non-toxicity, non-immunogenicity, and ability for controlled drug release. Notably, their inherent mucoadhesive properties are being leveraged for sustained drug delivery across mucosal barriers, including vaginal, ocular, nasal, and buccal membranes. This study delves into the formulation and effects of chitosan-(poly)acrylic acid-based PEC for vaginal tablet applications. A comparative analysis of various polymer combinations was conducted, emphasizing the differential impacts of low versus high-molecular weight chitosan. Results highlighted that PECs augmented the flowability of powder mixtures and bolstered the tablets' physical properties. Moreover, PEC-enhanced formulations exhibited a pronounced increase in swelling efficiency, with swelling index values reaching up to 92.67 \% ± 1.90, ensuring structural integrity during dissolution. In ex vivo mucoadhesion tests, tablets from PEC mixes demonstrated extended adhesion, surpassing 48 h for certain formulations. The optimized PEC formulation successfully achieved sustained drug release while retaining tablet adhesion for the duration of the release period.", keywords = "(Poly)acrylic acid, Chitosan, Mucoadhesive vaginal tablet, Polyelectrolyte complex, Sustained release, Swelling", author = "Abidin, \{Ismin Zainol\} and Murphy, \{Emma J.\} and Noel Gately and Ian Major and Gustavo Fehrenbach", note = "Publisher Copyright: {\textcopyright} 2024 The Authors", year = "2024", month = jun, doi = "10.1016/j.carpta.2024.100480", language = "English", volume = "7", journal = "Carbohydrate Polymer Technologies and Applications", issn = "2666-8939", publisher = "Elsevier Ltd.", } . Carbohydrate Polymer Technologies and Applications.

Gustavo Waltzer Fehrenbach, Emma Murphy, Robert Pogue, Frank Carter, Eoghan Clifford, Ian Major (2024). Comprehensive analysis and assessment of exposure to enteric viruses and bacteria in shellfish . Marine Environmental Research.

Comprehensive analysis and assessment of exposure to enteric viruses and bacteria in shellfish @article{be6334c8fef54a659a2b97d9508ccda9, title = "Comprehensive analysis and assessment of exposure to enteric viruses and bacteria in shellfish", abstract = "Shellfish species, including oysters, clams, and mussels, are extensively cultured in coastal waters. Its location is determined by factors such as nutrient availability, water temperature, tidal cycle, and the presence of contaminants such as Escherichia coli and enteric viruses. With the expansion and intensification of human activities at vicinities, the presence of anthropogenic contaminants has increased, threatening shellfish farms and consumer safety give the prevalent consumption of raw shellfish. This literature review aims to provide a comprehensive analysis of the dietary exposure and assess the risk associated with enteric viruses and bacteria detected in shellfish. The predominant bacteria and viruses detected in shellfish are reported, and the potential interrelation is discussed. The main characteristics of each contaminant and shellfish were reviewed for a more comprehensive understanding. To facilitate a direct estimation of exposure, the estimated daily intake (EDI) of bacteria was calculated based on the average levels of E. coli in shellfish, as reported in the literature. The mean daily ingestion of seafood in each of the five continents was considered. Asia exhibited the highest intake of contaminants, with an average of ±5.6 E. coli units/day.kg body weight in cockles. Simulations were conducted using recommended shellfish consumption levels established by state agencies, revealing significantly lower (p < 0.01) EDI for all continents compared to estimations based on recommended levels. This indicates a higher risk associated with healthy shellfish ingestion, potentially leading to increased intoxication incidents with a change in dietary habits. To promote a healthier lifestyle through increased shellfish consumptions, it is imperative to reduce the exposure of shellfish species to bacteria and enteric viruses. The conventional use of E. coli as the sole indicator for consumption safety and water quality in shellfish farms has been deemed insufficient. Instances where shellfish met E. coli limits established by state agencies were often found to be contaminated with human enteric viruses. Therefore, a holistic approach considering the entire production chain is necessary to support the shellfish industry and ensure food safety.", keywords = "Bacteria, Exposure assessment, Seafood safety, Seawater contamination, Viruses", author = "Fehrenbach, \{Gustavo Waltzer\} and Emma Murphy and Robert Pogue and Frank Carter and Eoghan Clifford and Ian Major", note = "Publisher Copyright: {\textcopyright} 2024 The Authors", year = "2024", month = apr, doi = "10.1016/j.marenvres.2024.106404", language = "English", volume = "196", journal = "Marine Environmental Research", issn = "0141-1136", publisher = "Elsevier Ltd.", } . Marine Environmental Research.

Chitosan-(Poly)acrylic Acid Polyelectrolyte Complexes: Enhanced Mucoadhesion and Sustained Drug Release in Vaginal Tablets @article { author = {Ismin Zainol Abidin and Emma J. Murphy and Gustavo W. Fehrenbach and Noel Gately and Ian Major}, title = {Chitosan-(Poly)acrylic Acid Polyelectrolyte Complexes: Enhanced Mucoadhesion and Sustained Drug Release in Vaginal Tablets}, journal = {Carbohydrate Polymer Technologies and Applications}, year = {2024}, month = {mar}, url = {http://dx.doi.org/10.1016/j.carpta.2024.100480}, doi = {10.1016/j.carpta.2024.100480} } . Carbohydrate Polymer Technologies and Applications.

Ismin Zainol Abidin, Emma J. Murphy, Gustavo W. Fehrenbach, Noel Gately, Ian Major (2024). Chitosan-(Poly)acrylic Acid Polyelectrolyte Complexes: Enhanced Mucoadhesion and Sustained Drug Release in Vaginal Tablets . Carbohydrate Polymer Technologies and Applications.

Tanoeiro, J.R., Fehrenbach, G.W., Murray, P., Pedrosa, R., Chen, Y.(2024). Evaluation of Dunaliella salina Growth in Different Salinities for Potential Application in Saline Water Treatment and Biomass Production . Aquaculture Journal. 4. (3). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 92-103.

Fehrenbach, G.W., Murphy, E., Pogue, R., Carter, F., Clifford, E., Major, I.(2024). Comprehensive analysis and assessment of exposure to enteric viruses and bacteria in shellfish . Marine Environmental Research. 196.

Abidin, I.Z., Murphy, E.J., Fehrenbach, G.W., Gately, N., Major, I.(2024). Chitosan-(poly)acrylic acid polyelectrolyte complexes: Enhanced mucoadhesion and sustained drug release in vaginal tablets . Carbohydrate Polymer Technologies and Applications. 7.

Zhou, K., Simonassi-Paiva, B., Fehrenbach, G., Yan, G., Portela, A., Pogue, R., Cao, Z., Fournet, M.B., Devine, D.M.(2024). Investigating the Promising P28 Peptide-Loaded Chitosan/Ceramic Bone Scaffolds for Bone Regeneration . Molecules. 29. (17).

Comprehensive analysis and assessment of exposure to enteric viruses and bacteria in shellfish @article { author = {Fehrenbach, G.W. and Murphy, E. and Pogue, R. and Carter, F. and Clifford, E. and Major, I.}, title = {Comprehensive analysis and assessment of exposure to enteric viruses and bacteria in shellfish}, journal = {Marine Environmental Research}, volume = {196}, year = {2024}, url = {https://www.scopus.com/inward/record.url?eid=2-s2.0-85184764059&partnerID=MN8TOARS}, doi = {10.1016/j.marenvres.2024.106404} } . Marine Environmental Research.

de M. de Lima, T.A., de Lima, G.G., Rouxel, P., Bezerra, G.S.N., Fehrenbach, G.W., Magalh&#227;es, W.L.E., Nugent, M.J.D.(2024). Extraction and characterization of microfibrillated cellulose (MFC) from Rhododendron ponticum isolated using cryocrush pre-treatment and its potential for mycelium cultivation . International Journal of Biological Macromolecules. 279.

Evaluation of Dunaliella salina Growth in Different Salinities for Potential Application in Saline Water Treatment and Biomass Production @article { author = {Tanoeiro, J.R. and Fehrenbach, G.W. and Murray, P. and Pedrosa, R. and Chen, Y.}, title = {Evaluation of Dunaliella salina Growth in Different Salinities for Potential Application in Saline Water Treatment and Biomass Production}, journal = {Aquaculture Journal}, volume = {4}, number = {3}, pages = {92--103}, year = {2024}, url = {https://www.scopus.com/inward/record.url?eid=2-s2.0-105010276051&partnerID=MN8TOARS}, doi = {10.3390/aquacj4030007} } . Aquaculture Journal.

Extraction and characterization of microfibrillated cellulose (MFC) from Rhododendron ponticum isolated using cryocrush pre-treatment and its potential for mycelium cultivation @article { author = {de M. de Lima, T.A. and de Lima, G.G. and Rouxel, P. and Bezerra, G.S.N. and Fehrenbach, G.W. and Magalhães, W.L.E. and Nugent, M.J.D.}, title = {Extraction and characterization of microfibrillated cellulose (MFC) from Rhododendron ponticum isolated using cryocrush pre-treatment and its potential for mycelium cultivation}, journal = {International Journal of Biological Macromolecules}, volume = {279}, year = {2024}, url = {https://www.scopus.com/inward/record.url?eid=2-s2.0-85203285175&partnerID=MN8TOARS}, doi = {10.1016/j.ijbiomac.2024.135284} } . International Journal of Biological Macromolecules.

The Influence of the Molecular Weight of Poly(Ethylene Oxide) on the Hydrolytic Degradation and Physical Properties of Polycaprolactone Binary Blends @article{bc9ab39579a2472db0e75fe0e32044dd, title = "The Influence of the Molecular Weight of Poly(Ethylene Oxide) on the Hydrolytic Degradation and Physical Properties of Polycaprolactone Binary Blends", abstract = "The use of biodegradable polymers in tissue engineering has been widely researched due to their ability to degrade and release their components in a controlled manner, allowing for the potential regeneration of tissues. Melt blending is a common method for controlling the degradation rate of these polymers, which involves combining these materials in a molten state to create a homogenous mixture with tailored properties. In this study, polycaprolactone (PCL) was melt blended with hydrophilic poly (ethylene oxide) (PEO) of different molecular weights to assess its effect on PCL material performance. Hydrolytic degradation, thermal and viscoelastic properties, and surface hydrophilicity were performed to contrast the properties of the blends. DSC, DMA, and FTIR were performed on selected degraded PCL/PEO specimens following mass loss studies. The results showed that adding PEO to PCL reduced its melt viscosity-torque and melt temperature while increasing its hydrophilicity, optimizing PCL/PEO blend for soft tissue engineering applications and could contribute to the development of more effective and biocompatible materials for soft tissue regeneration.", keywords = "biodegradation, hot melt extrusion, melt blending, poly(ethylene oxide), polycaprolactone", author = "Maurice Dalton and Farnoosh Ebrahimi and Han Xu and Ke Gong and Evert Fuenmayor and Murphy, \{Emma J.\} and Ian Major and Gustavo Fehrenbach", note = "Publisher Copyright: {\textcopyright} 2023 by the authors.", year = "2023", month = sep, doi = "10.3390/macromol3030026", language = "English", volume = "3", pages = "431--450", journal = "Macromol", issn = "2673-6209", publisher = "Multidisciplinary Digital Publishing Institute (MDPI)", number = "3", } . Macromol.

The Influence of the Molecular Weight of Poly(Ethylene Oxide) on the Hydrolytic Degradation and Physical Properties of Polycaprolactone Binary Blends @article { author = {Maurice Dalton and Farnoosh Ebrahimi and Han Xu and Ke Gong and Gustavo Fehrenbach and Evert Fuenmayor and Emma J. Murphy and Ian Major}, title = {The Influence of the Molecular Weight of Poly(Ethylene Oxide) on the Hydrolytic Degradation and Physical Properties of Polycaprolactone Binary Blends}, journal = {Macromol}, volume = {3}, number = {3}, pages = {431--450}, year = {2023}, month = {jul}, url = {http://dx.doi.org/10.3390/macromol3030026}, doi = {10.3390/macromol3030026} } . Macromol.

Gustavo Fehrenbach, Maurice Dalton, Farnoosh Ebrahimi, Han Xu, Ke Gong, Evert Fuenmayor, Emma J. Murphy, Ian Major(2023). The Influence of the Molecular Weight of Poly(Ethylene Oxide) on the Hydrolytic Degradation and Physical Properties of Polycaprolactone Binary Blends . Macromol. 3. (3). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 431--450. {MDPI} {AG}

Pulsed ultraviolet (PUV) disinfection of artificially contaminated seawater seeded with high levels of pathogen disease indicators as an alternative for the shellfish industry depuration systems @article{40f8328671944a01ba260f78ff37d600, title = "Pulsed ultraviolet (PUV) disinfection of artificially contaminated seawater seeded with high levels of pathogen disease indicators as an alternative for the shellfish industry depuration systems", abstract = "The increase in pathogen levels in seawater threatens the safety of entire aquatic ecosystems. Foodborne pathogens can potentially accumulate in shellfish, especially in filter feeders such as bivalves, requiring an efficient depuration process before consumption. Alternative approaches to promote a cost-efficient purge at depuration plants are urgently needed. A small prototype pulsed ultraviolet (PUV) light recirculation system was designed, and its depuration potential was tested in a seawater matrix artificially contaminated with high levels of microbial pathogens Escherichia coli, Staphylococcus aureus, Salmonella typhimurium, Bacillus cereus and Candida albicans. The analysis of treatment parameters including voltage, number of pulses and duration of treatment was performed to ensure the highest reduction in contaminant levels. Optimal PUV disinfection was attained at 60 pulses/min at 1 kV for 10 min (a UV output of 12.9 J/cm2). All reductions were statistically significant, and the greatest was observed for S. aureus (5.63 log10), followed by C. albicans (5.15 log10), S. typhimurium (5 log10), B. cereus (4.59 log10) and E. coli (4.55 log10). PUV treatment disrupted the pathogen DNA with the result that S. aureus, C. albicans and S. typhimurium were not detectable by PCR. Regulations were reviewed to address the applicability of PUV treatment as a promising alternative to assist in the reduction of microbial pathogens at depuration plants due to its high efficiency, short treatment period, high UV dose and recirculation system as currently employed in shellfish depuration plants.", keywords = "Depuration, Disinfection, Foodborne pathogens, Seafood, Ultraviolet", author = "Gustavo Fehrenbach and Emma Murphy and Robert Pogue and Frank Carter and Eoghan Clifford and Ian Major and Neil Rowan", note = "Publisher Copyright: {\textcopyright} 2023, The Author(s).", year = "2023", month = jun, doi = "10.1007/s11356-023-27286-6", language = "English", volume = "30", pages = "70771--70782", journal = "Environmental Science and Pollution Research", issn = "0944-1344", publisher = "Springer", number = "27", } . Environmental Science and Pollution Research.

Emma J. Murphy, Gustavo Waltzer Fehrenbach, Ismin Zainol Abidin, Ciara Buckley, Therese Montgomery, Robert Pogue, Patrick Murray, Ian Major, Emanuele Rezoagli (2023). Polysaccharides—Naturally Occurring Immune Modulators . Polymers.

Gustavo Waltzer Fehrenbach, Emma Murphy, Robert Pogue, Frank Carter, Eoghan Clifford, Ian Major, Neil Rowan (2023). Pulsed ultraviolet (PUV) disinfection of artificially contaminated seawater seeded with high levels of pathogen disease indicators as an alternative for the shellfish industry depuration systems . Environmental Science and Pollution Research.

Emma J. Murphy, Gustavo Waltzer Fehrenbach, Ismin Zainol Abidin, Ciara Buckley, Therese Montgomery, Robert Pogue, Patrick Murray, Ian Major, Emanuele Rezoagli (2023). Polysaccharides—Naturally Occurring Immune Modulators . Polymers.

Polysaccharides—Naturally Occurring Immune Modulators @article{e275ce028e3443abb1fee1af85fd1a85, title = "Polysaccharides—Naturally Occurring Immune Modulators", abstract = "The prevention of disease and infection requires immune systems that operate effectively. This is accomplished by the elimination of infections and abnormal cells. Immune or biological therapy treats disease by either stimulating or inhibiting the immune system, dependent upon the circumstances. In plants, animals, and microbes, polysaccharides are abundant biomacromolecules. Due to the intricacy of their structure, polysaccharides may interact with and impact the immune response; hence, they play a crucial role in the treatment of several human illnesses. There is an urgent need for the identification of natural biomolecules that may prevent infection and treat chronic disease. This article addresses some of the naturally occurring polysaccharides of known therapeutic potential that have already been identified. This article also discusses extraction methods and immunological modulatory capabilities.", keywords = "immune modulation, inflammation, naturally occurring polymers, polysaccharides", author = "Murphy, \{Emma J.\} and Fehrenbach, \{Gustavo Waltzer\} and Abidin, \{Ismin Zainol\} and Ciara Buckley and Therese Montgomery and Robert Pogue and Patrick Murray and Ian Major and Emanuele Rezoagli", note = "Publisher Copyright: {\textcopyright} 2023 by the authors.", year = "2023", month = may, doi = "10.3390/polym15102373", language = "English", volume = "15", journal = "Polymers", issn = "2073-4360", publisher = "Multidisciplinary Digital Publishing Institute (MDPI)", number = "10", } . Polymers.

Gustavo Waltzer Fehrenbach (2023). Bioremediation and biomass production by Chlorella sp. isolated from effluent treatment station of a parboiled rice industry . Natural Resources.

Ismin Zainol Abidin, Emma Murphy, Gustavo Waltzer Fehrenbach, Emanuele Rezoagli, Noel Gately, Ian Major (2023). A systematic review of mucoadhesive vaginal tablet testing . Drug Target Insights.

Murphy, E.J., Fehrenbach, G.W., Abidin, I.Z., Buckley, C., Montgomery, T., Pogue, R., Murray, P., Major, I., Rezoagli, E.(2023). Polysaccharides—Naturally Occurring Immune Modulators . Polymers. 15. (10).

A systematic review of mucoadhesive vaginal tablet testing @article { author = {Abidin, I.Z. and Murphy, E.J. and Fehrenbach, G.W. and Rezoagli, E. and Gately, N. and Major, I.}, title = {A systematic review of mucoadhesive vaginal tablet testing}, journal = {Drug Target Insights}, volume = {17}, pages = {5--30}, year = {2023}, url = {https://www.scopus.com/inward/record.url?eid=2-s2.0-85146804043&partnerID=MN8TOARS}, doi = {10.33393/dti.2023.2477} } . Drug Target Insights.

Abidin, I.Z., Murphy, E.J., Fehrenbach, G.W., Rezoagli, E., Gately, N., Major, I.(2023). A systematic review of mucoadhesive vaginal tablet testing . Drug Target Insights. 17. Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 5-30.

Ismin Zainol Abidin, Emma J. Murphy, Gustavo Waltzer Fehrenbach, Emanuele Rezoagli, Noel Gately, Ian Major(2023). A systematic review of mucoadhesive vaginal tablet testing . Drug Target Insights. 17. Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 5--30. AboutScience Srl

Dalton, M., Ebrahimi, F., Xu, H., Gong, K., Fehrenbach, G., Fuenmayor, E., Murphy, E.J., Major, I.(2023). The Influence of the Molecular Weight of Poly(Ethylene Oxide) on the Hydrolytic Degradation and Physical Properties of Polycaprolactone Binary Blends . Macromol. 3. (3). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 431-450.

Fehrenbach, G.W., Murphy, E., Pogue, R., Carter, F., Clifford, E., Major, I., Rowan, N.(2023). Pulsed ultraviolet (PUV) disinfection of artificially contaminated seawater seeded with high levels of pathogen disease indicators as an alternative for the shellfish industry depuration systems . Environmental Science and Pollution Research. 30. (27). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 70771-70782.

Polysaccharides—Naturally Occurring Immune Modulators @article { author = {Murphy, E.J. and Fehrenbach, G.W. and Abidin, I.Z. and Buckley, C. and Montgomery, T. and Pogue, R. and Murray, P. and Major, I. and Rezoagli, E.}, title = {Polysaccharides—Naturally Occurring Immune Modulators}, journal = {Polymers}, volume = {15}, number = {10}, year = {2023}, url = {https://www.scopus.com/inward/record.url?eid=2-s2.0-85160600047&partnerID=MN8TOARS}, doi = {10.3390/polym15102373} } . Polymers.

Pulsed ultraviolet (PUV) disinfection of artificially contaminated seawater seeded with high levels of pathogen disease indicators as an alternative for the shellfish industry depuration systems @article { author = {Fehrenbach, G.W. and Murphy, E. and Pogue, R. and Carter, F. and Clifford, E. and Major, I. and Rowan, N.}, title = {Pulsed ultraviolet (PUV) disinfection of artificially contaminated seawater seeded with high levels of pathogen disease indicators as an alternative for the shellfish industry depuration systems}, journal = {Environmental Science and Pollution Research}, volume = {30}, number = {27}, pages = {70771--70782}, year = {2023}, url = {https://www.scopus.com/inward/record.url?eid=2-s2.0-85158142650&partnerID=MN8TOARS}, doi = {10.1007/s11356-023-27286-6} } . Environmental Science and Pollution Research.

Use of next generation sequencing and bioinformatics for profiling freshwater eukaryotic microalgae in a novel peatland integrated multi-trophic aquaculture (IMTA) system @article{9a42992a09ad454288c6ad9c25fc6d30, title = "Use of next generation sequencing and bioinformatics for profiling freshwater eukaryotic microalgae in a novel peatland integrated multi-trophic aquaculture (IMTA) system: Case study from the Republic of Ireland", abstract = "Development of integrated multi-trophic aquaculture (IMTA) systems constitutes a step change in the sustainable production of freshwater fish to meet emerging needs for high-protein foods globally. Recently, there has been a paradigm shift away from harvesting peat as a fuel towards the development of wettable peatland innovation (termed {\textquoteleft}paludiculture{\textquoteright}), such as aquaculture. Such eco-innovations support carbon sequestration and align with a balanced environmental approach to protecting biodiversity. This novel peatland-based IMTA process in the Irish midlands relies upon natural microalgae for waste treatment, recirculation and water quality where there is no use of pesticides or antibiotics. This novel IMTA system is powered with a wind turbine and the process has {\textquoteleft}organic status{\textquoteright}; moreover, it does not discharge aquaculture effluent to receiving water. However, there is a significant lack of understanding as to diversity of microalgae in this {\textquoteleft}paludiculture{\textquoteright}-based IMTA processes. This constitutes the first case study to use conventional microscopy combined with next-generation sequencing and bioinformatics to profile microalgae occurring in this novel IMTA system from pooled samples over a 12 month period in 2020. Conventional microscopy combined with classic identification revealed twenty genera of algae; with Chlorophyta and Charophyta being the most common present. However, algal DNA isolation, 16 s sequencing and bioinformatics revealed a combined total of 982 species from 341 genera across nine phyla from the same IMTA system, which emphasized a significant underestimation in the number and diversity of beneficial or potentially harmful algae in the IMTA-microbiome. These new methods also yield rich data that can be used by digital technologies to transform future monitoring and performance of the IMTA system for sustainability. The findings of this study align with many sustainability development goals of the United Nations including no poverty, zero hunger, good health and well-being, responsible consumption and production, climate change, and life below water.", keywords = "Algae, Aquaculture, Ecosystem, Food security, Next-generation sequencing, Sustainability", author = "O'Neill, \{Emer A.\} and Gustavo Fehrenbach and Emma Murphy and Alencar, \{S{\'e}rgio A.\} and Robert Pogue and Rowan, \{Neil J.\}", note = "Publisher Copyright: {\textcopyright} 2022 The Authors", year = "2022", month = dec, day = "10", doi = "10.1016/j.scitotenv.2022.158392", language = "English", volume = "851", journal = "Science of the Total Environment", issn = "0048-9697", publisher = "Elsevier B.V.", } . Science of the Total Environment.

Use of next generation sequencing and bioinformatics for profiling freshwater eukaryotic microalgae in a novel peatland integrated multi-trophic aquaculture (IMTA) system: Case study from the Republic of Ireland @article { author = {Emer A. O'Neill and Gustavo Fehrenbach and Emma Murphy and Sérgio A. Alencar and Robert Pogue and Neil J. Rowan}, title = {Use of next generation sequencing and bioinformatics for profiling freshwater eukaryotic microalgae in a novel peatland integrated multi-trophic aquaculture (IMTA) system: Case study from the Republic of Ireland}, journal = {Science of The Total Environment}, volume = {851}, pages = {158392--158392}, year = {2022}, month = {dec}, url = {http://dx.doi.org/10.1016/j.scitotenv.2022.158392}, doi = {10.1016/j.scitotenv.2022.158392} } . Science of The Total Environment.

Gustavo Fehrenbach, Emer A. O&#39;Neill, Emma Murphy, S&#233;rgio A. Alencar, Robert Pogue, Neil J. Rowan(2022). Use of next generation sequencing and bioinformatics for profiling freshwater eukaryotic microalgae in a novel peatland integrated multi-trophic aquaculture (IMTA) system: Case study from the Republic of Ireland . Science of The Total Environment. 851. Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 158392. Elsevier {BV}

Implications for the seafood industry, consumers and the environment arising from contamination of shellfish with pharmaceuticals, plastics and potentially toxic elements @article{3aa64503fb2b463bb775b703b8fcab7b, title = "Implications for the seafood industry, consumers and the environment arising from contamination of shellfish with pharmaceuticals, plastics and potentially toxic elements: A case study from Irish waters with a global orientation", abstract = "Shellfish are a rich source of minerals, B-vitamins and omega-3 to the human diet. The global population is expected to reach 9.6 billion people by 2050 where there will be increased demand for shellfish and for sustained improvements in harvesting. The production of most consumed species of shellfish is sea-based and are thus susceptible to in situ environmental conditions and water quality. Population growth has contributed to expansion of urbanization and the generation of effluent and waste that reaches aquatic environments, potentially contaminating seafood by exposure to non-treated effluents or inappropriately discarded waste. Environmental contaminants as microplastics (MP), pharmaceuticals (PHAR) and potentially toxic contaminants (PTE) are being identified in all trophic levels and are a current threat to both shellfish and consumer safety. Immunotoxicity, genotoxicity, fertility reduction, mortality and bioaccumulation of PTE are representative examples of the variety of effects already established in contaminated shellfish. In humans, the consumption of contaminated shellfish can lead to neurological and developmental effects, reproductive and gastrointestinal disorders and in extreme cases, death. This timely review provides insights into the presence of MP, PHAR and PTE in shellfish, and estimate the daily intake and hazard quotient for consumption behaviours. Alternatives approaches for seafood depuration that encompass risk reduction are addressed, to reflect state of the art knowledge from a Republic of Ireland perspective. Review of best-published literature revealed that MP, PHAR and PTE contaminants were detected in commercialised species of shellfish, such as Crassostrea and Mytilus. The ability to accumulate these contaminants by shellfish due to feeding characteristics is attested by extensive in vitro studies. However, there is lack of knowledge surrounding the distribution of these contaminants in the aquatic environment their interactions with humans. Preventive approaches including risk assessment are necessary to safeguard the shellfish industry and the consumer.", keywords = "Contamination, Pharmaceuticals, Plastics, Potentially toxic elements, Risk mitigation, Shellfish", author = "Gustavo Fehrenbach and Robert Pogue and Frank Carter and Eoghan Clifford and Neil Rowan", note = "Publisher Copyright: {\textcopyright} 2022 The Authors", year = "2022", month = oct, day = "20", doi = "10.1016/j.scitotenv.2022.157067", language = "English", volume = "844", journal = "Science of the Total Environment", issn = "0048-9697", publisher = "Elsevier B.V.", } . Science of the Total Environment.

Gustavo Waltzer Fehrenbach and Robert Pogue and Frank Carter and Eoghan Clifford and Neil Rowan(2022). Implications for the seafood industry, consumers and the environment arising from contamination of shellfish with pharmaceuticals, plastics and potentially toxic elements: A case study from Irish waters with a global orientation . Science of The Total Environment. Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 157067. Elsevier {BV}

Implications for the seafood industry, consumers and the environment arising from contamination of shellfish with pharmaceuticals, plastics and potentially toxic elements: A case study from Irish waters with a global orientation @article { title = {Implications for the seafood industry, consumers and the environment arising from contamination of shellfish with pharmaceuticals, plastics and potentially toxic elements: A case study from Irish waters with a global orientation}, journal = {Science of The Total Environment}, pages = {157067--157067}, year = {2022}, month = {jun}, url = {http://dx.doi.org/10.1016/j.scitotenv.2022.157067}, doi = {10.1016/j.scitotenv.2022.157067} } . Science of The Total Environment.

Gustavo Fehrenbach, Aline Sitowski, Diogo Novo, M&#225;rcia Mesko, Diego Santos, F&#225;bio Leite (2022). Nutrient Removal and Biomass Production by Culturing Saccharomyces Cerevisiae in Parboiled Rice Effluent . Ecological Engineering & Environmental Technology.

Immunomodulatory activity of β-glucan polysaccharides isolated from different species of mushroom – A potential treatment for inflammatory lung conditions @article{94f156815ed7494bbc0bf537b9bf1d11, title = "Immunomodulatory activity of β-glucan polysaccharides isolated from different species of mushroom – A potential treatment for inflammatory lung conditions", abstract = "Acute respiratory distress syndrome (ARDS) is the most common form of acute severe hypoxemic respiratory failure in the critically ill with a hospital mortality of 40\%. Alveolar inflammation is one of the hallmarks for this disease. β-Glucans are polysaccharides isolated from a variety of natural sources including mushrooms, with documented immune modulating properties. To investigate the immunomodulatory activity of β-glucans and their potential as a treatment for ARDS, we isolated and measured glucan-rich polysaccharides from seven species of mushrooms. We used three models of in-vitro injury in THP-1 macrophages, Peripheral blood mononuclear cells (CD14+) (PMBCs) isolated from healthy volunteers and lung epithelial cell lines. We observed variance between β-glucan content in extracts isolated from seven mushroom species. The extracts with the highest β-glucan content found was Lentinus edodes which contained 70\% w/w and Hypsizygus tessellatus which contained 80\% w/w with low levels of α-glucan. The extracts had the ability to induce secretion of up to 4000 pg/mL of the inflammatory cytokine IL-6, and up to 5000 pg/mL and 500 pg/mL of the anti-inflammatory cytokines IL-22 and IL-10, respectively, at a concentration of 1 mg/mL in THP-1 macrophages. In the presence of cytokine injury, IL-8 was reduced from 15,000 pg/mL to as low as 10,000 pg/mL in THP-1 macrophages. After insult with LPS, phagocytosis dropped from 70–90\% to as low 10\% in CD14+ PBMCs. After LPS insult CCL8 relative gene expression was reduced, and IL-10 relative gene expression increased from 50 to 250-fold in THP-1 macrophages. In lung epithelial cells, both A549 and BEAS-2B after IL-1β insult, IL-8 levels dropped from 10,000 pg/mL to as low as 6000 pg/mL. TNF-α levels dropped 10-fold from 100 pg/mL to just below 10 pg/mL. These results demonstrate the therapeutic potential of β-glucans in inflammatory lung conditions. Findings also advance bio-based research that connects green innovation with One Health applications for the betterment of society.", keywords = "ARDS, Lung injury, Medicinal mushrooms, One-health, THP-1 macrophages, β-Glucans", author = "Murphy, \{Emma J.\} and Emanuele Rezoagli and Robert Pogue and Bianca Simonassi-Paiva and Abidin, \{Ismin Izwani Zainol\} and Gustavo Fehrenbach and Emer O'Neil and Ian Major and Laffey, \{John G.\} and Neil Rowan", note = "Publisher Copyright: {\textcopyright} 2021 Elsevier B.V.", year = "2022", month = feb, day = "25", doi = "10.1016/j.scitotenv.2021.152177", language = "English", volume = "809", journal = "Science of the Total Environment", issn = "0048-9697", publisher = "Elsevier B.V.", } . Science of the Total Environment.

Immunomodulatory activity of ß-glucan polysaccharides isolated from different species of mushroom – A potential treatment for inflammatory lung conditions @article { title = {Immunomodulatory activity of ß-glucan polysaccharides isolated from different species of mushroom – A potential treatment for inflammatory lung conditions}, journal = {Science of The Total Environment}, volume = {809}, pages = {152177--152177}, year = {2022}, month = {feb}, url = {http://dx.doi.org/10.1016/j.scitotenv.2021.152177}, doi = {10.1016/j.scitotenv.2021.152177} } . Science of The Total Environment.

Emma J. Murphy and Emanuele Rezoagli and Robert Pogue and Bianca Simonassi-Paiva and Ismin Izwani Zainol Abidin and Gustavo Waltzer Fehrenbach and Emer O{\textquotesingle}Neil and Ian Major and John G. Laffey and Neil Rowan(2022). Immunomodulatory activity of β-glucan polysaccharides isolated from different species of mushroom – A potential treatment for inflammatory lung conditions . Science of The Total Environment. 809. Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 152177. Elsevier {BV}

Murphy, E.J., Rezoagli, E., Pogue, R., Simonassi-Paiva, B., Abidin, I.I.Z., Fehrenbach, G.W., O&#39;Neil, E., Major, I., Laffey, J.G., Rowan, N.(2022). Immunomodulatory activity of β-glucan polysaccharides isolated from different species of mushroom – A potential treatment for inflammatory lung conditions . Science of the Total Environment. 809.

Fehrenbach, G.W., Pogue, R., Carter, F., Clifford, E., Rowan, N.(2022). Implications for the seafood industry, consumers and the environment arising from contamination of shellfish with pharmaceuticals, plastics and potentially toxic elements: A case study from Irish waters with a global orientation . Science of the Total Environment. 844.

O&#39;Neill, E.A., Fehrenbach, G., Murphy, E., Alencar, S.A., Pogue, R., Rowan, N.J.(2022). Use of next generation sequencing and bioinformatics for profiling freshwater eukaryotic microalgae in a novel peatland integrated multi-trophic aquaculture (IMTA) system: Case study from the Republic of Ireland . Science of the Total Environment. 851.

Nutrient Removal and Biomass Production by Culturing Saccharomyces Cerevisiae in Parboiled Rice Effluent @article{671b6a8c593b44a3828acf06f87dc3cf, title = "Nutrient Removal and Biomass Production by Culturing Saccharomyces Cerevisiae in Parboiled Rice Effluent", abstract = "The objective of this study was to evaluate the potential of Saccharomyces cerevisiae in the treatment of rice parboiling effluent (PE) and biomass production. PE was inoculated with S. cerevisiae at 1.2×104 CFU mL-1 and cultured in shaker at 28 °C and 180 rpm for 72 h. PE supplied the required nutrients for S. cerevisiae growth, reach-ing a biomass of ± 8.2 g·L−1, cell viability of ± 2×1011 CFU mL−1 and removals of 74\%, 56\% and 17\% for total Kjedahl nitrogen, chemical oxygen demand and phosphorus, respectively. The versatile of S. cerevisiae supported the direct and non-supplemented cultivation in PE, resulting in high removals of nutrient and biomass production and represent an alternative method to reduce the environmental impact of rice industry and an alternative process to obtain marketable yeast biomass.", keywords = "Saccharomyces cerevisiae, biomass, bioremediation, cell viability, parboiled effluent", author = "Fehrenbach, \{Gustavo Waltzer\} and Aline Sitowski and Novo, \{Diogo La Rosa\} and Mesko, \{M{\'a}rcia Foster\} and \{de los Santos\}, \{Diego Gil\} and Leite, \{F{\'a}bio Pereira Leivas\}", note = "Publisher Copyright: {\textcopyright} 2022, Polskie Towarzystwo Inzynierii Ekologicznej (PTIE). All rights reserved.", year = "2022", doi = "10.12912/27197050/147634", language = "English", volume = "23", pages = "177--183", journal = "Ecological Engineering and Environmental Technology", issn = "2719-7050", publisher = "Polskie Towarzystwo Inzynierii Ekologicznej", number = "3", } . Ecological Engineering and Environmental Technology.

Fehrenbach, G.W., Sitowski, A., Novo, D.R., Mesko, M.F., de los Santos, D.G., Leite, F.P.L.(2022). Nutrient Removal and Biomass Production by Culturing Saccharomyces Cerevisiae in Parboiled Rice Effluent . Ecological Engineering and Environmental Technology. 23. (3). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 177-183.

Nutrient Removal and Biomass Production by Culturing Saccharomyces Cerevisiae in Parboiled Rice Effluent @article { author = {Fehrenbach, G.W. and Sitowski, A. and Novo, D.R. and Mesko, M.F. and de los Santos, D.G. and Leite, F.P.L.}, title = {Nutrient Removal and Biomass Production by Culturing Saccharomyces Cerevisiae in Parboiled Rice Effluent}, journal = {Ecological Engineering and Environmental Technology}, volume = {23}, number = {3}, pages = {177--183}, year = {2022}, url = {https://www.scopus.com/inward/record.url?eid=2-s2.0-85164794845&partnerID=MN8TOARS}, doi = {10.12912/27197050/147634} } . Ecological Engineering and Environmental Technology.

Combined treatment with a-amylase and Saccharomyces cerevisiae culture for nutrient removal and biomass production in effluent from rice parboilization @article { title = {Combined treatment with a-amylase and Saccharomyces cerevisiae culture for nutrient removal and biomass production in effluent from rice parboilization}, journal = {Desalination and Water Treatment}, year = {2021}, month = {dec}, url = {https://www.deswater.com/}, doi = {10.5004/dwt.2021.27886} } . Desalination and Water Treatment.

(2021). Combined treatment with α-amylase and Saccharomyces cerevisiae culture for nutrient removal and biomass production in effluent from rice parboilization . Desalination and Water Treatment.

Combined treatment with α-amylase and Saccharomyces cerevisiae culture for nutrient removal and biomass production in effluent from rice parboilizatio @article{c3c8c536dfe449709c55bcec91c55ca2, title = "Combined treatment with α-amylase and Saccharomyces cerevisiae culture for nutrient removal and biomass production in effluent from rice parboilizatio", abstract = "In this study, we report a non-supplemented and biomass productive treatment for parboiled rice effluent (PE) combining a pre-treatment with α-amylase followed by Saccharomyces cerevisiae cul-ture. PE with amylase (PEA) was pre-treated with 5 mg L–1 of α-amylase for 12.5 min at 50°C – a non-enzymatic culture with PE was used as the negative control. Both cultures were inoculated with 10\% v/v of S. cerevisiae at a concentration of 1.2 × 103 CFU mL–1 for 48 h. The highest level of glucose was 1.75 g L–1 and obtained in PEA culture, 40\% higher than in the control PE. CFU and biomass followed the glucose levels and were 73\% and 62.9\% higher than in PE, with a maximum of 3.35 × 108 CFU mL–1 and 2 g L–1, respectively. The highest removals were obtained in PEA for phosphorus (40.68\%), PE for organic matter (70\%), and 100\% of removal for total nitrogen in both cultures. The low cost of the method (0.45\$ per m3), nutrient removals (40.68\% P, 100\% TKN, 62\% COD), cell viability (3.35 × 108 CFU mL–1) and biomass (2 g L–1) suggests that α-amylase improves the bioremediation potential of S. cerevisiae cultured in PE and can provide a widely marketable biomass as by-product.", keywords = "Bioproducts, Bioremediation, Effluent treatment, Enzymatic treatment, Parboiling process", author = "Fehrenbach, \{Gustavo W.\} and Leite, \{F{\'a}bio P.L.\} and Santos, \{Diego G.L.\}", note = "Publisher Copyright: {\textcopyright} 2021 Desalination Publications. All rights reserved.", year = "2021", month = dec, doi = "10.5004/dwt.2021.27886", language = "English", volume = "243", pages = "200--205", journal = "Desalination and Water Treatment", issn = "1944-3994", publisher = "Elsevier B.V.", } . Desalination and Water Treatment.

A fast, low-cost and eco-friendly method for routine determination of Bisphenol-A in landfill leachate employinvortex assisted liquid-liquid extraction @article{c16ed9053f5f4bffbb44e9cb86a8359b, title = "A fast, low-cost and eco-friendly method for routine determination of Bisphenol-A in landfill leachate employinvortex assisted liquid-liquid extraction", abstract = "Landfills are sites designed to receive and final disposal of a broad variety of urban solid wastes (USW). The decomposition and biodegradation processes generate a leachate of high complexity and toxicity, containing persistent and recalcitrant contaminants that are not usually monitored. Bisphenol-A (BPA) is a synthetic compound applied mostly on the production of polycarbonate plastics, epoxy resins, and is an endocrine disruptor. BPA negatively affects biological receptors, resulting in harmful effects to nervous and reproductive system as well metabolic and immune function. The presence of BPA in USW urges the development of feasible analytical methods to support the effluent treatment plants and reduce the risks of contamination. The main goal of this work was to develop an efficient, eco-friendly, fast and simple method for routine analysis of BPA in the leachate from landfill. A vortex assisted liquid-liquid extraction (VALLME) using 1-octanol as solvent was performed. BPA recoveries at spiking levels of 2.5, 6.5 and 12.5 µg L-1 were between 60 to 104\% with relative standard deviation (RSD) lower than 26\%. The linearity of the method was evaluated and the correlation coefficient was (r) 0.9985. The limit of quantification (LOQ) was 2.5 µg L-1 with a pre-concentration factor of 20. The method has advantages such as low consumption of extraction solvent (150 µL), low cost, easy and fast determination.", keywords = "Bisphenol-A, Contaminant, Endocrine disruptors, Sanitary landfill, VALLME", author = "Fehrenbach, \{Gustavo Waltzer\} and Arsand, \{Daniel Ricardo\} and Barbosa, \{Sergiane Caldas\} and Castagno, \{Katia R.L.\} and Filho, \{Pedro Jose Sanches\} and Primel, \{Ednei Gilberto\}", note = "Publisher Copyright: {\textcopyright} 2021, AMEC Publisher. All rights reserved.", year = "2021", month = sep, day = "30", doi = "10.24200/amecj.v4.i03.146", language = "English", volume = "4", pages = "59--67", journal = "Analytical Methods in Environmental Chemistry Journal", issn = "2645-5552", publisher = "AMEC Publisher", number = "3", } . Analytical Methods in Environmental Chemistry Journal.

Gustavo Fehrenbach and Daniel Ricardo Arsand and Sergiane Caldas Barbosa and K{\'{a}}tia Castagno and Pedro Filho and Ednei Primel(2021). A fast, low-cost and eco-friendly method for routine determination of Bisphenol-A in landfill leachate employing vortex assisted liquid-liquid extraction . Analytical Methods in Environmental Chemistry Journal. 4. (03). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 59--67. {SciTech} Solutions

A fast, low-cost and eco-friendly method for routine determination of Bisphenol-A in landfill leachate employing vortex assisted liquid-liquid extraction @article { title = {A fast, low-cost and eco-friendly method for routine determination of Bisphenol-A in landfill leachate employing vortex assisted liquid-liquid extraction}, journal = {Analytical Methods in Environmental Chemistry Journal}, volume = {4}, number = {03}, pages = {59--67}, year = {2021}, month = {sep}, url = {http://dx.doi.org/10.24200/amecj.v4.i03.146}, doi = {10.24200/amecj.v4.i03.146} } . Analytical Methods in Environmental Chemistry Journal.

Robert Pogue, Emma J. Murphy, Gustavo W. Fehrenbach, Emanuele Rezoagli, Neil J. Rowan (2021). Exploiting immunomodulatory properties of β-glucans derived from natural products for improving health and sustainability in aquaculture-farmed organisms: Concise review of existing knowledge, innovation and future opportunities . Current Opinion in Environmental Science & Health.

Exploiting immunomodulatory properties of β-glucans derived from natural products for improving health and sustainability in aquaculture-farmed organisms @article{65d7c61553f644cab0ad90ca10523b46, title = "Exploiting immunomodulatory properties of β-glucans derived from natural products for improving health and sustainability in aquaculture-farmed organisms: Concise review of existing knowledge, innovation and future opportunities", abstract = "The global aquaculture industry has surpassed capture fishing to achieve goals of economic and environmental sustainability. This intensification, albeit extremely beneficial, has limitations with regard to the cultured animals, including infections, pollution and stress. This can lead to an increase in disease and mortality rates not experienced in natural habitats. Several drugs and vaccination programs have been proposed to address these issues, with only partial success, and as such, there is an urgent requirement for an alternative approach to these threats. One such potential solution is the administration of β-glucans, natural polysaccharides which can induce nonspecific innate defences, ultimately increasing disease resistance. This review will discuss the benefits of the administration of β-glucans in aquaculture by highlighting key data including enhanced immune responses, anti-inflammatory effects, resistance to infection, stress resistance, physical growth improvement, as well as extension of lifespan. We also highlight the important variables to be considered in incorporating β-glucans in aquaculture.", keywords = "Aquaculture, Immunomodulation, Inflammation, Reactive oxygen species, Stress, β-glucans", author = "Robert Pogue and Murphy, \{Emma J.\} and Gustavo Fehrenbach and Emanuele Rezoagli and Rowan, \{Neil J.\}", note = "Publisher Copyright: {\textcopyright} 2021 Elsevier B.V.", year = "2021", month = jun, doi = "10.1016/j.coesh.2021.100248", language = "English", volume = "21", journal = "Current Opinion in Environmental Science and Health", issn = "2468-5844", publisher = "Elsevier B.V.", } . Current Opinion in Environmental Science and Health.

Exploiting immunomodulatory properties of ß-glucans derived from natural products for improving health and sustainability in aquaculture-farmed organisms: Concise review of existing knowledge, innovation and future opportunities @article { author = {Pogue, R. and Murphy, E.J. and Fehrenbach, G.W. and Rezoagli, E. and Rowan, N.J.}, title = {Exploiting immunomodulatory properties of ß-glucans derived from natural products for improving health and sustainability in aquaculture-farmed organisms: Concise review of existing knowledge, innovation and future opportunities}, journal = {Current Opinion in Environmental Science and Health}, volume = {21}, year = {2021}, url = {https://www.scopus.com/inward/record.url?eid=2-s2.0-85104076654&partnerID=MN8TOARS}, doi = {10.1016/j.coesh.2021.100248} } . Current Opinion in Environmental Science and Health.

Pogue, R., Murphy, E.J., Fehrenbach, G.W., Rezoagli, E., Rowan, N.J.(2021). Exploiting immunomodulatory properties of β-glucans derived from natural products for improving health and sustainability in aquaculture-farmed organisms: Concise review of existing knowledge, innovation and future opportunities . Current Opinion in Environmental Science and Health. 21.

Fehrenbach, G.W., Arsand, D.R., Barbosa, S.C., Castagno, K.R.L., Filho, P.J.S., Primel, E.G.(2021). A fast, low-cost and eco-friendly method for routine determination of Bisphenol-A in landfill leachate employinvortex assisted liquid-liquid extraction . Analytical Methods in Environmental Chemistry Journal. 4. (3). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 59-67.

Fehrenbach, G.W., Leite, F.P.L., Santos, D.G.L.(2021). Combined treatment with α-amylase and Saccharomyces cerevisiae culture for nutrient removal and biomass production in effluent from rice parboilizatio . Desalination and Water Treatment. 243. Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 200-205.

A bilayer vaginal tablet for the localized delivery of disulfiram and 5-fluorouracil to the cervix @article{0a073c660d684df4ae003a719213d173, title = "A bilayer vaginal tablet for the localized delivery of disulfiram and 5-fluorouracil to the cervix", abstract = "This study was performed to develop an adjuvant therapy in the form of a self-administered vaginal tablet regimen for the localized delivery of chemotherapeutic drugs. This therapy will help to reduce relapse by eradicating cancerous cells in the margin of cervical tumors. The vaginal tablet is a very common formulation that is easy to manufacture, easy to place in the vagina, and has a low cost of manufacture, making them ideal for use in developing countries. A combination of disulfiram and 5-fluorouracil, which are both off-patent drugs and provide different modes of action, were evaluated. The tablets developed were evaluated for weight variation, thickness, hardness, friability, swelling index, differential scanning calorimetry (DSC), particle morphology, in vitro drug release, and cytotoxicity on Ca-Ski cells. Both layers were designed to release both drugs concurrently for a synergistic effect. The polymer–polymer interaction between the layers was able to reduce the loss of formulation due to chitosan. While the bilayer tablet had satisfactory performance in the physicochemical tests, in vitro cell culture with Ca-Ski also showed a synergistic effect using a combination of drugs at a low dose. However, the formulation only had 24-h dose release before degradation. Further drug combinations should be evaluated in subsequent studies.", keywords = "Bilayer tablets, Cervical cancer, Chitosan, Combination therapy, Mucoadhesion, Polyacrylic acid, Polyelectrolyte complex, Vaginal drug delivery", author = "Abidin, \{Ismin Zainol\} and Emanuele Rezoagli and Bianca Simonassi-Paiva and Kevin Masterson and Robert Pogue and Gustavo Fehrenbach and Zhi Cao and Neil Rowan and Murphy, \{Emma J.\} and Ian Major", note = "Publisher Copyright: {\textcopyright} 2020 by the authors. Licensee MDPI, Basel, Switzerland.", year = "2020", month = dec, doi = "10.3390/pharmaceutics12121185", language = "English", volume = "12", pages = "1--21", journal = "Pharmaceutics", issn = "1999-4923", publisher = "Multidisciplinary Digital Publishing Institute (MDPI)", number = "12", } . Pharmaceutics.

A Bilayer Vaginal Tablet for the Localized Delivery of Disulfiram and 5-Fluorouracil to the Cervix @article { title = {A Bilayer Vaginal Tablet for the Localized Delivery of Disulfiram and 5-Fluorouracil to the Cervix}, journal = {Pharmaceutics}, year = {2020}, url = {https://www.mdpi.com/1999-4923/12/12/1185}, doi = {10.3390/pharmaceutics12121185} } . Pharmaceutics.

(2020). A Bilayer Vaginal Tablet for the Localized Delivery of Disulfiram and 5-Fluorouracil to the Cervix . Pharmaceutics.

Abidin, I.Z., Rezoagli, E., Simonassi-Paiva, B., Fehrenbach, G.W., Masterson, K., Pogue, R., Cao, Z., Rowan, N., Murphy, E.J., Major, I.(2020). A bilayer vaginal tablet for the localized delivery of disulfiram and 5-fluorouracil to the cervix . Pharmaceutics. 12. (12). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 1-21.

CONFERENCE POSTER

Mesenchymal stem-cell derived antimicrobial peptides for antimicrobial resistance @conference{9ff8c7ceb80c48fdb001962b4edda4da, title = "Mesenchymal stem-cell derived antimicrobial peptides for antimicrobial resistance", abstract = "Antimicrobial resistance (AMR) is a critical global health threat driven by antibiotic misuse and overuse, facilitating the emergence of resistant microorganisms [1]. Various mechanisms, including resistance gene spread, enzymatic modifications, and biofilm formation, contribute to multidrug-resistant bacteria. As antibiotic efficacy declines, infection control becomes challenging, increasing treatment failure rates [1]. AMR is particularly concerning in sepsis, a life-threatening condition caused by a dysregulated host response to infection. Excessive inflammation and immune suppression trigger systemic inflammatory responses, leading to cytokine overproduction, cascade activation, and multiorgan dysfunction [2]. Given this scenario, antimicrobial peptides (AMPs) present a promising alternative to conventional antibiotics. Their broad-spectrum activity and immunomodulatory properties set them apart from traditional antibiotics, which target specific bacterial pathways. AMPs act through membrane disruption, inhibition of nucleic acid and protein synthesis, immune modulation, and biofilm prevention [3,4].", author = "Silva, \{Mariana J.\} and Gustavo Fehrenbach and Patrick Murray and Emanuele Rezoagli and Laffey, \{John G.\} and Murphy, \{Emma J.\}", note = "Includes bibliographical references; TUS Research Week 2025 ; Conference date: 17-02-2025 Through 21-02-2025", year = "2025", month = feb, day = "18", language = "English (Ireland)", } . TUS Research Week 2025, Limerick, Ireland, 17/02/25.

Mesenchymal stem-cell derived antimicrobial peptides for antimicrobial resistance @misc { author = {Mariana J. Silva and Gustavo Fehrenbach and Patrick Murray and Emanuele Rezoagli and John G. Laffey and Emma J. Murphy}, title = {Mesenchymal stem-cell derived antimicrobial peptides for antimicrobial resistance}, year = {2025}, month = {feb} } .

Organ-on-chip for sepsis modeling @conference{bffcc031eaa34fbea91f0f37d064d3a1, title = "Organ-on-chip for sepsis modeling", abstract = "{"}Sepsis is one of the leading causes of mortality worldwide and is characterized by a life-threatening dysregulated host immune response. Effective disease modelling is critical for advancing diagnostic and therapeutic strategies. However, existing in vitro and in vivo models fail to capture the full spectrum of the disease, limiting their translational relevance. To address this gap, the present study aims to explore organ-on-chip technology as a novel model for sepsis, replicating the endothelial environment under physiological blood flow conditions and inducing sepsis using established methodologies. This approach may provide a more relevant system for disease mechanisms and organ interactions, potentially identifying candidates for therapeutic interventions{"}.", keywords = "Sepsis modeling, Organ-on-chip technology", author = "Silva, \{Mariana J.\} and Patrick Murray and Emanuele Rezoagli and Laffey, \{John G.\} and Emma Murphy and Gustavo Fehrenbach", year = "2025", month = feb, day = "18", language = "English (Ireland)", note = "RUN-EU Student Research Colloquium 2025 ; Conference date: 18-02-2025 Through 19-02-2025", } . RUN-EU Student Research Colloquium 2025, Limerick, Ireland, 18/02/25.

Organ-on-chip for sepsis modeling @misc { author = {Mariana J. Silva and Patrick Murray and Emanuele Rezoagli and John G. Laffey and Emma Murphy and Gustavo Fehrenbach}, title = {Organ-on-chip for sepsis modeling}, year = {2025}, month = {feb}, keywords = {Sepsis modeling; Organ-on-chip technology} } .

The effect of extreme salinity on the microalgae Dunaliella salina @conference{757920b402994b97b3cc1d0063b2988d, title = "The effect of extreme salinity on the microalgae Dunaliella salina", abstract = "This study looks at how D. salina reacts to different salinity levels, focusing on growth, pigment concentration and desalination potential. Out of 21 salinities, 7.5\% salinity produced consistently favourable results in cell count, dry biomass, pigment content, and desalination.", author = "Tanoeiro, \{Joao Rui\} and Gustavo Fehrenbach and Rui Pedrosa and Patrick Murray and Chen, \{Yuan Yuan\}", note = "Includes bibliographical references; TUS Research Week 2024 ; Conference date: 12-02-2024 Through 16-02-2024", year = "2024", month = feb, day = "12", language = "English (Ireland)", url = "https://tus.ie/rdi/research/tus-research-week/research-week-agenda/", } . TUS Research Week 2024, Athlone, Ireland, 12/02/24.

The effect of extreme salinity on the microalgae Dunaliella salina @misc { author = {Joao Rui Tanoeiro and Gustavo Fehrenbach and Rui Pedrosa and Patrick Murray and Yuan Yuan Chen}, title = {The effect of extreme salinity on the microalgae Dunaliella salina}, year = {2024}, month = {feb} } .

Developing sustainable freshwater acquaculture using Irish peatlands during COVID-19 crisis @misc { author = {Emer O'Neill and Gustavo Fehrenbach and Emma Murphy and Robert Pogue and Mark Lynch and Neil Rowan}, title = {Developing sustainable freshwater acquaculture using Irish peatlands during COVID-19 crisis}, year = {2020} } .

Gustavo Fehrenbach, Emer O&#39;Neill, Emma Murphy, Robert Pogue, Mark Lynch, Neil Rowan(2020). Developing sustainable freshwater acquaculture using Irish peatlands during COVID-19 crisis .

DISSERTATION THESIS

Development of pilot-scale treatment technologies for safeguarding seafood industry @phdthesis{9cb7883abb65442f9a0b29f00f21def0, title = "Development of pilot-scale treatment technologies for safeguarding seafood industry", abstract = "Seafood is a vital protein source whose consumption has doubled over the last 50 years, exceeding 20 kg per capita in 2014. Shellfish such as oysters and clams are seafood often consumed fresh or lightly cooked, which can lead to food intoxication if the shellfish accumulate contaminants from their environment. This is particularly true for filter-feeder species like oysters and mussels, which can filter several litres of water per hour. To improve the seafood and consumer safety, this PhD project aimed to develop and evaluate novel water purification alternatives in depuration plants for the seafood industry, mitigate seafood and human exposure to contaminants, support shellfish resilience and stress-mitigation, and develop prevention strategies through continuous monitoring of shellfish farms. An in-depth literature review addressing critical topics related to the PhD project was presented, including a comprehensive overview of the seafood industry, available and current water treatment technologies in depuration plants, water contaminants present in water that threatens seafood safety, and supplement-feed strategies to support oyster immune defence. Based on the literature review and the established objectives, scientific articles reporting the analysis and assessment of exposure to enteric viruses and bacteria in shellfish, application of shellfish as bioindicators of environmental contamination and their interactions with contaminants, and the implications for the seafood industry, consumers and the environment arising from contamination of shellfish were submitted to peer-reviewing process and published in scientific journals. Two novel water purification methods were developed: Pulsed ultraviolet light (PUV) water treatment - published in scientific journal, and electrocoagulation (EC) for water treatment in the seafood industry - an Invention Disclosure Form was submitted, and its patentability is under analysis. A last paper reporting the use of mushroom waste-derived bioactive sugars for oyster feeding to prevent and reduce the levels of contaminants in oysters, enhance sustainability, and productivity in the seafood industry was submitted for publication. Several key points were identified within this project, such as the need for prediction models and real time monitoring of exposure to contaminants, and not only developing water treatment techniques; a change in dietary habits to increase the ingestion of shellfish to health levels might increase the exposure potentially leading to increased intoxication incidents; E. coli by itself is not a reliable marker for food safety; shellfish are XVI susceptible to a wide diversity of contaminants and respond through biomarkers that can be used for an efficient monitoring of contaminants; a consensus on the requisite number and diversity of biomarkers for water quality and food safety assessment may support decision-making and assessing water quality in different regions; PUV and EC water treatment are promising alternatives to support shellfish purification facilities; beta-glucan extract obtained from upcycled mushroom can increase oyster resilience in contaminated environments and/or preserve quality while in depuration process. Future work will involve the installation of PUV and EC in real-scale depuration plants, implementation of monitoring strategies for continuous assessment of water conditions, and optimization of feed strategies. These goals will be pursued through subsequent PhD projects in collaboration with industry and university.", author = "Fehrenbach, \{Gustavo Waltzer\}", note = "Includes bibliographical references pp.172-205", year = "2024", month = may, day = "1", language = "English (Ireland)", publisher = "Technological University of the Shannon: Midlands Midwest", address = "Ireland", school = "Technological University of the Shannon", } .

Development of pilot-scale treatment technologies for safeguarding seafood industry @phdthesis { title = {Development of pilot-scale treatment technologies for safeguarding seafood industry}, year = {2024}, month = {may}, url = {https://research.tus.ie/en/publications/9cb7883a-bb65-442f-9a0b-29f00f21def0} } .