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プロフィール詳細
プロジェクトを作成
★★★★★
☆☆☆☆☆
Nadzeya S.に依頼
Netherlands

Proteomics & mass spectrometry PhD, scientific writing, manuscript editing, content creation, data analysis

プロフィール概要
専門分野
サービス
Writing Clinical Trial Documentation, Medical Writing, Non-Medical Regulatory Writing, Technical Writing, Copywriting, Creative Writing, General Proofreading & Editing, Translation
Research Market Research, User Research, Fact Checking, Gap Analysis, Scientific and Technical Research, Systematic Literature Review
Consulting Business Strategy Consulting, Healthcare Consulting, Scientific and Technical Consulting, Regulatory Consulting
Data & AI Statistical Analysis, Data Visualization, Big Data Analytics, Data Cleaning, Data Processing, Data Insights
Product Development Product Evaluation, Product Validation, Quality Assurance & Control (QA/QC)
職務経験

Universiteit Utrecht

- 現在

PhD Scientist

Utrecht University

9月 2022 - 現在

Head of Pharmacy, Pharmacist

Farmatsiya

9月 2014 - 8月 2020

学歴

PhD (Biomolecular Mass Spectrometry and Proteomics)

Universiteit Utrecht

9月 2022 - 現在

MSc (Drug Innovation)

Utrecht University

9月 2020 - 8月 2022

Pharmacy

Vitebsk State Medical University

9月 2007 - 6月 2011

認定資格
出版物
JOURNAL ARTICLE
Spore inoculum size impacts substrate degradation and sporulation but not the secretome during colonization of whole yellow pea (Pisum sativum) by Aspergillus oryzae @article{c7c8be9622f74403a2047e3b2dc41850, title = "Spore inoculum size impacts substrate degradation and sporulation but not the secretome during colonization of whole yellow pea (Pisum sativum) by Aspergillus oryzae", abstract = "Aspergillus oryzae is used in solid-state fermentation (SSF) to produce plant-based foods. To this end, the substrate is inoculated with spores of this fungus. So far, the effect of inoculum size on SSF with A. oryzae has primarily focused on the production of specific enzymes . Therefore, the aim of this study was to examine the impact of inoculum size on the full secretome, combined with enzyme activity assays, assessment of colonization, substrate degradation, and sporulation. To this end, A. oryzae was grown for 7 days on whole yellow pea ( Pisum sativum ). Fluorescence microscopy with a GFP-expressing A. oryzae strain showed that peas had been colonized externally and internally, irrespective of inoculum size. Yet, the highest inoculum size resulted in a stronger pea biomass reduction when compared to the lowest inoculum size. By contrast, sporulation decreased with increasing inoculum size. Notably, proteomics revealed no effect of inoculum size on the protein profiles of aqueous extracts of the colonized peas. Amylases and proteases were the most abundant secreted proteins, which was consistent with their high activity in the aqueous extracts. Proteomics also identified β-1,3-glucanases and chitinases, indicating hyphal lysis. Indeed, 10–19\% of the fungal proteins detected in the aqueous extracts lacked signal peptides. These data contribute to our understanding of colonization of substrates by A. oryzae and may be used to optimize SSF with this food grade fungus.", keywords = "Aspergillus oryzae, Fungus, Pea, Pisum sativum, Plant-based meat alternative, Solid-state fermentation", author = "Maaike Braat and Nienke Braat and Nadzeya Staliarova and Tom{\'a}s Vellozo-Echevarr{\'i}a and Siwek, \{Hania M.\} and Kemmer, \{Julius F.\} and Kristian Barrett and Meyer, \{Anne S.\} and Stecker, \{Kelly E.\} and W{\"o}sten, \{Han A.B.\}", note = "Publisher Copyright: {\textcopyright} 2026 The Authors.", year = "2026", month = sep, day = "2", doi = "10.1016/j.ijfoodmicro.2026.111853", language = "English", volume = "458", journal = "International Journal of Food Microbiology", issn = "0168-1605", publisher = "Elsevier BV", } . International Journal of Food Microbiology.
Nadzeya Staliarova, Maaike Braat, Nienke Braat, Tomás Vellozo-Echevarría, Hania M. Siwek, Julius F. Kemmer, Kristian Barrett, Anne S. Meyer, Kelly E. Stecker, Han A.B. Wösten (2026). Spore inoculum size impacts substrate degradation and sporulation but not the secretome during colonization of whole yellow pea (Pisum sativum) by Aspergillus oryzae . International Journal of Food Microbiology.
Nadzeya Staliarova, Matteo Boretto, Maarten H. Geurts, Shashank Gandhi, Ziliang Ma, Martina Celotti, Sangho Lim, Gui-Wei He, Rosemary Millen, Else Driehuis, et al. (2024). Epidermal growth factor receptor (EGFR) is a target of the tumor-suppressor E3 ligase FBXW7 . Proceedings of the National Academy of Sciences.
Epidermal growth factor receptor (EGFR) is a target of the tumor-suppressor E3 ligase FBXW7 @article{b58772429efd4c81aac70414604b6891, title = "Epidermal growth factor receptor (EGFR) is a target of the tumor-suppressor E3 ligase FBXW7", abstract = "FBXW7 is an E3 ubiquitin ligase that targets proteins for proteasome-mediated degradation and is mutated in various cancer types. Here, we use CRISPR base editors to introduce different FBXW7 hotspot mutations in human colon organoids. Functionally, FBXW7 mutation reduces EGF dependency of organoid growth by \textasciitilde{}10,000-fold. Combined transcriptomic and proteomic analyses revealed increased EGFR protein stability in FBXW7 mutants. Two distinct phosphodegron motifs reside in the cytoplasmic tail of EGFR. Mutations in these phosphodegron motifs occur in human cancer. CRISPR-mediated disruption of the phosphodegron motif at T693 reduced EGFR degradation and EGF growth factor dependency. FBXW7 mutant organoids showed reduced sensitivity to EGFR-MAPK inhibitors. These observations were further strengthened in CRC-derived organoid lines and validated in a cohort of patients treated with panitumumab. Our data imply that FBXW7 mutations reduce EGF dependency by disabling EGFR turnover.", keywords = "colorectal cancer, EGFR, FBXW7, organoids", author = "Matteo Boretto and Geurts, \{Maarten H.\} and Shashank Gandhi and Ziliang Ma and Nadzeya Staliarova and Martina Celotti and Sangho Lim and He, \{Gui Wei\} and Rosemary Millen and Else Driehuis and Harry Begthel and Lidwien Smabers and Jeanine Roodhart and \{van Es\}, Johan and Wei Wu and Hans Clevers", note = "Publisher Copyright: Copyright {\textcopyright} 2024 the Author(s). Published by PNAS.", year = "2024", month = mar, day = "19", doi = "10.1073/pnas.2309902121", language = "English", volume = "121", journal = "Proceedings of the National Academy of Sciences of the United States of America", issn = "0027-8424", publisher = "National Academy of Sciences", number = "12", } . Proceedings of the National Academy of Sciences of the United States of America.
Human fetal brain self-organizes into long-term expanding organoids @article{7beb7a9cb91a4a6d922ae5b9e6fb544e, title = "Human fetal brain self-organizes into long-term expanding organoids", abstract = "Human brain development involves an orchestrated, massive neural progenitor expansion while a multi-cellular tissue architecture is established. Continuously expanding organoids can be grown directly from multiple somatic tissues, yet to date, brain organoids can solely be established from pluripotent stem cells. Here, we show that healthy human fetal brain in vitro self-organizes into organoids (FeBOs), phenocopying aspects of in vivo cellular heterogeneity and complex organization. FeBOs can be expanded over long time periods. FeBO growth requires maintenance of tissue integrity, which ensures production of a tissue-like extracellular matrix (ECM) niche, ultimately endowing FeBO expansion. FeBO lines derived from different areas of the central nervous system (CNS), including dorsal and ventral forebrain, preserve their regional identity and allow to probe aspects of positional identity. Using CRISPR-Cas9, we showcase the generation of syngeneic mutant FeBO lines for the study of brain cancer. Taken together, FeBOs constitute a complementary CNS organoid platform.", keywords = "brain cancer, brain development, CRISPR-Cas9, ECM, human fetal brain, morphogens, organoids, regional identity, tissue culture, tumor modeling", author = "Delilah Hendriks and Anna Pagliaro and Francesco Andreatta and Ziliang Ma and \{van Giessen\}, Joey and Simone Massalini and Carmen L{\'o}pez-Iglesias and \{van Son\}, \{Gijs J.F.\} and Jeff DeMartino and Damen, \{J. Mirjam A.\} and Iris Zoutendijk and Nadzeya Staliarova and Bredenoord, \{Annelien L.\} and Holstege, \{Frank C.P.\} and Peters, \{Peter J.\} and Thanasis Margaritis and \{Chuva de Sousa Lopes\}, Susana and Wei Wu and Hans Clevers and Benedetta Artegiani", note = "Publisher Copyright: {\textcopyright} 2023 The Author(s)", year = "2024", month = feb, day = "1", doi = "10.1016/j.cell.2023.12.012", language = "English", volume = "187", pages = "712--732", journal = "Cell", issn = "0092-8674", publisher = "Elsevier BV", number = "3", } . Cell.
Nadzeya Staliarova, Delilah Hendriks, Anna Pagliaro, Francesco Andreatta, Ziliang Ma, Joey van Giessen, Simone Massalini, Carmen López-Iglesias, Gijs J.F. van Son, Jeff DeMartino, et al. (2024). Human fetal brain self-organizes into long-term expanding organoids . Cell.
Nadzeya Staliarova, Gui-Wei He, Lin Lin, Jeff DeMartino, Xuan Zheng, Talya Dayton, Harry Begthel, Willine J. van de Wetering, Eduard Bodewes, Jeroen van Zon, et al. (2022). Optimized human intestinal organoid model reveals interleukin-22-dependency of paneth cell formation . Cell Stem Cell.