プロフィール詳細

プロジェクトを作成

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Dr. SIVAN KALYANI V.に依頼

India

CRISPR gRNA design for indel, prime edit, base edit, HDR donor template, cloning module for multiplex edit, mutant seq

プロフィール概要

Experienced researcher specializing in genome editing and molecular biology, currently working at the M S Swaminathan Research Foundation, India, my expertise lies in advancing CRISPR-based technologies for plant trait improvement and molecular innovation. Over the past six years, I have built significant technical expertise in designing and executing CRISPR-mediated gene editing experiments, including molecular cloning, plant transformation (Agrobacterium and biolistic methods), tissue culture, and advanced genomic analyses. During my tenure at Gyeongsang National University, Korea, I developed a CRISPR-Cas12a Gemini viral multi-replicon system that enhanced HDR-based genome editing efficiency in tomato, contributing to two US patents focused on small-molecule–mediated HDR improvements. At Iwate University, Japan, I employed CRISPR-Cas9 approaches to dissect gene functions in tomato development and stress responses, while my work in Agricultureal Research Organisation, Vulkani, Israel integrated transcriptomics and metabolomics to develop treatments that mitigate postharvest chilling injury in mango and avocado.

専門分野

• ★ Plant Physiology
• ★ Plant Biotechnology
• ★ Gene Editing/CRISPR
• ☆ Phytochemistry
• ☆ Biochemistry
• ☆ Agronomy & Crop Science
• ☆ Biomolecular Engineering

サービス

• Writing
• Research
• Consulting
• Data & AI

Writing Clinical Trial Documentation, Technical Writing, Copywriting, Creative Writing, General Proofreading & Editing

Research Technology Scouting, Fact Checking, Scientific and Technical Research, Systematic Literature Review, Secondary Data Collection

Consulting Scientific and Technical Consulting, Regulatory Consulting

Data & AI Predictive Modeling, Image Processing, Image Analysis, Data Visualization

職務経験

Co-Principal Investigator/ Senior Scientist

M S SWAMINATHAN RESEARCH FOUNDATION

11月 2022 - 現在

Specially Appointed Researcher

Iwate Daigaku

7月 2019 - 現在

Specially Appointed Researcher

Iwate University

6月 2019 - 12月 2020

Research Professor

Gyeongsang National University

7月 2016 - 4月 2018

Postdoctoral Researcher

Agricultural Research Organisation, VulKani Institute

1月 2014 - 12月 2015

学歴

Ph.D., in Biotechnology

Bharathiar University, Coimbatore, Tamil Nadu, India

1月 2008 - 12月 2013

MSc in Biotechnology

Bharathiar University, Coimbatore, Tamil Nadu, India

6月 2004 - 7月 2006

Bsc Biochemistry

Madurai Kamaraj University, Tamil Nadu, India

6月 2001 - 7月 2004

認定資格

• CSIR-JRF cum NET, Govt of India All India Rank 089

  CSIR, Govt of India

  6月 2011 - 6月 2011

出版物

JOURNAL ARTICLE

Sivan Kalyani Velu, Bhavini Krishnan, Gayatri Venkataraman (2025). Realizing the yield potential of Narrow Leaf 1 (NAL1) in rice: The way forward . Plant Physiology and Biochemistry.

Tien Van Vu, Velu Sivankalyani, Eun‐Jung Kim, Duong Thi Hai Doan, Mil Thi Tran, Jihae Kim, Yeon Woo Sung, Minwoo Park, Yang Jae Kang, Jae‐Yean Kim(2020). Highly efficient homology‐directed repair using CRISPR/Cpf1‐geminiviral replicon in tomato . Plant Biotechnology Journal. 18. (10). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 2133--2143. Wiley

Sivankalyani, V., Maoz, I., Feygenberg, O., Maurer, D., Alkan, N.(2017). Chilling stress upregulates a-linolenic acid-oxidation pathway and induces volatiles of C6 and C9 aldehydes in mango fruit . Journal of Agricultural and Food Chemistry. 65. (3). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 632-638.

Sivankalyani, V., Feygenberg, O., Diskin, S., Wright, B., Alkan, N.(2016). Increased anthocyanin and flavonoids in mango fruit peel are associated with cold and pathogen resistance . Postharvest Biology and Technology. 111. Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 132-139.

Sivankalyani, V., Sela, N., Feygenberg, O., Zemach, H., Maurer, D., Alkan, N.(2016). Transcriptome dynamics in mango fruit peel reveals mechanisms of chilling stress . Frontiers in Plant Science. 7. (OCTOBER2016).

Sivankalyani, V., Geetha, M., Subramanyam, K., Girija, S.(2015). Ectopic expression of Arabidopsis RCI2A gene contributes to cold tolerance in tomato . Transgenic Research. 24. (2). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 237-251.

Sivankalyani, V., Feygenberg, O., Maorer, D., Zaaroor, M., Fallik, E., Alkan, N.(2015). Combined treatments reduce chilling injury and maintain fruit quality in avocado fruit during cold quarantine . PLoS ONE. 10. (10).

Sivankalyani, V., Takumi, S., Thangasamy, S., Ashakiran, K., Girija, S.(2014). Punctured-hypocotyl method for high-efficient transformation and adventitious shoot regeneration of tomato . Scientia Horticulturae. 165. Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 357-364.