プロフィール詳細

プロジェクトを作成

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Dr. Sandeep Kaur R.に依頼

United States

Scientist with 11 years of experience in molecular biology & biochemistry. Structural biologist in training. Team work.

プロフィール概要

I did my doctorate from CSIR-Imtech, Chandigarh, India and pursuing postdoctoral training at Purdue University, West Lafayette, United States. For my PhD thesis project, I worked on PknA, an essential eukaryotic-type serine/threonine kinase from Mycobacterium tuberculosis. I investigated role of juxta membrane domain towards its catalytic activity and phosphorylation mediated activation mechanism of PknA. This required me to clone, express and purify the protein and its variants from Escherichia coli. The purified catalytic module of PknA was used for structural determination using X-ray crystallography which gave us mechanistic understanding of PknA activation. Biochemical and biophysical techniques including kinase assays, western blotting and circular dichroism were utilized to further characterize the proteins. The findings from this study were published in FEBS journal. My research interests involve using structural biology and biochemical techniques to gain understanding as how dysregulated proteins cause disease. In postdoctoral period, I investigated regulation of P-Rex1, a guanine nucleotide exchange factor over-expressed in different cancers. I determined the structure of its regulatory domain, DEP1 using X-ray crystallography and elucidated its role in autoinhibition using fluorescence based enzymatic assays, Small Angle X-ray scattering and liposome sedimentation assays.

専門分野

• ★ Molecular Biology
• ☆ Cell Signaling Pathways
• ☆ Protein/Enzyme Engineering

サービス

• Research

Research Fact Checking, Gray Literature Search, Systematic Literature Review

職務経験

Postdoctoral Research Associate

Purdue University

8月 2017 - 現在

学歴

Bachelors in Science (Biotechnology)

Panjab University

7月 2002 - 6月 2005

認定資格

• 認定資格の詳細は未入力です。

出版物

JOURNAL ARTICLE

Yadav, G.S., Ravala, S.K., Kachhap, S., Thakur, M., Roy, A., Singh, B., Karthikeyan, S., Chakraborti, P.K.(2017). Eukaryotic-type serine/threonine kinase mediated phosphorylation at Thr¹⁶⁹ perturbs mycobacterial guanylate kinase activity . Bioscience Reports. 37. (6).

Yadav, G.S., Ravala, S.K., Malhotra, N., Chakraborti, P.K.(2016). Phosphorylation modulates catalytic activity of mycobacterial sirtuins . Frontiers in Microbiology. 7. (MAY).

Ravala, S.K., Singh, S., Yadav, G.S., Kumar, S., Karthikeyan, S., Chakraborti, P.K.(2015). Evidence that phosphorylation of threonine in the GT motif triggers activation of PknA, a eukaryotic-type serine/threonine kinase from Mycobacterium tuberculosis . FEBS Journal. 282. (8). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 1419-1431.