プロフィール詳細

プロジェクトを作成

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Dr. Michelle G.に依頼

Mexico

PhD : Expert in Virology, Vaccines, biotherapeutics production and genetic editing.

プロフィール概要

Goal-oriented scientist with over 25 years of experience, passionate about transforming knowledge into tangible solutions for global health. Holder of a PhD in Biochemical Sciences and postdoctoral experience at the Institute of Biotechnology, UNAM. Specialized in recombinant vaccines, monoclonal antibodies, and viral mechanisms, integrating advanced techniques such as CRISPR to design innovative protocols for genome editing and biotechnological production. Previous research projects: Genetic characterization and stability of a CHO clone producing monoclonal antibodies. Increased productivity through molecular methods, vector design, and generation of recombinant CHO lines using CRISPR technology. Construction of molecular vectors for biologics expression in CHO cells Recombinant hemagglutinin (rHA) expression in CHO cells as an influenza vaccine. Production and purification of recombinant proteins (SARS-CoV-2 RBD, influenza HA, ZIKA NS1, lentiviral particles). Key Achievements: Design and validation of genetic editing protocols applied to viral models and to the improvement of recombinant protein production. Coordination of projects with impact in both pharmaceutical industry and academia. Mentorship of students who have continued into graduate programs and research lines. Creation of outreach materials reaching hundreds of people across Mexico. Publications in Journal of Virology, PNAS, Scientific Reports, Infection, International Journal of Environmental Research and Public Health, Future Virology among others. Skills Cell Biology: Mammalian, insect, and bacterial cell culture; stable cell line development; toxicity assays. Virology: Virus propagation, neutralization, titration, hemagglutination, viral particle purification and labeling. Molecular Biology: DNA/RNA purification, cloning, transfection, siRNA/esiRNA silencing, PCR/qPCR, plasmid design, genome editing, NGS. Immunology: Immunofluorescence, Western blot, immunoprecipitation, ELISA, serological assays. Protein Biochemistry: Expression, purification, FPLC/HPLC, SDS-PAGE, tangential filtration.

専門分野

• ★ Molecular Biology
• ★ Drug Development
• ★ Vaccine Development
• ☆ PCR
• ☆ Recombinant DNA
• ☆ Plasmid DNA
• ☆ Cell Biology
• ☆ Cell Culture
• ☆ Pulmonology/Respiratory Disease
• ☆ Influenza
• ☆ Medical Sciences

サービス

• Writing
• Product Development

Writing Clinical Trial Documentation, Medical Writing, Non-Medical Regulatory Writing, Technical Writing, Copywriting, Creative Writing

Product Development Recipe Development

職務経験

Posdoctoral researcher

Universidad Nacional Autónoma de México Instituto de Biotecnología

8月 2017 - 現在

Posdoctoral researcher

Universidad Nacional Autónoma de México Instituto de Biotecnología

11月 2021 - 12月 2025

Postdoctoral Researcher SECIHTI

Universidad Nacional Autonoma de México Instituto de Biotecnología

9月 2023 - 9月 2025

Project Leader artiaBIO, USA

Universidad Nacional Autonoma de México

8月 2017 - 1月 2020

学歴

PhD in Biochemistry (Virology)

Universidad Nacional Autónoma de México Instituto de Biotecnología

8月 2005 - 2月 2011

認定資格

• 10th Course on Vaccine Technology

  European Society for the Advancement of Cell Technologies

  10月 2025 - 現在

• Infection prevention and control measures in health-care settings for Ebola or Marburg disease outbreaks

  World Health Organization

  12月 2024 - 現在

• Avian and other zoonotic influenza: Introduction

  World Health Organization

  5月 2024 - 現在

• Tenth Course on Animal Cell Technology

  European Society of Animal Cell Technology

  9月 2019 - 現在

出版物

MAGAZINE ARTICLE

Alcala,A.C., Barreto,D., Contreras,M., Gutierrez,M., Hernandez,V., Pastor,A.R. (2020). Aportaciones del laboratorio de Ingeniería de Bioprocesos y Nanobiotecnología frente a la pandemia de COVID19. Biotecnologia, 24 (3), 54-60.

Gutierrez,M., Lopez,S. (2010). Mecanismos de entrada de virus: Una manera de conocer a la céula. TIP revista especializada en Ciencias Quimico-Biologicas, 13 (1), 26-34.

JOURNAL ARTICLE

E. Mendez and C. Munoz-Yanez and C. Sanchez-San Martin and G. Aguirre-Crespo and M. d. R. Banos-Lara and M. Gutierrez and R. Espinosa and Y. Acevedo and C. F. Arias and S. Lopez and R. M. Sandri-Goldin(2014). Characterization of Human Astrovirus Cell Entry . Journal of Virology. 88. (5). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 2452--2460. American Society for Microbiology

D. Silva-Ayala and T. Lopez and M. Gutierrez and N. Perrimon and S. Lopez and C. F. Arias(2013). Genome-wide RNAi screen reveals a role for the ESCRT complex in rotavirus cell entry . Proceedings of the National Academy of Sciences. 110. (25). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 10270--10275. Proceedings of the National Academy of Sciences

Jos{\'{e}} J Carre{\~{n}}o-Torres and Michelle Guti{\'{e}}rrez and Carlos F Arias and Susana L{\'{o}}pez and Pavel Isa(2010). Characterization of viroplasm formation during the early stages of rotavirus infection . Virology Journal. 7. (1). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 350. Springer Science and Business Media {LLC}

Michelle Gutie{\'}rrez and Pavel Isa and Claudia Sa{\'}nchez-San Martin and Jimena Pe{\'}rez-Vargas and Rafaela Espinosa and Carlos F. Arias and Susana Lo{\'}pez(2010). Different Rotavirus Strains Enter MA104 Cells through Different Endocytic Pathways: the Role of Clathrin-Mediated Endocytosis . Journal of Virology. 84. (18). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 9161--9169. American Society for Microbiology

Pavel Isa and Michelle Guti{\'{e}}rrez and Carlos F Arias and Susana L{\'{o}}pez(2008). Rotavirus cell entry . Future Virology. 3. (2). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 135--146. Future Medicine Ltd