プロフィール詳細

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Dr. Nishikant W.に依頼

United States

Freelance Statistician, Data Analyst & Mass Spectrometry Leader| Expert in R with 14+ years of experience

プロフィール概要

Hey there! I’m Nishikant Wase, a mass spectrometry pro with 12+ years of expertise across different cutting-edge platforms LC-MS/GC-MS (Thermo Orbitrap IDX, Exploris, Steller MS, Sciex TripleQuad 6500, 7500 MS/MS systems, Bruker and Waters HRMS Instruments) and GC-MS (Agilent GC-TOF and triple quad GC-MS) systems—ready to turbocharge your R&D projects! I’ve designed and validated top-notch ADC methods for GLP studies, mastered metabolomics, lipidomics, and proteomics (TMT, iTRAQ), peptide mapping analysis. Worked on more than 100 + projects and delivered 40+ impactful publications, including in Nature Communications, Nature Metabolism and Plant Physiology. With hands-on skills in Agilent/Thermo instruments and data wizardry in R, I bring actionable insights and collaborative leadership to solve your toughest challenges. Let’s team up to turn your data into breakthroughs—contact me today and let’s make it happen!

専門分野

• ★ Analytical Chemistry
• ★ Mass Spectrometry
• ★ Preclinical ADME/PK/PD
• ☆ Liquid Chromatography/HPLC
• ☆ Peptide Synthesis
• ☆ Biochemistry
• ☆ Molecular Biology
• ☆ Omics
• ☆ Proteomics
• ☆ Metabolomics
• ☆ Next Generation Sequencing (NGS)
• ☆ Data Analysis
• ☆ Data Processing
• ☆ Renewable Energy
• ☆ Biomarker Discovery
• ☆ Protein/Enzyme Engineering
• ☆ Drug Development
• ☆ Small Molecule Drug Development
• ☆ Biologic & Biosimilar Development
• ☆ Drug Substance Characterization
• ☆ Bioanalytical Testing
• ☆ Preclinical Research
• ☆ Drug Efficacy Testing
• ☆ In vitro Diagnostics
• ☆ Biomarker Analysis
• ☆ Metabolism & Metabolic Disease
• ☆ Research

サービス

• Writing
• Research
• Consulting
• Data & AI
• Product Development

Writing Medical Writing, Non-Medical Regulatory Writing, Technical Writing

Research Feasibility Study, Scientific and Technical Research, Systematic Literature Review

Consulting Scientific and Technical Consulting

Data & AI Statistical Analysis, Data Visualization, Big Data Analytics, Text Mining & Analytics, Data Mining, Data Cleaning, Data Processing, Data Insights

Product Development Product Validation

職務経験

Senior Scientist

University of Virginia

1月 2020 - 現在

Senior Research Associate

University of Nebraska-Lincoln

5月 2011 - 12月 2019

Assistant Professor

Dr. Ambedkar College

1月 2005 - 9月 2006

Assistant Professor

Nabira Mahavidyalaya

6月 2003 - 1月 2005

Lecturer

Rajaram College

9月 2002 - 4月 2003

学歴

PhD (Chemical and Biological Engineering)

University of Sheffield

10月 2006 - 4月 2011

MSc (Post Graduate Department of Botany)

Rashtrasant Tukadoji Maharaj Nagpur University

1997 - 1999

認定資格

• 認定資格の詳細は未入力です。

出版物

JOURNAL ARTICLE

Wase, Nishikant, Vijayan, Jithesh, Alvarez, Sophie, Naldrett, Michael J., Morse, Wyatt, Maliva, Amanda, Riekhof, Wayne R. (2024). Nitrogen starvation leads to TOR kinase-mediated downregulation of fatty acid synthesis in the algae <i>Chlorella sorokiniana</i> and <i>Chlamydomonas reinhardtii</i> . BMC Plant Biology.

Wase, Nishikant, Vijayan, Jithesh, Liu, Kan, Morse, Wyatt, Zhang, Chi, Riekhof, Wayne R. (2024). ROS-mediated thylakoid membrane remodeling and triacylglycerol biosynthesis under nitrogen starvation in the alga <i>Chlorella sorokiniana</i> . Frontiers in Plant Science.

Wase, Nishikant, Vijayan, Jithesh, Alvarez, Sophie, Naldrett, Michael J, Maliva, Amanda, Riekhof, Wayne R (2023). Nitrogen starvation leads to TOR kinase-mediated downregulation of fatty acid synthesis in the algae Chlorella sorokiniana and Chlamydomonas reinhardtii . BioRxiv.

Wase, Nishikant, Iker Etchegaray, J., Kelley, Shannon, Penberthy, Kristen, Karvelyte, Laura, Nagasaka, Yosuke, Gasperino, Sofia, Paul, Soumen, Seshadri, Vikram, Raymond, Michael, et al. (2023). Phagocytosis in the retina promotes local insulin production in the eye . Nature Metabolism.

Nishikant Wase, Guangchao Sun, Shengqiang Shu, Jerry Jenkins, Bangjun Zhou, J. Vladimir Torres-Rodr&#237;guez, Cindy Chen, Laura Sandor, Chris Plott, Yuko Yoshinga, et al. (2022). Genome of Paspalum vaginatum and the role of trehalose mediated autophagy in increasing maize biomass . Nature Communications.

Nishikant Wase(2022). Longitudinal Metabolomics and Lipidomics Analyses Reveal Alterations Associated with Envenoming by Bothrops asper and Daboia russelii in an Experimental Murine Model . Toxins. 14. (10). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 657. {MDPI} {AG}

Longitudinal Metabolomics and Lipidomics Analyses Reveal Alterations Associated with Envenoming by Bothrops asper and Daboia russelii in an Experimental Murine Model <head> <META HTTP-EQUIV="Refresh" CONTENT="0;URL=/servlet/useragent"> </head> . Toxins.

L. R. Dillard and N. Wase and G. Ramakrishnan and J. J. Park and N. E. Sherman and R. Carpenter and M. Young and A. N. Donlan and W. Petri and J. A. Papin(2022). Leveraging metabolic modeling to identify functional metabolic alterations associated with COVID-19 disease severity . Metabolomics. 18. (7). Springer Science and Business Media {LLC}

Nishikant Wase, Nathan Abshire, Toshihiro Obata (2022). High-Throughput Profiling of Metabolic Phenotypes Using High-Resolution GC-MS . Methods in Molecular Biology.

Wase, Nishikant, Gutierrez, Jose Maria, Rucavado, Alexandra, Fox, Jay W. (2022). Longitudinal Metabolomics and Lipidomics Analyses Reveal Alterations Associated with Envenoming by Bothrops asper and Daboia russelii in an Experimental Murine Model . Toxins.

Paul Manka and Svenja Sydor and Nishikant Wase and Jan Best and Malte Brandenburg and Annika Hellbeck and Julia Schänzer and Ramiro Vilchez-Vargas and Alexander Link and Anja Figge and Andreas Jähnert and Ulrike von Arnim and Jason D Coombes and Francisco Javier Cubero and Alisan Kahraman and Moon-Sung Kim and Julia Kälsch and Sonja Kinner and Klaas Nico Faber and Han Moshage and Guido Gerken and Wing-Kin Syn and Scott L Friedman and Ali Canbay and Lars P Bechmann(2021). Anti‐TNFα treatment in Crohn’s disease: Impact on hepatic steatosis, gut‐derived hormones, and metabolic status . Liver International. Wiley

Laura J. Dunphy and Kassandra L. Grimes and Nishikant Wase and Glynis L. Kolling and Jason A. Papin(2021). Untargeted Metabolomics Reveals Species-Specific Metabolite Production and Shared Nutrient Consumption by Pseudomonas aeruginosa and Staphylococcus aureus . mSystems. 6. (3). American Society for Microbiology

Nishikant Wase, Guangchao Sun, Shengqiang Shu, Jerry Jenkins, Bangjun Zhou, Cindy Chen, Laura Sandor, Chris Plott, Yuko Yoshinga, Christopher Daum, et al. (2021). The genome of stress tolerant crop wild relative Paspalum vaginatum leads to increased biomass productivity in the crop Zea mays . BioRxiv.

Nishikant Wase, Vincent M. Perez, Jeffrey Gabell, Mark Behrens, Concetta C. DiRusso, Paul N. Black(2020). Deletion of fatty acid transport protein 2 (FATP2) in the mouse liver changes the metabolic landscape by increasing the expression of PPARα-regulated genes . Journal of Biological Chemistry. 295. (17). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 5737--5750. Elsevier {BV}

(2019). Transcriptome analysis-identified long noncoding RNA CRNDE in maintaining endothelial cell proliferation, migration, and tube formation . Scientific Reports.

Nishikant Wase, Boqiang Tu, Girish Kumar Rasineni, Ronald Cerny, Ryan Grove, Jiri Adamec, Paul N. Black, Concetta C. DiRusso(2019). Remodeling of Chlamydomonas Metabolism Using Synthetic Inducers Results in Lipid Storage during Growth . Plant Physiology. 181. (3). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 1029--1049. American Society of Plant Biologists ({ASPB})

Wase, Nishikant, Moran, Matthew, Cheng, Xiao, Ali, Mohamed Sham Shihabudeen Haider, Nghi Nguyen, Yang, Weilong, Zhang, Chi, DiRusso, Concetta, Sun, Xinghui (2019). Transcriptome analysis-identified long noncoding RNA CRNDE in maintaining endothelial cell proliferation, migration, and tube formation . Scientific Reports.

Bhinderwala F, Wase N, DiRusso C, Powers R(2018). Combining Mass Spectrometry and NMR Improves Metabolite Detection and Annotation . Journal of proteome research.

Nishikant Wase, Paul Black, Concetta DiRusso (2018). Innovations in improving lipid production: Algal chemical genetics . Progress in Lipid Research.

Wase, Nishikant, Black, Paul, DiRusso, Concetta (2018). Innovations in improving lipid production: Algal chemical genetics . Progress in Lipid Research.

Wase, Nishikant, Bhinderwala, Fatema, DiRusso, Concetta, Powers, Robert (2018). Combining Mass Spectrometry and NMR Improves Metabolite Detection and Annotation . Journal of Proteome Research.

Nishikant Wase, Boqiang Tu, James W. Allen, Paul N. Black, Concetta C. DiRusso(2017). Identification and Metabolite Profiling of Chemical Activators of Lipid Accumulation in Green Algae . Plant Physiology. 174. (4). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 2146--2165. American Society of Plant Biologists ({ASPB})

Nishikant Wase, Boqiang Tu, James W Allen, Paul N Black, Concetta C DiRusso(2017). Identification and metabolite profiling of chemical activators of lipid accumulation in green algae . Plant Physiology. Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString pp.00433.2017. American Society of Plant Biologists ({ASPB})

Wase, Nishikant, Tu, Boqiang, Allen, James W., Black, Paul N., DiRusso, Concetta C. (2017). Identification and Metabolite Profiling of Chemical Activators of Lipid Accumulation in Green Algae . Plant Physiology.

Nishikant Wase, Boqiang Tu, Paul N. Black, Concetta C. DiRusso(2015). Phenotypic screening identifies Brefeldin A/Ascotoxin as an inducer of lipid storage in the algae Chlamydomonas reinhardtii . Algal Research. 11. Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 74--84. Elsevier {BV}

Wase, Nishikant, Tu, Boqiang, Black, Paul N., DiRusso, Concetta C. (2015). Phenotypic screening identifies Brefeldin A/Ascotoxin as an inducer of lipid storage in the algae Chlamydomonas reinhardtii . Algal Research.

Nishikant Wase, Paul N. Black, Bruce A. Stanley, Concetta C. DiRusso(2014). Integrated Quantitative Analysis of Nitrogen Stress Response in Chlamydomonas reinhardtii Using Metabolite and Protein Profiling . J. Proteome Res. 13. (3). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 1373â??1396. American Chemical Society (ACS)

Wase, N., Black, P.N., Stanley, B.A., Dirusso, C.C.(2014). Integrated quantitative analysis of nitrogen stress response in Chlamydomonas reinhardtii using metabolite and protein profiling . Journal of Proteome Research. 13. (3). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 1373-1396.

(2014). Integrated Quantitative Analysis of Nitrogen Stress Response in <i>Chlamydomonas reinhardtii</i> Using Metabolite and Protein Profiling . Journal of Proteome Research.

(2014). Discovery of small molecule hyperlipid accumulating triggers of algae Chlamydomonas reinhardtii identified via in vivo phenotype based screen . The FASEB Journal.

Wase, N., Pham, T.K., Ow, S.Y., Wright, P.C.(2014). Quantitative analysis of UV-A shock and short term stress using iTRAQ, pseudo selective reaction monitoring (pSRM) and GC-MS based metabolite analysis of the cyanobacterium Nostoc punctiforme ATCC 29133 . Journal of Proteomics. 109. Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 332-355.

Wase, Nishikant, Trong Khoa Pham, Ow, Saw Yen, Wright, Philip C. (2014). Quantitative analysis of UV-A shock and short term stress using iTRAQ, pseudo selective reaction monitoring (pSRM) and GC-MS based metabolite analysis of the cyanobacterium Nostoc punctiforme ATCC 29133 . Journal of Proteomics.

Nishikant V Wase, Saw Yen Ow, Malinda Salim, Mikkel Nissum, Mike Whalley (2012). A Free Flow Electrophoresis Separation Strategy for Segregation of High Abundant Phycobilisomes from Cyanobacterium Nostoc punctiforme PCC 73102 . Journal of Proteomics & Bioinformatics.

Wase, N.V., Ow, S.Y., Salim, M., Nissum, M., Whalley, M., Wright, P.C.(2012). A free flow electrophoresis separation strategy for segregation of high abundant phycobilisomes from cyanobacterium nostoc punctiforme PCC 73102 . Journal of Proteomics and Bioinformatics. 5. (3). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 67-72.

Nishikant Wase (2008). Systems biology of cyanobacterial secondary metabolite production and its role in drug discovery . Expert Opinion in Drug Discovery.

Wase, N.V., Wright, P.C.(2008). Systems biology of cyanobacterial secondary metabolite production and its role in drug discovery . Expert Opinion on Drug Discovery. 3. (8). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 903-929.

(2008). Systems biology of cyanobacterial secondary metabolite production and its role in drug discovery . Expert Opinion on Drug Discovery.

A global understanding of light stress in cyanobacteria: Environmental and bioproducts perspectives .

PREPRINT

Nishikant Wase, Jithesh Vijayan, Sophie Alvarez, Michael J Naldrett, Amanda Maliva, Wayne R. Riekhof (2023). Nitrogen starvation leads to TOR kinase-mediated downregulation of fatty acid synthesis in the algaeChlorella sorokinianaandChlamydomonas reinhardtii .

Reactivation of Crohn’s colitis in an adolescent following serial autologous fecal transplants for diversion colitis: A case report with multiomic characterization @article{PPR:PPR435413, title = {Reactivation of Crohn’s colitis in an adolescent following serial autologous fecal transplants for diversion colitis: A case report with multiomic characterization}, author = {Polanco EG and Murach M and Hoffman CL and Oliphant SL and Wase N and McGowan EC and Mendoza MV and Moore SR}, doi = {10.21203/rs.3.rs-1193197/v1} } .

Guangchao Sun and Nishikant Wase and Shengqiang Shu and Jerry Jenkins and Bangjun Zhou and Cindy Chen and Laura Sandor and Chris Plott and Yuko Yoshinga and Christopher Daum and Peng Qi and Kerrie Barry and Anna Lipzen and Luke Berry and Thomas Gottilla and Ashley Foltz and Huihui Yu and Ronan O'Malley and Chi Zhang and Katrien M. Devos and Brandi Sigmon and Bin Yu and Toshihiro Obata and Jeremy Schmutz and James C. Schnable(2021). The genome of stress tolerant crop wild relative Paspalum vaginatum leads to increased biomass productivity in the crop Zea mays . []. Cold Spring Harbor Laboratory

BOOK CHAPTER

Nishikant Wase(2022). High-Throughput Profiling of Metabolic Phenotypes Using High-Resolution GC-MS . Methods in Molecular Biology. Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 235--260. Springer {US}

OTHER

Nishikant Wase, Jithesh Vijayan, Kan Liu, Chi Zhang, Wayne R. Riekhof(2021). Reactive oxygen species mediate thylakoid membrane remodeling and triacylglycerol synthesis under nitrogen starvation in the alga Chlorella sorokiniana . Cold Spring Harbor Laboratory

Nishikant Wase, Saw Yen, Phillip Wright(2013). A Global Understanding of Light Stress in Cyanobacteria . Stress Biology of Cyanobacteria. Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 273â??290. CRC Press

DATA SET

(2017). Label-free quantification of proteome changes in Chlamydomonas reinhardtii treated with lipid inducing small molecules .

PATENT

Compounds for Increasing Lipid Synthesis and Storage .

CONFERENCE ABSTRACT

Nishikant Wase Discovery of small molecule hyperlipid accumulating triggers of algae Chlamydomonas reinhardtii identified via in vivo phenotype based screen .