プロフィール詳細

プロジェクトを作成

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☆☆☆☆☆

Nadia P.に依頼

United Kingdom

Recombinant Protein Expression, Purification, Challenging Target Optimisation & Production Workflow Development

プロフィール概要

I have extensive experience developing protein production strategies for diverse protein families and new research targets, from construct design and high-throughput expression screening through to scale-up, purification and biophysical characterisation. I developed and implemented high-throughput workflows, including 5 mL expression screening approaches, and supported the transfer of successful methods into reproducible workflows used by wider research teams. My expertise includes recombinant protein expression, particularly in E. coli, and purification using Cytiva ÄKTA chromatography systems, including affinity, ion exchange, mixed-mode and size exclusion chromatography. I have experience optimising approaches for technically challenging proteins, including large, multimeric, cysteine-rich, metal-binding and aggregation-prone targets, using structured troubleshooting approaches to improve yield, quality and reproducibility. Alongside hands-on laboratory expertise, I have supported research teams by advising on expression strategies, purification approaches, experimental design and workflow improvements. I have developed biochemical and biophysical characterisation workflows, including high-throughput differential scanning fluorimetry (DSF) and nuclease detection assays, and have experience with enzyme assays, fluorescence-based assays and analytical size exclusion chromatography. I also have experience supporting laboratory operations, including CL2 laboratory management, equipment selection, procurement, SOP development, risk assessments and mentoring junior scientists. I enjoy helping teams overcome technical challenges and translating research requirements into practical, reliable laboratory solutions

専門分野

• ☆ Biochemistry
• ☆ Biochemical Assays
• ☆ Structural Biology
• ☆ Enzyme Activity Assays
• ☆ Binding Assays
• ☆ Molecular Biology
• ☆ PCR
• ☆ Molecular Cloning
• ☆ Next Generation Sequencing (NGS)
• ☆ Recombinant DNA
• ☆ Plasmid DNA
• ☆ Biotechnology & Bioengineering
• ☆ Recombinant Protein Expression
• ☆ Microbial Biotechnology
• ☆ Microbial Fermentation
• ☆ Blotting

サービス

• Writing
• Research

Writing Technical Writing

Research Technology Scouting, Fact Checking, Scientific and Technical Research

職務経験

Senior Scientist

Oxford Nanopore Technologies

9月 2007 - 12月 2025

Research Technician

Structural Genomics Consortium

4月 2005 - 9月 2007

Research Scientists

Oxitec

10月 2002 - 4月 2005

Graduate Research Assistant / Research Assistant

University of Oxford

10月 1999 - 9月 2002

学歴

Msc

University of Oxford

10月 1990 - 6月 1993

Bsc Applied Biology

University of East London

10月 1985 - 6月 1988

認定資格

• 認定資格の詳細は未入力です。