プロフィール詳細

プロジェクトを作成

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Dr. Khawar S.に依頼

Australia

I am a R&D scientist and teacher in the field of protein chemistry, protein-structure-function and enzyme biotechnology

プロフィール概要

I employ spectroscopic, calorimetric, electrophoretic, chromatographic bioinformatics and MS methods to characterize native, extremophilic and chemically-modified proteins/enzymes. Also taught enzymology, biology, protein chemistry/bioinformatics and methods in biochemistry courses to undergraduate, MS and M.Phil students. Interests include site-selective chemical modification to immobilize enzymes on conventional and nanomaterials. See https://scholar.google.com/citations?user=iyUkULcAAAAJ&hl=en for list of publications.

専門分野

• ★ Biotechnology & Bioengineering
• ☆ Protein/Enzyme Engineering

サービス

• Writing
• Research
• Data & AI

Writing Technical Writing

Research Gray Literature Search, Systematic Literature Review, Secondary Data Collection

Data & AI Statistical Analysis, Data Visualization

職務経験

Project Officer

University of New South Wales

11月 2019 - 現在

Project Officer

University of New South Wales

11月 2019 - 現在

Associate Professor

King Fahd University of Petroleum and Minerals (KFUPM), Dhahran, KSA

9月 2014 - 8月 2019

Senior Research Associate

University of New South Wales

2月 2001 - 7月 2014

Senior Scientific Officer

National Institute for Biotechnology & Genetic Engineering (NIBGE), Pakistan

1月 1990 - 9月 1999

学歴

PhD (Biochemistry)

Imperial College London

8月 1986 - 7月 1990

PhD

Imperial College, London, UK

8月 1986 - 11月 1989

Masters

Peshawar University, Pakistan

2月 1983 - 11月 1985

認定資格

• 認定資格の詳細は未入力です。

出版物

JOURNAL ARTICLE

(2019). Tuscan Varieties of Sweet Cherry Are Rich Sources of Ursolic and Oleanolic Acid: Protein Modeling Coupled to Targeted Gene Expression and Metabolite Analyses . Molecules (Basel, Switzerland).

(2019). Identification of the laccase-like multicopper oxidase gene family of sweet cherry (Prunus avium L.) and expression analysis in six ancient Tuscan varieties . Scientific reports.

(2019). Site-directed chemically-modified magnetic enzymes: fabrication, improvements, biotechnological applications and future prospects . Biotechnology advances.

(2018). Production of Plant Secondary Metabolites: Examples, Tips and Suggestions for Biotechnologists . Genes.

(2018). Novel Insights from Comparative In Silico Analysis of Green Microalgal Cellulases . International journal of molecular sciences.

(2018). How to calculate thermostability of enzymes using a simple approach . Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology.

(2018). Synthesis, Characterization and Applications of Magnetic Iron Oxide Nanostructures . Arabian Journal for Science and Engineering.

(2017). Identification of fasciclin-like arabinogalactan proteins in textile hemp (Cannabis sativa L.): in silico analyses and gene expression patterns in different tissues . BMC genomics.

(2017). Enhancement of lipase stability and productivity through chemical modification and its application to latex-based polymer emulsions . Process Biochemistry.

(2016). Characterization of a temperature-responsive two component regulatory system from the Antarctic archaeon, Methanococcoides burtonii . Scientific reports.

(2016). Defying the activity-stability trade-off in enzymes: taking advantage of entropy to enhance activity and thermostability . Critical reviews in biotechnology.

(2016). Single TRAM domain RNA-binding proteins in Archaea: functional insight from Ctr3 from the Antarctic methanogen Methanococcoides burtonii . Environmental microbiology.

(2016). Lignocellulosic biomass: Biosynthesis, degradation, and industrial utilization . Engineering in Life Sciences.

(2015). Some like it hot, some like it cold: Temperature dependent biotechnological applications and improvements in extremophilic enzymes . Biotechnology advances.

(2015). A new broad specificity alkaline metalloprotease from a Pseudomonas sp. isolated from refrigerated milk: Role of calcium in improving enzyme productivity . Journal of Molecular Catalysis B: Enzymatic.

(2015). Destructuring plant biomass: focus on fungal and extremophilic cell wall hydrolases . Plant science : an international journal of experimental plant biology.

(2014). Influence of calcium in extracellular DNA mediated bacterial aggregation and biofilm formation . PloS one.

(2014). Influence of calcium in extracellular DNA mediated bacterial aggregation and biofilm formation . PloS one.

(2014). Versatile peroxidase degradation of humic substances: use of isothermal titration calorimetry to assess kinetics, and applications to industrial wastes . Journal of biotechnology.

(2013). Psychrophiles . Annual Review of Earth and Planetary Sciences.

(2012). Kinetic and thermodynamic characterization of the functional properties of a hybrid versatile peroxidase using isothermal titration calorimetry: Insight into manganese peroxidase activation and lignin peroxidase inhibition . Biochimie.

(2012). Identification of two residues essential for the stringent substrate specificity and active site stability of the prokaryotic l-arginine:glycine amidinotransferase CyrA . The FEBS journal.

(2011). Chaperonins from an Antarctic archaeon are predominantly monomeric: crystal structure of an open state monomer . Environmental microbiology.

(2011). Biotechnological uses of enzymes from psychrophiles . Microbial Biotechnology.

(2010). The RNA polymerase subunits E/F from the Antarctic archaeon Methanococcoides burtonii bind to specific species of mRNA . Environmental microbiology.

(2010). A novel prokaryotic L-arginine:glycine amidinotransferase is involved in cylindrospermopsin biosynthesis . The FEBS journal.

(2010). A chemically modified alpha-amylase with a molten-globule state has entropically driven enhanced thermal stability . Protein engineering, design & selection : PEDS.

(2009). A novel approach for enhancing the catalytic efficiency of a protease at low temperature: reduction in substrate inhibition by chemical modification . Biotechnology and bioengineering.

(2009). The genome sequence of the psychrophilic archaeon, Methanococcoides burtonii: the role of genome evolution in cold adaptation . The ISME journal.

(2007). Structure and function of cold shock proteins in archaea . Journal of Bacteriology.

(2007). Kinetic and thermodynamic study of a chemically modified highly active xylanase from Scopulariopsis sp: existence of an essential amino group . Applied biochemistry and biotechnology.

(2006). Role of lysine versus arginine in enzyme cold-adaptation: modifying lysine to homo-arginine stabilizes the cold-adapted alpha-amylase from Pseudoalteramonas haloplanktis . Proteins.

(2006). Cold-adapted enzymes . Annual review of biochemistry.

(2005). The active site is the least stable structure in the unfolding pathway of a multidomain cold-adapted alpha-amylase . Journal of Bacteriology.

(2005). Role of disulfide bridges in the activity and stability of a cold-active alpha-amylase . Journal of Bacteriology.

(2005). Improved thermal stability and activity in the cold-adapted lipase B from Candida antarctica following chemical modification with oxidized polysaccharides . Extremophiles : life under extreme conditions.

(2005). Innovative kinetic and thermodynamic analysis of a purified superactive xylanase from Scopulariopsis sp . Applied biochemistry and biotechnology.

(2004). Improved activity and stability of alkaline phosphatases from psychrophilic and mesophilic organisms by chemically modifying aliphatic or amino groups using tetracarboxy-benzophenone derivatives. Cellular and molecular biology (Noisy-le-Grand, France).

(2004). A novel thermodynamic relationship based on Kramers Theory for studying enzyme kinetics under high viscosity . IUBMB life.

(2002). Thermodynamic activation properties of elongation factor 2 (EF-2) proteins from psychrotolerant and thermophilic Archaea . Extremophiles : life under extreme conditions.

(2002). Coupling of surface carboxyls of carboxymethylcellulase with aniline via chemical modification: extreme thermostabilization in aqueous and water-miscible organic mixtures . Biotechnology progress.

(2000). Carboxyl group modification significantly altered the kinetic properties of purified carboxymethylcellulase from Aspergillus niger . Enzyme and microbial technology.

(2000). [] . Biotechnology Letters.

(1999). Partial and complete alteration of surface charges of carboxymethylcellulase by chemical modification: thermostabilization in water-miscible organic solvent . Enzyme and Microbial Technology.

(1998). Carboxy-group modification: high-temperature activation of charge-neutralized and charge-reversed beta-glucosidases from Aspergillus niger. Biotechnology and applied biochemistry.

(1998). Thermodynamic and kinetic study of stability of the native and chemically modified β-glucosidases from Aspergillus niger . Process Biochemistry.

(1998). Automation of compartmental electrophoresis apparatus for the separation of non-covalently attached polysaccharides from proteins . Enzyme and Microbial Technology.

(1997). [] . Biotechnology Techniques.

(1997). [] . Biotechnology Letters.

(1996). Activity and thermostability of carboxymethylcellulase from Aspergillus niger is strongly influenced by noncovalently attached polysaccharides . World journal of microbiology & biotechnology.

(1996). The stability of extracellular beta-glucosidase from Aspergillus niger is significantly enhanced by non-covalently attached polysaccharides . Folia microbiologica.

(1994). A simple and nondestructive method for the separation of polysaccharides from beta-glucosidase produced extracellularly by Aspergillus niger . Enzyme and microbial technology.

(1993). Arthrobacter D-xylose isomerase: chemical modification of carboxy groups and protein engineering of pH optimum . The Biochemical journal.

(1993). Arthrobacter D-xylose isomerase: partial proteolysis with thermolysin . The Biochemical journal.

BOOK CHAPTER

(2017). Modular and Multifunctional Enzyme Systems for Plant Cell Wall Degradation: Diversity, Synergy, Chimeras and Magnetic-Glycosidases ☆ . Reference Module in Life Sciences.

(2017). Biotechnological Improvements of Cold-Adapted Enzymes: Commercialization via an Integrated Approach . Psychrophiles: From Biodiversity to Biotechnology.

(2006). 17 Proteins from Psychrophiles . Methods in Microbiology.