プロフィール詳細

プロジェクトを作成

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Dr. Mohamed G.に依頼

Egypt

Biophysics & Molecular Biology Consultant | Single-Molecule Imaging, Protein Science, Assay Development

プロフィール概要

I am a Ph.D. biophysicist with over 10 years of research experience in the USA, UK and Japan, specializing in single-molecule biophysics, molecular biology, protein biochemistry, and advanced fluorescence microscopy. My expertise focuses on understanding biomolecular interactions, protein–nucleic acid recognition, and conformational dynamics using cutting-edge single-molecule techniques. I obtained my Ph.D. in Biophysics from the University of Illinois at Urbana-Champaign, USA, where I used multi-color single-molecule total internal reflection fluorescence microscopy (TIRFM) to directly investigate the relationship between protein conformational dynamics and DNA repair mechanisms. During my postdoctoral research at Imperial College London, UK, I studied nucleosome remodeling mechanisms using single-molecule FRET (smFRET). As a Research Scientist at Saint Louis University, USA, I applied advanced single-molecule approaches, including correlative optical trapping–fluorescence microscopy and ALEX smFRET, to study DNA repair and homologous recombination proteins. My technical expertise includes: • Single-molecule fluorescence microscopy (TIRFM, smFRET, ALEX-smFRET) • Correlative optical trapping–fluorescence microscopy • Molecular cloning and site-directed mutagenesis • Recombinant protein expression and purification • Protein biochemistry and functional assays • Quantitative analysis of single-molecule and fluorescence data Beyond research, I have a good teaching experience in Biophysics, Molecular Biology, Biochemistry, and Physics. I have taught undergraduate and graduate courses as a lecturer at Cairo University, Egypt, and served as a Teaching Assistant at the University of Illinois at Urbana-Champaign, USA. I can support research and industry projects involving: • Experimental design and optimization • Molecular and biophysical characterization of proteins and nucleic acids • Fluorescence microscopy and imaging strategies • Single-molecule data analysis and interpretation • Scientific consulting, literature review, and technical writing • Training and mentoring in biophysics and molecular biology techniques With international research experience across leading institutions in the USA, UK and Japan, I bring a combination of advanced experimental expertise, scientific problem-solving skills, and strong communication abilities to collaborative research and consulting projects.

専門分野

• ☆ Biochemistry
• ☆ Biochemical Assays
• ☆ Enzyme Activity Assays
• ☆ Binding Assays
• ☆ Molecular Biology
• ☆ Chromatin
• ☆ PCR
• ☆ Molecular Cloning
• ☆ Recombinant DNA
• ☆ Plasmid DNA
• ☆ DNA Damage & Repair
• ☆ Biophysics
• ☆ Microscopy
• ☆ Light Microscopy
• ☆ Fluorescence Microscopy

サービス

• Writing
• Research
• Consulting
• Data & AI

Writing Medical Writing, Technical Writing, General Proofreading & Editing, Translation

Research User Research, Technology Scouting, Fact Checking, Scientific and Technical Research, Systematic Literature Review

Consulting Go-to-Market Strategy Consulting, Scientific and Technical Consulting

Data & AI Statistical Analysis, Data Visualization

職務経験

Senior Researcher

University of Kansas

9月 2025 - 現在

Research Instructor

University of Nebraska Medical Centre

12月 2025 - 5月 2026

Research Scientist

Saint Louis University

5月 2024 - 8月 2025

Research Scientist

Saint Louis University

5月 2024 - 8月 2025

Postdoctoral researcher

University of Colorado, Anschutz Medical Campus

12月 2019 - 4月 2024

Postdoctoral researcher

MRC / Imperial College London

2月 2017 - 10月 2019

Postdoctoral researcher

Harvard University

9月 2015 - 5月 2016

学歴

Bachelor of Science (Biophysics)

Cairo University

- 現在

Ph.D

University of Illinois at Urbana-Champaign

8月 2008 - 5月 2015

Doctoral (Ph.D.) (Biophysics)

University of Illinois at Urbana-Champaign

8月 2008 - 5月 2015

Master of Sceince (Chemistry)

Kyoto University

4月 2004 - 3月 2006

B.Sc.

Cairo university, Egypt

9月 1995 - 5月 1999

認定資格

• Philosophy Doctorate of Science

  University of Illinois at Urbana-Champaign

  5月 2015 - 現在

出版物

JOURNAL ARTICLE

Mohamed Ghoneim, Rahul Chadda, Sibes Bera, Tamara De Melo, Duane P Grandgenett, Edwin Antony, Krishan K Pandey (2026). Delineation of a novel assembly intermediate in Rous sarcoma virus integration pathway . Nucleic Acids Research.

Mohamed Ghoneim, Jaigeeth Deveryshetty, Ayush Mistry, Sushil Pangeni, Monica Tokmina-Lukaszewska, Steven K. Gore, Jie Liu, Vikas Kaushik, Simrithaa Karunakaran, Angela Taddei, et al. (2025). Mechanism of Rad51 filament formation by Rad52 and Rad55-Rad57 in homologous recombination . Nature Communications.

Mohamed Ghoneim, Catherine A. Musselman (2023). Protocol to prepare doubly labeled fluorescent nucleosomes for single-molecule fluorescence microscopy . STAR Protocols.

Mohamed Ghoneim, Catherine A. Musselman (2023). Single-Molecule Characterization of Cy3.5 -Cy5.5 Dye Pair for FRET Studies of Nucleic Acids and Nucleosomes . Journal of Fluorescence.

Mohamed Ghoneim, Harrison A. Fuchs, Catherine A. Musselman (2021). Histone Tail Conformations: A Fuzzy Affair with DNA . Trends in Biochemical Sciences.

Mohamed Ghoneim, Joseph Tibbs, Colleen C Caldwell, Troy Buzynski, Wayne Bowie, Elizabeth M Boehm, M Todd Washington, S M Ali Tabei, Maria Spies(2021). KERA: analysis tool for multi-process, multi-state single-molecule data . Nucleic Acids Research. Oxford University Press ({OUP})

(2019). Retroviral integration into nucleosomes through DNA looping and sliding along the histone octamer . Nature Communications.

(2019). Selective deployment of transcription factor paralogs with submaximal strength facilitates gene regulation in the immune system . Nature Immunology.

(2018). Structure and dynamics of the yeast SWR1-nucleosome complex . Science.

Mohamed Ghoneim, Oliver Willhoft, Chia-Liang Lin, Eugene Y. D. Chua, Martin Wilkinson, Yuriy Chaban, Rafael Ayala, Elizabeth A. McCormack, Lorraine Ocloo, David S. Rueda, et al.(2018). Structure and dynamics of the yeast SWR1-nucleosome complex . Science. 362. (6411). American Association for the Advancement of Science ({AAAS})

(2014). Direct Correlation of DNA Binding and Single Protein Domain Motion via Dual Illumination Fluorescence Microscopy . Nano Letters.

(2007). A line-scanning semi-confocal multi-photon fluorescence microscope with a simultaneous broadband spectral acquisition and its application to the study of the thylakoid membrane of a cyanobacterium Anabaena PCC7120 . Journal of Microscopy.

BOOK CHAPTER

(2016). Quantifying the Assembly of Multicomponent Molecular Machines by Single-Molecule Total Internal Reflection Fluorescence Microscopy . Single-Molecule Enzymology: Fluorescence-Based and High-Throughput Methods.

(2013). Structure and Mechanisms of SF2 DNA Helicases . Advances in Experimental Medicine and Biology.

Three-Dimensional High-Resolution Microspectroscopic Study of Environment-Sensitive Photosynthetic Membranes . Molecular Nano Dynamics.