プロフィール詳細

プロジェクトを作成

★★★★★

☆☆☆☆☆

★★★★★

☆☆☆☆☆

Srinivas K.に依頼

United States

Scientific consulting services - Viral Vector (AAV & LVV) design, development & production, and Bioanalytical assays

プロフィール概要

In my Industry oriented roles, as a Lead Scientist, I successfully led multiple projects on the design and development of Viral Vectors such as Lentiviral Vectors (LVV) for CAR-T and AAV, Cell line & Vector engineering, Upstream & downstream process development, development of bioanalytical, immunological, and cell-based assays, such as qPCR, ddPCR, ELISA, Flow Cytometry, NGS & potency assays for characterization of LVV and AAV. These efforts resulted in scalable, high-titer vector production and reliable analytical platforms that supported both discovery and manufacturing. I have a PhD in Virology and over ten years of combined academic & Industrial experience in Viral Vectors, Virology, cell and gene therapy, Process development, and Bioanalytical assay development, including Next-Generation Sequencing technologies. Throughout my career, I have focused on advancing the science of Biotherapeutics and collaborative execution. Also, I worked extensively on the role of RNA modification pathways, such as RNA methylation, on the biology of various viruses, including HSV-1, Coronaviruses, Neurovirology, Latency & reactivation of HSV-1 in primary neurons, the development/Engineering of viral vectors (Lentiviral, Retroviral & Adenoviral vectors), and the development of various cutting-edge technologies.

専門分野

• ☆ Microbiology
• ☆ Virology
• ☆ Coronaviruses
• ☆ Adenoviruses
• ☆ Molecular Virology
• ☆ Microbiological Assays
• ☆ Biochemistry
• ☆ Immunoassay
• ☆ Biochemical Assays
• ☆ Apoptosis
• ☆ Molecular Biology
• ☆ Genetics
• ☆ Omics
• ☆ Transcriptomics
• ☆ Genomics
• ☆ Epigenetics
• ☆ PCR
• ☆ Molecular Cloning
• ☆ Next Generation Sequencing (NGS)
• ☆ Gene Expression Analysis
• ☆ Recombinant DNA
• ☆ RNA Sequencing
• ☆ Plasmid DNA
• ☆ Cell Biology
• ☆ Cell-Based Assays
• ☆ Cell Signaling Pathways
• ☆ Gene Regulation
• ☆ Neuroscience
• ☆ Molecular Neuroscience
• ☆ Neurobiology
• ☆ Biotechnology & Bioengineering
• ☆ Recombinant Protein Expression
• ☆ Microscopy
• ☆ Light Microscopy
• ☆ Fluorescence Microscopy
• ☆ Biomarker Discovery
• ☆ Cell Culture
• ☆ Primary Cells
• ☆ Cell Banking
• ☆ Cell Line Engineering
• ☆ Large Scale Cell Culture
• ☆ Biomolecular Engineering
• ☆ Genetic Engineering
• ☆ Gene Editing/CRISPR
• ☆ Gene Delivery
• ☆ Blotting
• ☆ Immunohistochemistry
• ☆ Cytometry

サービス

• Writing
• Research
• Consulting
• Data & AI
• Product Development

Writing Technical Writing

Research Scientific and Technical Research, Systematic Literature Review

Consulting Scientific and Technical Consulting, Manufacturing Consulting

Data & AI Statistical Analysis, Data Processing

Product Development Formulation, Quality Assurance & Control (QA/QC), Concept Development

職務経験

Co-Founder

Stealth Startup

1月 2025 - 現在

Senior Scientist

CHO Plus

8月 2024 - 10月 2024

Senior Scientist-I

64 X Bio

7月 2023 - 7月 2024

Scientist-III & Lead

Sangamo Therapeutics

7月 2022 - 6月 2023

Post-Doctoral Fellow

New York University Langone Medical Center

11月 2016 - 6月 2022

学歴

PhD

Sri Venkateswara University

6月 2006 - 5月 2012

認定資格

• 認定資格の詳細は未入力です。

出版物

JOURNAL ARTICLE

Leon Sylvester Sanders, Courtney E. Comar, Kalanghad Puthankalam Srinivas, Joseph Lalli, Mark Salnikov, Joy Lengyel, Peter Southern, Ian Mohr, Angus C. Wilson, Stephen A. Rice (2023). Herpes Simplex Virus-1 ICP27 Nuclear Export Signal Mutants Exhibit Cell Type-Dependent Deficits in Replication and ICP4 Expression . Journal of Virology.

Hui‐Lan Hu, Kalanghad P Srinivas, Shuoshuo Wang, Moses V Chao, Timothee Lionnet, Ian Mohr, Angus C Wilson, Daniel P Depledge, Tony T Huang(2022). Single‐cell transcriptomics identifies Gadd45b as a regulator of herpesvirus‐reactivating neurons . EMBO reports. 23. (2). {EMBO}

Kalanghad Puthankalam Srinivas, Daniel P. Depledge, Jonathan S. Abebe, Stephen A. Rice, Ian Mohr, Angus C. Wilson(2021). Widespread remodeling of the m 6 A RNA-modification landscape by a viral regulator of RNA processing and export . Proceedings of the National Academy of Sciences. 118. (30). Proceedings of the National Academy of Sciences

Hannah M. Burgess, Daniel P. Depledge, Letitia Thompson, Kalanghad Puthankalam Srinivas, Rebecca C. Grande, Elizabeth I. Vink, Jonathan S. Abebe, Wesley P. Blackaby, Alan Hendrick, Mark R. Albertella, et al.(2021). Targeting the m6A RNA modification pathway blocks SARS-CoV-2 and HCoV-OC43 replication . Genes & Development. 35. (13-14). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 1005--1019. Cold Spring Harbor Laboratory

Daniel P. Depledge, Kalanghad Puthankalam Srinivas, Tomohiko Sadaoka, Devin Bready, Yasuko Mori, Dimitris G. Placantonakis, Ian Mohr, Angus C. Wilson (2019). Direct RNA sequencing on nanopore arrays redefines the transcriptional complexity of a viral pathogen . Nature Communications.

Vipin Mohan Dan, Balaji Muralikrishnan, Rahul Sanawar, Vinodh J. S., Bhushan Bapusaheb Burkul, Kalanghad Puthankalam Srinivas, Asha Lekshmi, N. S. Pradeep, Syed G. Dastager, B. Santhakumari, et al.(2018). Streptomyces sp metabolite(s) promotes Bax mediated intrinsic apoptosis and autophagy involving inhibition of mTOR pathway in cervical cancer cell lines . Scientific Reports. 8. (1). Springer Nature

Kalanghad Puthankalam Srinivas, Remadevi Viji, Vipin Mohan Dan, Indira Sukumaran Sajitha, Rajappan Prakash, Puthan Valappil Rahul, Thankayyan R. Santhoshkumar, Subhadra Lakshmi, Madhavan Radhakrishna Pillai(2016). DEPTOR promotes survival of cervical squamous cell carcinoma cells and its silencing induces apoptosis through downregulating PI3K/AKT and by up-regulating p38 MAP kinase . Oncotarget. 7. (17). Impact Journals, {LLC}

M. Sreekanth Reddy, S. Kanakala, K. P. Srinivas, M. Hema, V. G. Malathi, P. Sreenivasulu(2013). Complete genome sequence of a new begomovirus associated with yellow mosaic disease of Hemidesmus indicus in India . Archives of Virology. 159. (5). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 1223--1228. Springer Nature

K. P. Srinivas, M. Sreekanth Reddy, M. Hema, P. Sreenivasulu(2013). Multiplex RT-PCR for parallel detection of three RNA viruses associated with stripe and mosaic diseases of sorghum in India . European Journal of Plant Pathology. 137. (2). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 211--215. Springer Nature

M. Padmavathi, K.P. Srinivas, Ch. V. Subba Reddy, B. Ramesh, K. Navodayam, J. Krishnaprasadji, P. Ratan Babu, P. Sreenivasulu(2011). Identification of a New Potyvirus Associated with Chlorotic Vein Banding Disease of Spathiphyllum spp., in Andhra Pradesh, India . The Plant Pathology Journal. 27. (1). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 33--36. Korean Society of Plant Pathology

K. P. Srinivas, Ch. V. Subba Reddy, B. Ramesh, P. Lava Kumar, P. Sreenivasulu(2010). Identification of a virus naturally infecting sorghum in India as Sugarcane streak mosaic virus . European Journal of Plant Pathology. 127. (1). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 13--19. Springer Nature

BOOK CHAPTER

(2020). Using Primary SCG Neuron Cultures to Study Molecular Determinants of HSV-1 Latency and Reactivation. Herpes Simplex Virus - Methods in Molecular Biology.

OTHER

Daniel P Depledge, Srinivas Kalanghad Puthankalam, Tomohiko Sadaoka, Devin Beady, Yasuko Mori, Dimitris Placantonakis, Ian Mohr, Angus Wilson(2018). Native RNA sequencing on nanopore arrays redefines the transcriptional complexity of a viral pathogen . Cold Spring Harbor Laboratory