プロフィール詳細

プロジェクトを作成

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Dr. Spoorthy P.に依頼

Canada

Biomedical Researcher | PhD in Oncology | 7+ yrs Experience in Scientific Writing, Editing, Data Analysis & Lite

プロフィール概要

Biomedical researcher with a PhD in Cancer Radiation Biology and 7+ years of experience in oncology, radiobiology, and molecular biology. Proven expertise in scientific writing, data analysis, research consulting, and academic editing. Passionate about translating complex scientific concepts into accessible language for technical and non-technical audiences. Currently supporting global clients in medical writing, peer review, research design, and data interpretation. Open to freelance projects in biomedical sciences, cancer research, clinical data interpretation, and scientific content creation.

専門分野

• ☆ Cell Biology
• ☆ Cell-Based Assays
• ☆ Cytogenetics
• ☆ Cell Signaling Pathways
• ☆ Membrane Biology
• ☆ Gene Regulation
• ☆ Stem Cell Research
• ☆ Neuroscience
• ☆ Neurophysiology
• ☆ Visual Neuroscience
• ☆ Behavioral Neuroscience
• ☆ Cognitive Neuroscience
• ☆ Developmental Neuroscience
• ☆ Molecular Neuroscience
• ☆ Neurobiology
• ☆ Systems Neuroscience
• ☆ Mycology
• ☆ Marine Biology
• ☆ Biophysics
• ☆ Histology
• ☆ Biotechnology & Bioengineering
• ☆ Biomedical Engineering
• ☆ Microscopy
• ☆ Cryosectioning
• ☆ Light Microscopy
• ☆ Electron Microscopy
• ☆ Fluorescence Microscopy
• ☆ Scanning Probe Microscopy
• ☆ Cell Culture
• ☆ Cell Surface Receptors
• ☆ 3D Cell Culture
• ☆ Synthetic Biology
• ☆ Biosensors
• ☆ Biomolecular Engineering
• ☆ Gene Synthesis
• ☆ Genetic Engineering
• ☆ Gene Editing/CRISPR
• ☆ Protein/Enzyme Engineering
• ☆ Antibody Engineering
• ☆ Gene Delivery
• ☆ Biological Taxonomy/Classification
• ☆ Circadian Rhythm Research
• ☆ Tissue Engineering
• ☆ Artificial Organs
• ☆ Cytometry
• ☆ Cell Aging
• ☆ Medical Technologies
• ☆ Assistive Technology
• ☆ Pharmacy
• ☆ Pharmacy Education
• ☆ Clinical Pharmacy
• ☆ Drug Discovery
• ☆ High Throughput Screening (HTS)
• ☆ Drug Target Identification
• ☆ Hit-to-Lead & Lead Optimization
• ☆ Phenotypic Assays
• ☆ High Content Screening/Imaging
• ☆ Medicinal Chemistry
• ☆ Drug Design
• ☆ Combinatorial Chemistry
• ☆ Drug Development
• ☆ Drug Formulation
• ☆ Small Molecule Drug Development
• ☆ Biologic & Biosimilar Development
• ☆ Drug Substance Characterization
• ☆ Biosafety Testing
• ☆ Drug Stability Testing
• ☆ Drug-Drug Interactions
• ☆ Preclinical Pharmacology
• ☆ Drug Efficacy Testing
• ☆ Antibiotic Development
• ☆ Vaccine Development
• ☆ Pharmaceutical Manufacturing
• ☆ Pharmaceutical Process Development
• ☆ Bioprocess Development
• ☆ Small Molecule Manufacturing
• ☆ Biologic & Biosimilar Manufacturing
• ☆ Health Technology Assessment
• ☆ E-Health & Telemedicine
• ☆ Immunotherapy
• ☆ Gene Therapy
• ☆ Rural Health
• ☆ Pharmaceutical Management
• ☆ Drug Delivery Technologies
• ☆ Liposomes
• ☆ Pharmaceutical Technology
• ☆ Medical Equipment & Supplies
• ☆ Healthcare Reimbursement

サービス

• Writing
• Research
• Consulting
• Data & AI
• Product Development

Writing Clinical Trial Documentation, Medical Writing, Non-Medical Regulatory Writing, Technical Writing, Copywriting, Creative Writing

Research Market Research, User Research, Meta-Research, Technology Scouting, Gap Analysis, Gray Literature Search, Scientific and Technical Research, Systematic Literature Review, Secondary Data Collection

Consulting Digital Strategy Consulting, Healthcare Consulting, Scientific and Technical Consulting, Regulatory Consulting

Data & AI Predictive Modeling, Statistical Analysis, Image Processing, Image Analysis, Data Visualization, Data Mining, Data Cleaning, Data Processing, Data Insights

Product Development Formulation, Recipe Development, Product Evaluation, Product Validation, Quality Assurance & Control (QA/QC), Product Compliance , Packaging Design, Prototyping

職務経験

Research Fellow

Sonaro

5月 2025 - 現在

Research Consultant

PreScouter Inc.

5月 2025 - 現在

Research Mentor

uMaster Global

1月 2025 - 現在

Postdoctoral Fellow

The University of Oklahoma

9月 2023 - 8月 2024

City University of Hong Kong

- 8月 2023

Post-doctoral Fellow

City University of Hong Kong

12月 2022 - 8月 2023

PhD candidate

City University of Hong Kong

9月 2018 - 11月 2022

学歴

City University of Hong Kong

- 8月 2023

PhD

City University of Hong Kong

9月 2018 - 11月 2022

MSc

Bangalore University

8月 2015 - 7月 2017

BSc

Bangalore University

8月 2012 - 7月 2015

認定資格

• IGNITE training program on start-up based entrepreneurship

  City University of Hong Kong

  11月 2022 - 現在

• Ionising Radiation & X-ray protection course

  University of Hong Kong

  10月 2018 - 現在

出版物

JOURNAL ARTICLE

Radiation-induced rescue effect on human breast carcinoma cells is regulated by macrophages @article{d219e45f74884e838b6aff532d37fdd0, title = "Radiation-induced rescue effect on human breast carcinoma cells is regulated by macrophages", abstract = "The susceptibility of cancer cells to DNA damages is influenced by their microenvironment. For example, unirradiated neighbors of irradiated cells can produce signals that reduce DNA damages. This phenomenon, known as Radiation-Induced Rescue Effect (RIRE), has profound implications on the efficacy of radiotherapy. Using bystander cells co-cultured with mock-irradiated cells as a control, we demonstrated, for the first time, two types of RIRE. Conditioned medium from na{\"i}ve by stander cells, i.e., cells not exposed to irradiated cells, could mitigate UV-induced DNA damages in human breast carcinoma MCF7 cells, as judged by phospho-H2AX and 53BP1 immunostaining. This protective effect could be further enhanced by the prior treatment of bystander cells with factors from UV-irradiated cells. We named the former effect “basal RIRE” and the latter “active RIRE” which were cell type-dependent. As bystanders, MCF7 showed a significant active RIRE, whereas THP1-derived macrophages showed a strong basal RIRE but no active RIRE. Interestingly, RIRE of macrophages could further be modulated by polarisation. The basal RIRE of macrophages was abolished by M1 polarisation, while M2 and Tumour Associated Macrophages (TAM) demonstrated pronounced basal and active RIRE. When mixtures of MCF7 cells and polarised macrophages were used as bystanders, the overall RIRE was dictated by macrophage phenotypes: RIRE was suppressed by M1 macrophages but significantly enhanced by M2 and TAM. This study shows a previously unappreciated role of the innate immune system in RIRE. Depending on polarised phenotypes, macrophages in the tumour microenvironment can interfere with the effectiveness of radiotherapy by adjusting the RIRE magnitudes. {\textcopyright} 2025 The Authors", keywords = "DNA damage, M1/M2 macrophages, Radiation-induced rescue effect (RIRE), Tumour-associated macrophages (TAMs), UV", author = "Spoorthy Pathikonda and Li Tian and Arava, {Clement Manohar} and Cheng, {Shuk Han} and Lam, {Yun Wah}", year = "2025", month = mar, doi = "10.1016/j.bbrep.2025.101936", language = "English", volume = "41", journal = "Biochemistry and Biophysics Reports", issn = "2405-5808", publisher = "Elsevier B.V.", } . Biochemistry and Biophysics Reports.

Therapeutic Values of Exosomes in Cosmetics, Skin Care, Tissue Regeneration, and Dermatological Diseases @article{0a0b9d83f26f41bfb0b60d5cebce9340, title = "Therapeutic Values of Exosomes in Cosmetics, Skin Care, Tissue Regeneration, and Dermatological Diseases", abstract = "Exosomes are small extracellular nanovesicles that are released by cells, and their potential has been explored for use in cosmetics, skin care, tissue regeneration, and dermatological diseases. The therapeutic value of exosomes lies in their ability to modulate the microenvironment of cells, regulate gene expression, and induce cell differentiation, which can have a positive impact on skin health. In terms of cosmetics, exosomes have been used to reduce wrinkles, improve skin texture and hydration, and enhance skin elasticity, as well as to reduce inflammation and damage caused by UV radiation. Furthermore, exosomes have been used to promote tissue regeneration in skin wounds and to treat dermatological diseases such as systemic lupus erythematosus, psoriasis, atopic dermatitis, systemic sclerosis, pigment regulation, vitiligo, and hair growth. In this review, the therapeutic value of exosomes in the field of cosmetics, skin care, tissue regeneration, and dermatological diseases, has been elaborated. The existing literature demonstrated that with further research, exosomes may become a viable therapeutic option for many skin conditions. {\textcopyright} 2023 by the authors.", keywords = "cosmetics, dermatological research, exosome, skin disease, tissue regeneration", author = "Abhimanyu Thakur and Disheet Shah and Deepika Rai and Parra, {Diana Carolina} and Spoorthy Pathikonda and Svetlana Kurilova and Alma Cili", year = "2023", month = apr, doi = "10.3390/cosmetics10020065", language = "English", volume = "10", journal = "Cosmetics", issn = "2079-9284", publisher = "Multidisciplinary Digital Publishing Institute (MDPI)", number = "2", } . Cosmetics.

Highly Sensitive and Cost-Effective Portable Sensor for Early Gastric Carcinoma Diagnosis @article{f9967ffd96434483a397d602121cf931, title = "Highly Sensitive and Cost-Effective Portable Sensor for Early Gastric Carcinoma Diagnosis", abstract = "Facile and efficient early detection of cancer is a major challenge in healthcare. Herein we developed a novel sensor made from a polycarbonate (PC) membrane with nanopores, followed by sequence-specific Oligo RNA modification for early gastric carcinoma diagnosis. In this design, the gastric cancer antigen CA72-4 is specifically conjugated to the Oligo RNA, thereby inhibiting the electrical current through the PC membrane in a concentration-dependent manner. The device can determine the concentration of cancer antigen CA72-4 in the range from 4 to 14 U/mL, possessing a sensitivity of 7.029 µAU−1mLcm−2 with a linear regression (R2) of 0.965 and a lower detection limit of 4 U/mL. This device has integrated advantages including high specificity and sensitivity and being simple, portable, and cost effective, which collectively enables a giant leap for cancer screening technologies towards clinical use. This is the first report to use RNA aptamers to detect CA72-4 for gastric carcinoma diagnosis.", keywords = "polycarbonate membrane, cancer antigen, Oligo RNA, cancer early detection, electrical current sensor", author = "Oo, {Saw Lin} and Shishir Venkatesh and Vaithinathan Karthikeyan and Arava, {Clement Manohar} and Spoorthy Pathikonda and Yu, {Peter K. N.} and Lau, {Terrence C. K.} and Xianfeng Chen and Roy, {Vellaisamy A. L.}", year = "2021", month = apr, doi = "10.3390/s21082639", language = "English", volume = "21", journal = "Sensors", issn = "1424-8220", publisher = "Multidisciplinary Digital Publishing Institute (MDPI)", number = "8", } . Sensors.

Role of PARP1 regulation in radiation-induced rescue effect @article{800d59972ffa4c7db8cbfce30346db10, title = "Role of PARP1 regulation in radiation-induced rescue effect", abstract = "Radiation-induced rescue effect (RIRE) in cells refers to the phenomenon where irradiated cells (IRCs) receive help from feedback signals produced by partnered bystander unirradiated cells (UIRCs) or from the conditioned medium (CM) that has previously conditioned the UIRCs. In the present work, we explored the role of poly (ADP-ribose) polymerase 1 (PARP1) regulation in RIRE and the positive feedback loop between PARP1 and nuclear factor-kappa-light-chain-enhancer of activated B cell (NF-κB) in RIRE using various cell lines, including HeLa, MCF7, CNE-2 and HCT116 cells. We first found that when the IRCs (irradiated with 2 Gy X-ray) were treated with CM, the relative mRNA expression levels of both tumor suppressor p53-binding protein 1 (53BP1) and PARP1, the co-localization factor between 53BP1 and γH2AX as well as the fluorescent intensity of PARP1 were reduced. We also found that IRCs treated with the PARP1 inhibitor, Olaparib (AZD2281) had a higher 53BP1 expression. These results illustrated that PARP1 was involved in RIRE transcriptionally and translationally. We further revealed that treatment of IRCs with CM together with Olaparib led to significantly lower mRNA expression levels and fluorescent intensities of NF-κB, while treatment of IRCs with CM together the NF-κB inhibitor BAY-11-7082 led to significantly lower mRNA expression levels as well as fluorescent intensities of PARP1. These results illustrated that PARP1 and NF-κB were involved in the positive feedback loop transcriptionally and translationally. Thus, the results supported the occurrence of a PARP1-NF-κB positive feedback loop in RIRE. The present work provided insights into potential exploitation of inhibition of PARP1 and/or the PARP1-NF-κB positive feedback loop in designing adjuncts to cancer radiotherapeutics.", keywords = "BAY-11-7082, radiation-induced rescue effect (RIRE), PARP1, NF-κB, TNF-α, Olaparib", author = "Spoorthy Pathikonda and Cheng, {Shuk Han} and Yu, {Kwan Ngok}", year = "2020", month = may, doi = "10.1093/jrr/rraa023", language = "English", volume = "61", pages = "352--367", journal = "Journal of Radiation Research", issn = "0449-3060", publisher = "Oxford University Press", number = "3", } . Journal of Radiation Research.

PREPRINT

Spoorthy Pathikonda, Li Tian, Shuk Han Cheng, Yun Wah Lam (2023). Radiation-induced rescue effect on human breast carcinoma cells is regulated by macrophages .