プロフィール詳細

プロジェクトを作成

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Lauren D.に依頼

United Kingdom

Molecular Biologist

プロフィール概要

Post-doctoral researcher with skills in fungal genetics, genomics, NGS sequencing, RNA biology, machine learning and bioinformatics

専門分野

• ☆ Microbiology
• ☆ Mycology/Fungi
• ☆ Molecular Biology
• ☆ Omics
• ☆ Genomics
• ☆ Next Generation Sequencing (NGS)
• ☆ Data Analysis
• ☆ Machine Learning
• ☆ R
• ☆ Infectious Disease

サービス

• Writing
• Research
• Consulting
• Data & AI

Writing Technical Writing, General Proofreading & Editing

Research Scientific and Technical Research, Systematic Literature Review

Consulting Scientific and Technical Consulting

Data & AI Predictive Modeling, Statistical Analysis, Data Visualization, Data Cleaning

職務経験

Research Associate

University of Kent

6月 2025 - 現在

Post doctoral research scholar

University of North Carolina at Charlotte

6月 2023 - 現在

Research Technician

University of Manchester

1月 2018 - 1月 2023

学歴

PhD, Infectious Diseases (Infection, Genomics and Evolution)

University of Manchester

9月 2018 - 3月 2023

認定資格

• 認定資格の詳細は未入力です。

出版物

PREPRINT

Lauren Dineen, Bryan Zavala, Kaitlin J Fisher, Dana A Opulente, Marie-Claire Harrison, John F Wolters, Xing-Xing Shen, Xiaofan Zhou, Marizeth Groenewald, Chris Todd Hittinger, et al. (2024). Genomic factors shaping codon usage across the Saccharomycotina subphylum .

Lauren Dineen, Bryan Zavala, Kaitlin J. Fisher, Dana A. Opulente, Marie-Claire Harrison, John F. Wolters, Xing-Xing Shen, Xiaofan Zhou, Marizeth Groenewald, Chris Todd Hittinger, et al. (2024). Genomic factors shaping codon usage across the Saccharomycotina subphylum .

JOURNAL ARTICLE

Lauren Dineen, Ana Cristina Colabardini, Norman Van Rhijn, Abigail L. LaBella, Clara Valero, Antonis Rokas, Gustavo H. Goldman(2022). Aspergillus fumigatus FhdA Transcription Factor Is Important for Mitochondrial Activity and Codon Usage Regulation during the Caspofungin Paradoxical Effect . Antimicrobial Agents and Chemotherapy. 66. (9). American Society for Microbiology

Fast and reliable PCR amplification from Aspergillus fumigatus spore suspension without traditional DNA extraction @article{35301013c6e1418dbf356d76f0c67759, title = "Fast and reliable PCR amplification from Aspergillus fumigatus spore suspension without traditional DNA extraction", abstract = "Aspergillus fumigatus is an opportunistic human pathogenic mould. DNA extraction from this fungus is usually performed by mechanical perturbation of cells as it possesses a rigid and complex cell wall. While this is not problematic for single isolates, it can be time consuming for large numbers of strains by using traditional DNA extraction procedures. Therefore, in this protocol we describe a fast and efficient thermal shock method to release DNA from spores of A. fumigatus and other filamentous fungi without the need of complex extraction methods. This is especially important for high-throughput PCR analyses of mutants such as in 96 or 384 well formats in a very short period of time without any concern of sample cross-contamination. This protocol is currently being used to validate the protein coding gene and non-coding RNA knock-out libraries in A. fumigatus generated in our laboratory and could be used in the future for diagnostics purposes.", keywords = "Aspergillus fumigatus, spore PCR, DNA extraction, filamentous fungi", author = "Marcin Fraczek and Can Zhao and Lauren Dineen and Ressa Lebedinec and Paul Bowyer and Michael Bromley and Daniela Delneri", year = "2019", month = aug, day = "20", doi = "10.1002/cpmc.89", language = "English", journal = "Current Protocols in Microbiology ", issn = "1934-8533", publisher = "John Wiley \& Sons Ltd", } . Current Protocols in Microbiology.

High throughput gene replacement in Aspergillus fumigatus @article{a121a6105f23426a91b453e13cefeee5, title = "High throughput gene replacement in Aspergillus fumigatus", abstract = "Aspergillus fumigatus is a human pathogen and the principal etiologicalagent of invasive and chronic aspergillosis leading to several hundreds ofthousands of deaths every year. Very few antifungals are available to treat infections caused by A. fumigatus and resistance is developing to those we have. Our understanding of the molecular mechanisms that drive pathogenicity and drug resistance have been hampered by the lack of large mutant collections which limit our ability to perform functional genomics analysis. Here we present a high-throughput gene knockout method that combines a highly reproducible fusion PCR method to enable generation of gene replacement cassettes with a multi-well format transformation procedure. This process can be used to generate96 null mutants within 5 days by a single person at a cost of less than £18 (\$24) per mutant and is being employed in our laboratory to generate a barcoded genome-wide knockout library in A. fumigatus.", author = "Can Zhao and Marcin Fraczek and Lauren Dineen and Ressa Lebedinec and Juliane Macheleidt and Thorsten Heinekamp and Daniela Delneri and Paul Bowyer and Brakhage, \{Axel A.\} and Michael Bromley", year = "2019", month = aug, day = "7", doi = "10.1002/cpmc.88", language = "English", journal = "Current Protocols in Microbiology ", issn = "1934-8533", publisher = "John Wiley \& Sons Ltd", } . Current Protocols in Microbiology.