プロフィール詳細

プロジェクトを作成

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Subbu K.に依頼

United States

Chemical and molecular biologist with 15+ years of experience in basic scientific research

プロフィール概要

I have more than fifteen years of research experience in the design, synthesis and evaluation of biologically active peptides and small molecules. I offer proficiency in peptide and small molecule synthesis, and analytical techniques including but not limited to HPLC, Mass Spectrometry and Fluorescence Microscopy. I am also skilled in various classical molecular biological techniques such as Western blot, gel electrophoresis, ELISA, PCR, and in vitro gene silencing. I have also conducted in vivo drug testing in small animals. I am trained in aseptic mammalian and bacterial cell culture and maintenance. I am self learned in molecular modeling and visualization softwares such as PyMOL and UCSF Chimera. I conduct established in vitro molecular biological experiments to study the effect of therapeutic drug leads in a biological system. I also perform in vivo dosing of said drug leads in mice models. My current postdoctoral research is focused on developing peptides and small molecules that modulate vascular angiogenesis with a particular focus on ocular and oncologic disorders. I have designed, screened and evaluated novel peptide drug leads that can modulate blood vessel growth by targeting Vascular Endothelial Growth Factor (VEGF), Tyrosine kinase with Ig and EGF homology domains 2 (TIE2), and Sodium-coupled Neutral Amino acid Transporter 1 (SNAT1 or SLC38A1) mediated angiogenesis. I have also identified and evaluated novel Thalidomide derived drug leads that possess anti-angiogenic and/or anti-myeloma activity. For my doctoral thesis, I, along with a team of scientist with varying backgrounds, developed a lipidated peptide platform to screen for ligands that improve GPCR function. We tested our modified peptide ligands in cell and animal-based assays and reported improved GPCR activity in peer-reviewed journals. For another project, we synthesized and evaluated the effect of selective fluorination on the biological activity of Glucagon-like peptide-1.

専門分野

• ★ Organic Chemistry
• ★ Molecular Biology
• ★ Protein/Enzyme Engineering
• ☆ Molecular Modeling
• ☆ Liquid Chromatography/HPLC
• ☆ Cell Banking
• ☆ Medicinal Chemistry

サービス

• Writing
• Research
• Consulting
• Product Development

Writing Technical Writing

Research Market Research, User Research, Gap Analysis, Gray Literature Search, Systematic Literature Review

Consulting Scientific and Technical Consulting

Product Development Formulation, Concept Development

職務経験

Postdoctoral Research Fellow

Children's Hospital Boston

6月 2013 - 現在

学歴

Ph.D. (Chemistry)

Tufts University

9月 2004 - 8月 2012

M.Sc. (Chemistry)

Indian Institute of Technology Madras

6月 2001 - 5月 2003

B.Sc. (Chemistry)

Loyola College

7月 1998 - 5月 2001

認定資格

• 認定資格の詳細は未入力です。

出版物

JOURNAL ARTICLE

(2020). Angiogenic responses in a 3D micro-engineered environment of primary endothelial cells and pericytes . Angiogenesis.

Khajavi, M., Zhou, Y., Birsner, A.E., Bazinet, L., Rosa Di Sant, A., Schiffer, A.J., Rogers, M.S., Krishnaji, S.T., Hu, B., Nguyen, V., et al.(2017). Identification of Padi2 as a novel angiogenesis-regulating gene by genome association studies in mice . PLoS Genetics. 13. (6).

Doyle, J.R., Harwood, B.N., Krishnaji, S.T., Krishnamurthy, V.M., Lin, W.-E., Fortin, J.-P., Kumar, K., Kopin, A.S.(2014). A two-step strategy to enhance activity of low potency peptides . PLoS ONE. 9. (11).

Doyle, J.R., Krishnaji, S.T., Zhu, G., Xu, Z.-Z., Heller, D., Ji, R.-R., Levy, B.D., Kumar, K., Kopin, A.S.(2014). Development of a membrane-Anchored chemerin receptor agonist as a novel modulator of allergic airway inflammation and neuropathic pain . Journal of Biological Chemistry. 289. (19). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 13385-13396.

Vuram, P.K., Subuddhi, U., Krishnaji, S.T., Chadha, A., Mishra, A.K.(2010). Synthesis and aggregation properties of dansylated glycerol-based amphiphilic polyether dendrons . European Journal of Organic Chemistry. (26). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 5030-5040.

Meng, H., Krishnaji, S.T., Beinborn, M., Kumar, K.(2008). Influence of selective fluorination on the biological activity and proteolytic stability of glucagon-like peptide-1 . Journal of Medicinal Chemistry. 51. (22). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 7303-7307.